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Tinidazole inhibitory and cidal activity against anaerobic periodontal pathogens.
Int J Antimicrob Agents. 2009 May; 33(5):449-52.IJ

Abstract

The in vitro activity of tinidazole against anaerobic periodontal pathogens (25 Prevotella buccae, 18 Prevotella denticola, 10 Prevotella intermedia, 6 Prevotella melaninogenica, 5 Prevotella oralis, 10 Fusobacterium nucleatum and 8 Veillonella spp.) was determined by agar dilution. MIC(90) values (minimum inhibitory concentration for 90% of the organisms) were 8 microg/mL for Veillonella spp., 4 microg/mL for P. intermedia, 2 microg/mL for P. buccae, 1 microg/mL for Fusobacterium spp. and 0.5 microg/mL for other Prevotella spp. Cidal activity was studied by killing curves with tinidazole and amoxicillin (alone and in combination) at concentrations similar to those achieved in crevicular fluid (41.2 microg/mL tinidazole and 14.05 microg/mL amoxicillin) against an inoculum of ca. 10(7)colony-forming units/mL of four bacterial groups, each one composed of four different strains of the following periodontal isolates: Prevotella spp., Fusobacterium spp. and Veillonella spp. (anaerobes) and one amoxicillin-susceptible Streptococcus spp. (facultative) in a proportion of 1:1:1:1. When only beta-lactamase-negative Prevotella or Fusobacterium strains were tested, significantly higher reductions were found with amoxicillin (>4 log reduction at 48 h) versus controls. The presence of beta-lactamase-positive Prevotella spp. or F. nucleatum strains rendered amoxicillin inactive (no reductions at 48 h), with no differences from controls. Amoxicillin+tinidazole produced >3 log reduction at 24h and >4 log reduction at 48 h regardless of the presence or not of beta-lactamase-positive strains. The presence in crevicular fluid of beta-lactamases produced by beta-lactamase-positive periodontal pathogens may have ecological and therapeutic consequences since it may protect beta-lactamase-negative periodontal pathogens from amoxicillin treatment. In vitro, tinidazole offered high antianaerobic activity against beta-lactamase-positive and -negative periodontal pathogens, avoiding amoxicillin inactivation.

Authors+Show Affiliations

Microbiology Department, School of Medicine, Universidad Complutense, Avenida Complutense s/n, 28040 Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19097755

Citation

Alou, L, et al. "Tinidazole Inhibitory and Cidal Activity Against Anaerobic Periodontal Pathogens." International Journal of Antimicrobial Agents, vol. 33, no. 5, 2009, pp. 449-52.
Alou L, Giménez MJ, Manso F, et al. Tinidazole inhibitory and cidal activity against anaerobic periodontal pathogens. Int J Antimicrob Agents. 2009;33(5):449-52.
Alou, L., Giménez, M. J., Manso, F., Sevillano, D., Torrico, M., González, N., Granizo, J. J., Bascones, A., Prieto, J., Maestre, J. R., & Aguilar, L. (2009). Tinidazole inhibitory and cidal activity against anaerobic periodontal pathogens. International Journal of Antimicrobial Agents, 33(5), 449-52. https://doi.org/10.1016/j.ijantimicag.2008.10.012
Alou L, et al. Tinidazole Inhibitory and Cidal Activity Against Anaerobic Periodontal Pathogens. Int J Antimicrob Agents. 2009;33(5):449-52. PubMed PMID: 19097755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tinidazole inhibitory and cidal activity against anaerobic periodontal pathogens. AU - Alou,L, AU - Giménez,M J, AU - Manso,F, AU - Sevillano,D, AU - Torrico,M, AU - González,N, AU - Granizo,J J, AU - Bascones,A, AU - Prieto,J, AU - Maestre,J R, AU - Aguilar,L, Y1 - 2008/12/20/ PY - 2008/08/02/received PY - 2008/10/01/revised PY - 2008/10/06/accepted PY - 2008/12/23/entrez PY - 2008/12/23/pubmed PY - 2009/4/14/medline SP - 449 EP - 52 JF - International journal of antimicrobial agents JO - Int. J. Antimicrob. Agents VL - 33 IS - 5 N2 - The in vitro activity of tinidazole against anaerobic periodontal pathogens (25 Prevotella buccae, 18 Prevotella denticola, 10 Prevotella intermedia, 6 Prevotella melaninogenica, 5 Prevotella oralis, 10 Fusobacterium nucleatum and 8 Veillonella spp.) was determined by agar dilution. MIC(90) values (minimum inhibitory concentration for 90% of the organisms) were 8 microg/mL for Veillonella spp., 4 microg/mL for P. intermedia, 2 microg/mL for P. buccae, 1 microg/mL for Fusobacterium spp. and 0.5 microg/mL for other Prevotella spp. Cidal activity was studied by killing curves with tinidazole and amoxicillin (alone and in combination) at concentrations similar to those achieved in crevicular fluid (41.2 microg/mL tinidazole and 14.05 microg/mL amoxicillin) against an inoculum of ca. 10(7)colony-forming units/mL of four bacterial groups, each one composed of four different strains of the following periodontal isolates: Prevotella spp., Fusobacterium spp. and Veillonella spp. (anaerobes) and one amoxicillin-susceptible Streptococcus spp. (facultative) in a proportion of 1:1:1:1. When only beta-lactamase-negative Prevotella or Fusobacterium strains were tested, significantly higher reductions were found with amoxicillin (>4 log reduction at 48 h) versus controls. The presence of beta-lactamase-positive Prevotella spp. or F. nucleatum strains rendered amoxicillin inactive (no reductions at 48 h), with no differences from controls. Amoxicillin+tinidazole produced >3 log reduction at 24h and >4 log reduction at 48 h regardless of the presence or not of beta-lactamase-positive strains. The presence in crevicular fluid of beta-lactamases produced by beta-lactamase-positive periodontal pathogens may have ecological and therapeutic consequences since it may protect beta-lactamase-negative periodontal pathogens from amoxicillin treatment. In vitro, tinidazole offered high antianaerobic activity against beta-lactamase-positive and -negative periodontal pathogens, avoiding amoxicillin inactivation. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/19097755/Tinidazole_inhibitory_and_cidal_activity_against_anaerobic_periodontal_pathogens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(08)00521-9 DB - PRIME DP - Unbound Medicine ER -