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Vitex negundo attenuates calpain activation and cataractogenesis in selenite models.
Exp Eye Res. 2009 Mar; 88(3):575-82.EE

Abstract

Recent investigations have shown that phytochemical antioxidants can scavenge free radicals and prevent various diseases. Cataract is the leading cause of blindness and is associated with oxidative damage of the lens. Selenite-induced cataract in rat pups is an excellent mimic of oxidative stress-induced cataract. Selenite cataract is associated with oxidative stress, loss of calcium homeostasis, calpain activation and protein insolubilization in the lens. Our present study focuses on the isolation of flavonoids from Vitex negundo and to assess its efficacy in preventing these changes in the lens of selenite-induced cataract models. Eight-day-old Sprague-Dawley rat pups were used for the study and divided into four groups: Control (G I), Sodium selenite-induced (G II), Sodium selenite+quercetin treated (G III), Sodium selenite+flavonoids from Vitex negundo (FVN) (G IV). Cataract was induced by a single subcutaneous injection of Sodium selenite (4 mg/Kg body weight) on the 10th day. Treatment groups received quercetin (1.0mg/Kg body weight) and FVN (1.0mg/Kg body weight) intraperitoneally from 8th to 15th day. Cataract was visualized from the 16th day. Morphological examination of the rat lenses revealed no opacification in G I and mild opacification in G III and G IV (stage 1) whereas dense opacification in G II (stage 4-6). The activities of superoxide dismutase (SOD), catalase, Ca(2+)ATPase, concentration of reduced glutathione (GSH) and protein sulfhydryl content were significantly increased in G III and G IV compared to G II, while decreased activities of calpains, lower concentration of calcium and thiobarbituric acid reactive substances (TBARS) were observed in G III and IV as compared to G II. Lens protein profile of water soluble proteins showed normal levels of expression in treated groups compared to that of selenite-induced rats. These results indicate good antioxidant and therapeutic potential of FVN in modulating biochemical parameters against selenite-induced cataract, which have been reported in this paper for the first time.

Authors+Show Affiliations

Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19094987

Citation

Rooban, B N., et al. "Vitex Negundo Attenuates Calpain Activation and Cataractogenesis in Selenite Models." Experimental Eye Research, vol. 88, no. 3, 2009, pp. 575-82.
Rooban BN, Lija Y, Biju PG, et al. Vitex negundo attenuates calpain activation and cataractogenesis in selenite models. Exp Eye Res. 2009;88(3):575-82.
Rooban, B. N., Lija, Y., Biju, P. G., Sasikala, V., Sahasranamam, V., & Abraham, A. (2009). Vitex negundo attenuates calpain activation and cataractogenesis in selenite models. Experimental Eye Research, 88(3), 575-82. https://doi.org/10.1016/j.exer.2008.11.020
Rooban BN, et al. Vitex Negundo Attenuates Calpain Activation and Cataractogenesis in Selenite Models. Exp Eye Res. 2009;88(3):575-82. PubMed PMID: 19094987.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitex negundo attenuates calpain activation and cataractogenesis in selenite models. AU - Rooban,B N, AU - Lija,Y, AU - Biju,P G, AU - Sasikala,V, AU - Sahasranamam,V, AU - Abraham,Annie, Y1 - 2008/12/06/ PY - 2008/01/08/received PY - 2008/11/10/revised PY - 2008/11/13/accepted PY - 2008/12/20/entrez PY - 2008/12/20/pubmed PY - 2009/5/27/medline SP - 575 EP - 82 JF - Experimental eye research JO - Exp Eye Res VL - 88 IS - 3 N2 - Recent investigations have shown that phytochemical antioxidants can scavenge free radicals and prevent various diseases. Cataract is the leading cause of blindness and is associated with oxidative damage of the lens. Selenite-induced cataract in rat pups is an excellent mimic of oxidative stress-induced cataract. Selenite cataract is associated with oxidative stress, loss of calcium homeostasis, calpain activation and protein insolubilization in the lens. Our present study focuses on the isolation of flavonoids from Vitex negundo and to assess its efficacy in preventing these changes in the lens of selenite-induced cataract models. Eight-day-old Sprague-Dawley rat pups were used for the study and divided into four groups: Control (G I), Sodium selenite-induced (G II), Sodium selenite+quercetin treated (G III), Sodium selenite+flavonoids from Vitex negundo (FVN) (G IV). Cataract was induced by a single subcutaneous injection of Sodium selenite (4 mg/Kg body weight) on the 10th day. Treatment groups received quercetin (1.0mg/Kg body weight) and FVN (1.0mg/Kg body weight) intraperitoneally from 8th to 15th day. Cataract was visualized from the 16th day. Morphological examination of the rat lenses revealed no opacification in G I and mild opacification in G III and G IV (stage 1) whereas dense opacification in G II (stage 4-6). The activities of superoxide dismutase (SOD), catalase, Ca(2+)ATPase, concentration of reduced glutathione (GSH) and protein sulfhydryl content were significantly increased in G III and G IV compared to G II, while decreased activities of calpains, lower concentration of calcium and thiobarbituric acid reactive substances (TBARS) were observed in G III and IV as compared to G II. Lens protein profile of water soluble proteins showed normal levels of expression in treated groups compared to that of selenite-induced rats. These results indicate good antioxidant and therapeutic potential of FVN in modulating biochemical parameters against selenite-induced cataract, which have been reported in this paper for the first time. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/19094987/Vitex_negundo_attenuates_calpain_activation_and_cataractogenesis_in_selenite_models_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(08)00403-X DB - PRIME DP - Unbound Medicine ER -