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[HPLC fingerprint of total flavonoids of Folium Apocyni Veneti].
Zhongguo Zhong Yao Za Zhi. 2008 Aug; 33(16):1968-71.ZZ

Abstract

OBJECTIVE

To establish a sensitive and specific HPLC fingerprint for the quality controlling of total flavonoids of Folium Apocyni Veneti.

METHOD

HPlC analysis was performed on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm) with the mixture of solvent A [acetonitrile-phosphoric acid (95:5)] and solvent B (0.05% phosphoric acid) in gradient mode at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 360 nm. The column temperature was set at 25 degrees C and the injection volume was 20 microL.

RESULT

The chromatographic fingerprint of total flavonoids was established which showed 17 characteristic peaks from 7 patches of total flavonoids products. The similarity from different patches was 0.95-1.00 analyzed by the software of 'Computer-aided Similarity Evaluation' and showed high similitude in peak numbers and the retention time. Moreover, comparison of the HPLC profiles of the total flavonoids with the corresponding Folium Apocyni Veneti leaves indicated that they were closely related to each other.

CONCLUSION

The chromatographic fingerprint of the total flavonoids with high specificity and can be used to control its quality and assure the homogenicity for each patch of the total flavonoids.

Authors+Show Affiliations

Hao XL
School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China.
Zhang SQ
No affiliation info available
Wang XJ
No affiliation info available
Li QS
No affiliation info available

MeSH

ApocynumChromatography, High Pressure LiquidDrugs, Chinese HerbalFlavonoidsReproducibility of Results

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19086630

Citation

Hao, Xu-liang, et al. "[HPLC Fingerprint of Total Flavonoids of Folium Apocyni Veneti]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 33, no. 16, 2008, pp. 1968-71.
Hao XL, Zhang SQ, Wang XJ, et al. [HPLC fingerprint of total flavonoids of Folium Apocyni Veneti]. Zhongguo Zhong Yao Za Zhi. 2008;33(16):1968-71.
Hao, X. L., Zhang, S. Q., Wang, X. J., & Li, Q. S. (2008). [HPLC fingerprint of total flavonoids of Folium Apocyni Veneti]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 33(16), 1968-71.
Hao XL, et al. [HPLC Fingerprint of Total Flavonoids of Folium Apocyni Veneti]. Zhongguo Zhong Yao Za Zhi. 2008;33(16):1968-71. PubMed PMID: 19086630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [HPLC fingerprint of total flavonoids of Folium Apocyni Veneti]. AU - Hao,Xu-liang, AU - Zhang,Su-qiong, AU - Wang,Xiao-jian, AU - Li,Qing-shan, PY - 2008/12/18/entrez PY - 2008/12/18/pubmed PY - 2009/5/12/medline SP - 1968 EP - 71 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 33 IS - 16 N2 - OBJECTIVE: To establish a sensitive and specific HPLC fingerprint for the quality controlling of total flavonoids of Folium Apocyni Veneti. METHOD: HPlC analysis was performed on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm) with the mixture of solvent A [acetonitrile-phosphoric acid (95:5)] and solvent B (0.05% phosphoric acid) in gradient mode at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 360 nm. The column temperature was set at 25 degrees C and the injection volume was 20 microL. RESULT: The chromatographic fingerprint of total flavonoids was established which showed 17 characteristic peaks from 7 patches of total flavonoids products. The similarity from different patches was 0.95-1.00 analyzed by the software of 'Computer-aided Similarity Evaluation' and showed high similitude in peak numbers and the retention time. Moreover, comparison of the HPLC profiles of the total flavonoids with the corresponding Folium Apocyni Veneti leaves indicated that they were closely related to each other. CONCLUSION: The chromatographic fingerprint of the total flavonoids with high specificity and can be used to control its quality and assure the homogenicity for each patch of the total flavonoids. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/19086630/[HPLC_fingerprint_of_total_flavonoids_of_Folium_Apocyni_Veneti]_ DB - PRIME DP - Unbound Medicine ER -