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Acacia salicina extracts protect against DNA damage and mutagenesis in bacteria and human lymphoblast cell K562 cultures.
Nutr Res. 2008 Mar; 28(3):190-7.NR

Abstract

Three extracts were prepared from the leaves of Acacia salicina: aqueous, methanol, and ethyl acetate extracts. The antigenotoxic properties of these extracts were investigated by assessing the inhibition of mutagenicity of the indirect-acting mutagen benzo[a]pyrene using the Ames assay and the genotoxicity of the direct-acting mutagen, hydrogen peroxide, using the "Comet assay." Aqueous, methanol, and ethyl acetate extracts at doses of 500, 50, and 500 microg per plate reduced benzo[a]pyrene mutagenicity by 95%, 82%, and 40%, respectively, in Salmonella typhimurium TA98 strain and by 91%, 66% and 63%, respectively, at the same doses with a TA97 assay system. Human lymphoblast cells K562 were pretreated with 50% inhibition concentration of each extracts and then treated by H(2)O(2), for the Comet assay. The Comet assay results showed that ethyl acetate and methanol extracts decreased the DNA damage caused by H(2)O(2) by, respectively, 34.8% and 31.3%. We envisaged also the study of the antioxidant effect of these extracts by the enzymatic xanthine/xanthine oxidase assay. Results indicated that methanol and ethyl acetate extracts were potent inhibitors of xanthine oxidase and superoxide anion scavengers. We conclude that these integrated approaches to antigenotoxicity and antioxidant assessment may be useful to help compare the beneficial effects associated with using A salicina as medicinal and dietary plant.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Bouhlel I
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03. Faculté de Pharmacie de Monastir, Rue Avicenne 5000 Monastir, Tunisie.
Kilani S
No affiliation info available
Skandrani I
No affiliation info available
Ben Amar R
No affiliation info available
Nefatti A
No affiliation info available
Laporte F
No affiliation info available
Hininger-Favier I
No affiliation info available
Ghedira K
No affiliation info available
Chekir-Ghedira L
No affiliation info available

MeSH

AcaciaAntimutagenic AgentsAntioxidantsCells, CulturedComet AssayDNA DamageDose-Response Relationship, DrugHumansK562 CellsMutagenesisMutagenicity TestsPlant ExtractsPlant LeavesSalmonella typhimurium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19083407

Citation

Bouhlel, Ines, et al. "Acacia Salicina Extracts Protect Against DNA Damage and Mutagenesis in Bacteria and Human Lymphoblast Cell K562 Cultures." Nutrition Research (New York, N.Y.), vol. 28, no. 3, 2008, pp. 190-7.
Bouhlel I, Kilani S, Skandrani I, et al. Acacia salicina extracts protect against DNA damage and mutagenesis in bacteria and human lymphoblast cell K562 cultures. Nutr Res. 2008;28(3):190-7.
Bouhlel, I., Kilani, S., Skandrani, I., Ben Amar, R., Nefatti, A., Laporte, F., Hininger-Favier, I., Ghedira, K., & Chekir-Ghedira, L. (2008). Acacia salicina extracts protect against DNA damage and mutagenesis in bacteria and human lymphoblast cell K562 cultures. Nutrition Research (New York, N.Y.), 28(3), 190-7. https://doi.org/10.1016/j.nutres.2007.12.011
Bouhlel I, et al. Acacia Salicina Extracts Protect Against DNA Damage and Mutagenesis in Bacteria and Human Lymphoblast Cell K562 Cultures. Nutr Res. 2008;28(3):190-7. PubMed PMID: 19083407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acacia salicina extracts protect against DNA damage and mutagenesis in bacteria and human lymphoblast cell K562 cultures. AU - Bouhlel,Ines, AU - Kilani,Soumaya, AU - Skandrani,Ines, AU - Ben Amar,Rebaii, AU - Nefatti,Aicha, AU - Laporte,François, AU - Hininger-Favier,Isabelle, AU - Ghedira,Kamel, AU - Chekir-Ghedira,Leila, PY - 2007/09/07/received PY - 2007/12/02/revised PY - 2007/12/11/accepted PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/3/7/medline SP - 190 EP - 7 JF - Nutrition research (New York, N.Y.) JO - Nutr Res VL - 28 IS - 3 N2 - Three extracts were prepared from the leaves of Acacia salicina: aqueous, methanol, and ethyl acetate extracts. The antigenotoxic properties of these extracts were investigated by assessing the inhibition of mutagenicity of the indirect-acting mutagen benzo[a]pyrene using the Ames assay and the genotoxicity of the direct-acting mutagen, hydrogen peroxide, using the "Comet assay." Aqueous, methanol, and ethyl acetate extracts at doses of 500, 50, and 500 microg per plate reduced benzo[a]pyrene mutagenicity by 95%, 82%, and 40%, respectively, in Salmonella typhimurium TA98 strain and by 91%, 66% and 63%, respectively, at the same doses with a TA97 assay system. Human lymphoblast cells K562 were pretreated with 50% inhibition concentration of each extracts and then treated by H(2)O(2), for the Comet assay. The Comet assay results showed that ethyl acetate and methanol extracts decreased the DNA damage caused by H(2)O(2) by, respectively, 34.8% and 31.3%. We envisaged also the study of the antioxidant effect of these extracts by the enzymatic xanthine/xanthine oxidase assay. Results indicated that methanol and ethyl acetate extracts were potent inhibitors of xanthine oxidase and superoxide anion scavengers. We conclude that these integrated approaches to antigenotoxicity and antioxidant assessment may be useful to help compare the beneficial effects associated with using A salicina as medicinal and dietary plant. SN - 1879-0739 UR - https://www.unboundmedicine.com/medline/citation/19083407/Acacia_salicina_extracts_protect_against_DNA_damage_and_mutagenesis_in_bacteria_and_human_lymphoblast_cell_K562_cultures_ DB - PRIME DP - Unbound Medicine ER -
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