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Comparison of LID versus CID activation modes in tandem mass spectrometry of peptides.
J Mass Spectrom. 2009 May; 44(5):621-32.JM

Abstract

We report our contribution to the systematic investigation of peptide fragmentations performed on high-performance Tof equipment, operating in MS and MS/MS modes, such as ESI-QqTof and MALDI-Tof/Tof instruments that are commonly available today in proteomic laboratories. Whereas the former analyzer's configuration provides low-energy collision-induced dissociations (CID), the latter allows tunable activation methods of the selected parent ion to induce either metastable laser-induced dissociations (LID) or high-energy CID ('gas on spectra LID'). Fragmentation of the monoprotonated ion of 53 peptides (FW 807-2853 g/mol) was undertaken upon low-energy CID on an ESI-QTof mass spectrometer (Waters) as well as high-energy CID and LID conditions on a MALDI Ultraflex mass spectrometer (Bruker). Systematic comparison of MS/MS spectra provided useful information on the performance of each piece of equipment for efficient peptide sequencing and also insights into the observed fragmentation behaviors.

Authors+Show Affiliations

Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-Universités Montpellier 1 et 2, Bâtiment Chimie (17), Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19097045

Citation

Shenar, Nawar, et al. "Comparison of LID Versus CID Activation Modes in Tandem Mass Spectrometry of Peptides." Journal of Mass Spectrometry : JMS, vol. 44, no. 5, 2009, pp. 621-32.
Shenar N, Sommerer N, Martinez J, et al. Comparison of LID versus CID activation modes in tandem mass spectrometry of peptides. J Mass Spectrom. 2009;44(5):621-32.
Shenar, N., Sommerer, N., Martinez, J., & Enjalbal, C. (2009). Comparison of LID versus CID activation modes in tandem mass spectrometry of peptides. Journal of Mass Spectrometry : JMS, 44(5), 621-32. https://doi.org/10.1002/jms.1535
Shenar N, et al. Comparison of LID Versus CID Activation Modes in Tandem Mass Spectrometry of Peptides. J Mass Spectrom. 2009;44(5):621-32. PubMed PMID: 19097045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of LID versus CID activation modes in tandem mass spectrometry of peptides. AU - Shenar,Nawar, AU - Sommerer,Nicolas, AU - Martinez,Jean, AU - Enjalbal,Christine, PY - 2008/12/20/entrez PY - 2008/12/20/pubmed PY - 2009/7/29/medline SP - 621 EP - 32 JF - Journal of mass spectrometry : JMS JO - J Mass Spectrom VL - 44 IS - 5 N2 - We report our contribution to the systematic investigation of peptide fragmentations performed on high-performance Tof equipment, operating in MS and MS/MS modes, such as ESI-QqTof and MALDI-Tof/Tof instruments that are commonly available today in proteomic laboratories. Whereas the former analyzer's configuration provides low-energy collision-induced dissociations (CID), the latter allows tunable activation methods of the selected parent ion to induce either metastable laser-induced dissociations (LID) or high-energy CID ('gas on spectra LID'). Fragmentation of the monoprotonated ion of 53 peptides (FW 807-2853 g/mol) was undertaken upon low-energy CID on an ESI-QTof mass spectrometer (Waters) as well as high-energy CID and LID conditions on a MALDI Ultraflex mass spectrometer (Bruker). Systematic comparison of MS/MS spectra provided useful information on the performance of each piece of equipment for efficient peptide sequencing and also insights into the observed fragmentation behaviors. SN - 1096-9888 UR - https://www.unboundmedicine.com/medline/citation/19097045/Comparison_of_LID_versus_CID_activation_modes_in_tandem_mass_spectrometry_of_peptides_ L2 - https://doi.org/10.1002/jms.1535 DB - PRIME DP - Unbound Medicine ER -