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Latanoprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo.
Exp Eye Res. 2009 Mar; 88(3):535-41.EE

Abstract

We investigated whether latanoprost has a direct anti-apoptotic effect in retinal ganglion cell (RGC) line and RGCs in the rat. RGC-5 cells were induced to undergo apoptosis by serum deprivation and exogenous glutamate. The level of cell death with or without latanoprost acid was monitored by an XTT assay and by immunocytochemistry with activated caspase-3. Changes in the level of intracellular calcium ([Ca(2+)]i) were measured with fluo-4 fluorescence. The XTT assay revealed that latanoprost acid increased RGC-5 cell viability. Latanoprost acid significantly reduced caspase-3 positive cells and suppressed [Ca(2+)]i evoked by glutamate. U0126, a mitogen-activated protein/extracellular signal-regulated kinase 1 and 2 inhibitor, partially blocked the rescue effect of latnanoprost acid (p=0.013). In vivo, rat RGCs were degenerated by optic nerve crush. After topical instillation of latanoprost for 7days, RGCs labeled with fluorogold were significantly. Retinal flatmounts were subjected to terminal dUTP nick end labeling (TUNEL) staining to detect apoptotic cells. TUNEL-positive cells were significantly decreased in eyes with topically instilled latanoprost (p=0.015). These data suggest that latanoprost has an neuroprotective ability in RGCs.

Authors+Show Affiliations

Department of Surgery, Division of Ophthalmology, Kobe University Graduate School of Medicine, Kobe, Japan. kanaaki@med.kobe-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19084521

Citation

Kanamori, Akiyasu, et al. "Latanoprost Protects Rat Retinal Ganglion Cells From Apoptosis in Vitro and in Vivo." Experimental Eye Research, vol. 88, no. 3, 2009, pp. 535-41.
Kanamori A, Naka M, Fukuda M, et al. Latanoprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo. Exp Eye Res. 2009;88(3):535-41.
Kanamori, A., Naka, M., Fukuda, M., Nakamura, M., & Negi, A. (2009). Latanoprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo. Experimental Eye Research, 88(3), 535-41. https://doi.org/10.1016/j.exer.2008.11.012
Kanamori A, et al. Latanoprost Protects Rat Retinal Ganglion Cells From Apoptosis in Vitro and in Vivo. Exp Eye Res. 2009;88(3):535-41. PubMed PMID: 19084521.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Latanoprost protects rat retinal ganglion cells from apoptosis in vitro and in vivo. AU - Kanamori,Akiyasu, AU - Naka,Maiko, AU - Fukuda,Masahide, AU - Nakamura,Makoto, AU - Negi,Akira, Y1 - 2008/12/03/ PY - 2008/05/07/received PY - 2008/11/11/revised PY - 2008/11/11/accepted PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/5/27/medline SP - 535 EP - 41 JF - Experimental eye research JO - Exp Eye Res VL - 88 IS - 3 N2 - We investigated whether latanoprost has a direct anti-apoptotic effect in retinal ganglion cell (RGC) line and RGCs in the rat. RGC-5 cells were induced to undergo apoptosis by serum deprivation and exogenous glutamate. The level of cell death with or without latanoprost acid was monitored by an XTT assay and by immunocytochemistry with activated caspase-3. Changes in the level of intracellular calcium ([Ca(2+)]i) were measured with fluo-4 fluorescence. The XTT assay revealed that latanoprost acid increased RGC-5 cell viability. Latanoprost acid significantly reduced caspase-3 positive cells and suppressed [Ca(2+)]i evoked by glutamate. U0126, a mitogen-activated protein/extracellular signal-regulated kinase 1 and 2 inhibitor, partially blocked the rescue effect of latnanoprost acid (p=0.013). In vivo, rat RGCs were degenerated by optic nerve crush. After topical instillation of latanoprost for 7days, RGCs labeled with fluorogold were significantly. Retinal flatmounts were subjected to terminal dUTP nick end labeling (TUNEL) staining to detect apoptotic cells. TUNEL-positive cells were significantly decreased in eyes with topically instilled latanoprost (p=0.015). These data suggest that latanoprost has an neuroprotective ability in RGCs. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/19084521/Latanoprost_protects_rat_retinal_ganglion_cells_from_apoptosis_in_vitro_and_in_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(08)00396-5 DB - PRIME DP - Unbound Medicine ER -