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Trace determination of short-chain aliphatic amines in biological samples by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection.
Talanta. 2009 Feb 15; 77(4):1337-42.T

Abstract

In this study, a new capillary electrophoresis (CE) method is described originally for the sensitive and selective determination of short-chain aliphatic amines in biological samples. These amines were converted into their N-hydroxysuccinimidyl fluorescein-O-acetate (SIFA) derivatives and measured by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. The derivatization conditions and separation parameters for the aliphatic amines were optimized in detail. The SIFA-labeled amines were fully separated within 8.5 min using 25 mM pH 9.6 boric acid electrolyte containing 60mM sodium dodecyl sulfate (SDS). The parameters of validation such as linearity of response, precision and detection limits were determined. The detection limits were obtained in the range from 0.02 to 0.1 nM, which was the lowest value reported by CE methods. The developed method was successfully employed to monitor aliphatic amines in serum and cells samples. After comparison of other CE methods using different fluorescent probes, the present method represents a powerful tool for the trace determination of aliphatic amines in complex biological samples.

Authors+Show Affiliations

Department of Chemistry, Wuhan University, Wuhan, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19084646

Citation

Deng, Ying-Hua, et al. "Trace Determination of Short-chain Aliphatic Amines in Biological Samples By Micellar Electrokinetic Capillary Chromatography With Laser-induced Fluorescence Detection." Talanta, vol. 77, no. 4, 2009, pp. 1337-42.
Deng YH, Wang H, Zhong L, et al. Trace determination of short-chain aliphatic amines in biological samples by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. Talanta. 2009;77(4):1337-42.
Deng, Y. H., Wang, H., Zhong, L., & Zhang, H. S. (2009). Trace determination of short-chain aliphatic amines in biological samples by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. Talanta, 77(4), 1337-42. https://doi.org/10.1016/j.talanta.2008.09.013
Deng YH, et al. Trace Determination of Short-chain Aliphatic Amines in Biological Samples By Micellar Electrokinetic Capillary Chromatography With Laser-induced Fluorescence Detection. Talanta. 2009 Feb 15;77(4):1337-42. PubMed PMID: 19084646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trace determination of short-chain aliphatic amines in biological samples by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. AU - Deng,Ying-Hua, AU - Wang,Hong, AU - Zhong,Lu, AU - Zhang,Hua-Shan, Y1 - 2008/09/18/ PY - 2008/06/18/received PY - 2008/09/07/revised PY - 2008/09/09/accepted PY - 2008/12/17/entrez PY - 2008/12/17/pubmed PY - 2009/6/12/medline SP - 1337 EP - 42 JF - Talanta JO - Talanta VL - 77 IS - 4 N2 - In this study, a new capillary electrophoresis (CE) method is described originally for the sensitive and selective determination of short-chain aliphatic amines in biological samples. These amines were converted into their N-hydroxysuccinimidyl fluorescein-O-acetate (SIFA) derivatives and measured by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. The derivatization conditions and separation parameters for the aliphatic amines were optimized in detail. The SIFA-labeled amines were fully separated within 8.5 min using 25 mM pH 9.6 boric acid electrolyte containing 60mM sodium dodecyl sulfate (SDS). The parameters of validation such as linearity of response, precision and detection limits were determined. The detection limits were obtained in the range from 0.02 to 0.1 nM, which was the lowest value reported by CE methods. The developed method was successfully employed to monitor aliphatic amines in serum and cells samples. After comparison of other CE methods using different fluorescent probes, the present method represents a powerful tool for the trace determination of aliphatic amines in complex biological samples. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19084646/Trace_determination_of_short_chain_aliphatic_amines_in_biological_samples_by_micellar_electrokinetic_capillary_chromatography_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(08)00672-3 DB - PRIME DP - Unbound Medicine ER -