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Combining biofunctional magnetic nanoparticles and ATP bioluminescence for rapid detection of Escherichia coli.
Talanta. 2009 Feb 15; 77(4):1332-6.T

Abstract

A rapid, specific and sensitive method for assay of Escherichia coli (E. coli) using biofunctional magnetic nanoparticles (BMNPs) in combination with adenosine triphosphate (ATP) bioluminescence was proposed. The BMNPs were fabricated by immobilizing a specific anti-E. coli antibody on the surface of amine-functionalized magnetic nanoparticles (about 20nm in diameter), and then was applied to capture the target bacteria E. coli from samples. The BMNPs exhibited high capture efficiency to E. coli. Transmission electron microscope (TEM) images showed that the BMNPs were bound to the surface of entire E. coli cells. The target bacteria became magnetic so that could be isolated easily from the sample solution by employing an external magnetic field. The concentration of E. coli captured by the BMNPs was then detected by an ATP bioluminescence method. The optimization of ATP measurement was carried out to improve the detection sensitivity. The proposed method was applied to detect the E. coli inoculated into pasteurized milk with low detection limit (20 cfu/mL) and short detection time (about 1h).

Authors+Show Affiliations

Department of Chemistry, East China Normal University, Shanghai, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19084645

Citation

Cheng, Yuxiao, et al. "Combining Biofunctional Magnetic Nanoparticles and ATP Bioluminescence for Rapid Detection of Escherichia Coli." Talanta, vol. 77, no. 4, 2009, pp. 1332-6.
Cheng Y, Liu Y, Huang J, et al. Combining biofunctional magnetic nanoparticles and ATP bioluminescence for rapid detection of Escherichia coli. Talanta. 2009;77(4):1332-6.
Cheng, Y., Liu, Y., Huang, J., Li, K., Zhang, W., Xian, Y., & Jin, L. (2009). Combining biofunctional magnetic nanoparticles and ATP bioluminescence for rapid detection of Escherichia coli. Talanta, 77(4), 1332-6. https://doi.org/10.1016/j.talanta.2008.09.014
Cheng Y, et al. Combining Biofunctional Magnetic Nanoparticles and ATP Bioluminescence for Rapid Detection of Escherichia Coli. Talanta. 2009 Feb 15;77(4):1332-6. PubMed PMID: 19084645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combining biofunctional magnetic nanoparticles and ATP bioluminescence for rapid detection of Escherichia coli. AU - Cheng,Yuxiao, AU - Liu,Yajun, AU - Huang,Jingjing, AU - Li,Kang, AU - Zhang,Wen, AU - Xian,Yuezhong, AU - Jin,Litong, Y1 - 2008/09/18/ PY - 2008/06/11/received PY - 2008/09/01/revised PY - 2008/09/09/accepted PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/6/12/medline SP - 1332 EP - 6 JF - Talanta JO - Talanta VL - 77 IS - 4 N2 - A rapid, specific and sensitive method for assay of Escherichia coli (E. coli) using biofunctional magnetic nanoparticles (BMNPs) in combination with adenosine triphosphate (ATP) bioluminescence was proposed. The BMNPs were fabricated by immobilizing a specific anti-E. coli antibody on the surface of amine-functionalized magnetic nanoparticles (about 20nm in diameter), and then was applied to capture the target bacteria E. coli from samples. The BMNPs exhibited high capture efficiency to E. coli. Transmission electron microscope (TEM) images showed that the BMNPs were bound to the surface of entire E. coli cells. The target bacteria became magnetic so that could be isolated easily from the sample solution by employing an external magnetic field. The concentration of E. coli captured by the BMNPs was then detected by an ATP bioluminescence method. The optimization of ATP measurement was carried out to improve the detection sensitivity. The proposed method was applied to detect the E. coli inoculated into pasteurized milk with low detection limit (20 cfu/mL) and short detection time (about 1h). SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19084645/Combining_biofunctional_magnetic_nanoparticles_and_ATP_bioluminescence_for_rapid_detection_of_Escherichia_coli_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(08)00671-1 DB - PRIME DP - Unbound Medicine ER -