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[Effect of electroacupuncture on NMDAR-2 B mRNA expression in hippocampal tissue in vascular dementia rats].
Zhen Ci Yan Jiu. 2008 Oct; 33(5):301-5.ZC

Abstract

OBJECTIVE

To study the underlying mechanism of electroacupuncture (EA) in improving learning-memory ability in vascular dementia (VD) rats.

METHODS

SD rats were randomized into control, sham operation (sham), VD model, non-acupoint and acupoint groups, with 10 cases in each. VD model was established by occlusion of the bilateral common carotid arteries. Morris water maze test was performed to detect the rats' learning-memory ability. The expression of N-methyl-D-aspartate receptor (NMDAR)-2 B mRNA in hippocampal CA 1 region and dentate gyrus was measured by hybridization in situ. EA (2 Hz, 1-2 mA)was applied to "Baihui" (GV 20), "Dazhui" (GV 14) and bilateral "Shenshu"(BL 23) and non-acupoint (the chest-abdominal juncture between the first and the second lumbar vertebrae) for 20 min, once daily for 30 days.

RESULTS

Self-comparison showed that 6 weeks after modeling, the escape latencies of control and sham groups, and the lingering duration in the 3rd quadrant of water pool of model, non-acupoint and acupoint groups decreased significantly (P < 0.05), while the escape latencies of model, non-acupoint and acupoint groups, and the lingering duration of control and sham groups increased considerably (P < 0.5). No significant differences were found between control and sham groups, and among model, non-acupoint and acupoint groups in the escape latency and the lingering duration in the 3rd quadrant (P > 0.05). On the 11th week after modeling, compared to their individual escape latencies and lingering duration on the 6th week, the escape latencies of controll sham and acupoint groups shortened obviously (P < 0.05), while the lingering duration in the 3"rd quadrant of the same 3 groups postponed significantly (P < 0.05). Compared with model group, the escape latency of acupoint group shortened obviously and the lingering duration increased significantly (P < 0.05). No significant differences were found between control and sham groups, and between model and non-acupoint groups in these two indexes (P > 0.05). Compared to control/sham group, the grey values of NMDAR-2 BmRNA expression in hippocampal CA 1 region and dentate gyrus in model and non-acupoint groups decreased significantly (P < 0.05). Compared to model group, the grey values of NMDAR-2 B mRNA expression in these two brain regions increased markedly (P < 0.05). No significant difference was found between control and sham groups, and between model and non-acupoint groups (P > 0.05).

CONCLUSION

Acupoint-EA is able to improve VD rats' learning-memory ability which may be related to its effect in upregulating the expression of NMDAR-2 B mRNA in hippocampal and dentate gyrus.

Authors+Show Affiliations

Department of Anatomy, Histology and Embryology, Fujian Medical University, Fuzhou 350004, China. linsuijin@163.comNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19097500

Citation

Lin, Sui-Jin, and Wei Wang. "[Effect of Electroacupuncture On NMDAR-2 B mRNA Expression in Hippocampal Tissue in Vascular Dementia Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 33, no. 5, 2008, pp. 301-5.
Lin SJ, Wang W. [Effect of electroacupuncture on NMDAR-2 B mRNA expression in hippocampal tissue in vascular dementia rats]. Zhen Ci Yan Jiu. 2008;33(5):301-5.
Lin, S. J., & Wang, W. (2008). [Effect of electroacupuncture on NMDAR-2 B mRNA expression in hippocampal tissue in vascular dementia rats]. Zhen Ci Yan Jiu = Acupuncture Research, 33(5), 301-5.
Lin SJ, Wang W. [Effect of Electroacupuncture On NMDAR-2 B mRNA Expression in Hippocampal Tissue in Vascular Dementia Rats]. Zhen Ci Yan Jiu. 2008;33(5):301-5. PubMed PMID: 19097500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on NMDAR-2 B mRNA expression in hippocampal tissue in vascular dementia rats]. AU - Lin,Sui-Jin, AU - Wang,Wei, PY - 2008/12/23/entrez PY - 2008/12/23/pubmed PY - 2009/1/14/medline SP - 301 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 33 IS - 5 N2 - OBJECTIVE: To study the underlying mechanism of electroacupuncture (EA) in improving learning-memory ability in vascular dementia (VD) rats. METHODS: SD rats were randomized into control, sham operation (sham), VD model, non-acupoint and acupoint groups, with 10 cases in each. VD model was established by occlusion of the bilateral common carotid arteries. Morris water maze test was performed to detect the rats' learning-memory ability. The expression of N-methyl-D-aspartate receptor (NMDAR)-2 B mRNA in hippocampal CA 1 region and dentate gyrus was measured by hybridization in situ. EA (2 Hz, 1-2 mA)was applied to "Baihui" (GV 20), "Dazhui" (GV 14) and bilateral "Shenshu"(BL 23) and non-acupoint (the chest-abdominal juncture between the first and the second lumbar vertebrae) for 20 min, once daily for 30 days. RESULTS: Self-comparison showed that 6 weeks after modeling, the escape latencies of control and sham groups, and the lingering duration in the 3rd quadrant of water pool of model, non-acupoint and acupoint groups decreased significantly (P < 0.05), while the escape latencies of model, non-acupoint and acupoint groups, and the lingering duration of control and sham groups increased considerably (P < 0.5). No significant differences were found between control and sham groups, and among model, non-acupoint and acupoint groups in the escape latency and the lingering duration in the 3rd quadrant (P > 0.05). On the 11th week after modeling, compared to their individual escape latencies and lingering duration on the 6th week, the escape latencies of controll sham and acupoint groups shortened obviously (P < 0.05), while the lingering duration in the 3"rd quadrant of the same 3 groups postponed significantly (P < 0.05). Compared with model group, the escape latency of acupoint group shortened obviously and the lingering duration increased significantly (P < 0.05). No significant differences were found between control and sham groups, and between model and non-acupoint groups in these two indexes (P > 0.05). Compared to control/sham group, the grey values of NMDAR-2 BmRNA expression in hippocampal CA 1 region and dentate gyrus in model and non-acupoint groups decreased significantly (P < 0.05). Compared to model group, the grey values of NMDAR-2 B mRNA expression in these two brain regions increased markedly (P < 0.05). No significant difference was found between control and sham groups, and between model and non-acupoint groups (P > 0.05). CONCLUSION: Acupoint-EA is able to improve VD rats' learning-memory ability which may be related to its effect in upregulating the expression of NMDAR-2 B mRNA in hippocampal and dentate gyrus. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/19097500/[Effect_of_electroacupuncture_on_NMDAR_2_B_mRNA_expression_in_hippocampal_tissue_in_vascular_dementia_rats]_ DB - PRIME DP - Unbound Medicine ER -