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Stratum corneum lipid organization as observed by atomic force, confocal and two-photon excitation fluorescence microscopy.
Int J Cosmet Sci. 2008 Dec; 30(6):391-411.IJ

Abstract

Skin moisturization is largely a function of stratum corneum barrier capacity, which in turn is a function of the physical state and structural organization of the stratum corneum extracellular lipid matrix [J. Invest. Dermatol.18, 433 (1952); AIChE J. 21, 985 (1975); Acta Derm. Venereol.74, 1 (1994); J. Invest. Dermatol.117, 830 (2001)]. Three unsolved key questions with respect to this lipid matrix' structural organization [Acta Derm. Venereol.74, 1 (1994); J. Invest. Dermatol.117, 830 (2001); J. Invest. Dermatol.118, 897 (2002); J. Invest. Dermatol.118, 899 (2002)] are: i) whether the lipid matrix is constituted by a single-gel phase or by co-existing solid (crystalline or gel) domains, ii) whether a separate fluid (liquid crystalline) phase is present and iii) whether the local pH has a direct effect on the lipid matrix' phase behaviour. Using an array of complementary visual-related biophysical techniques (e.g. atomic force microscopy and confocal/two-photon excitation fluorescence microscopy), it was recently shown that reconstituted membranes composed of extracted decontaminated human stratum corneum lipids do not form a fluid phase, but exclusively a single-gel phase that segregates into co-existing microscopic domains below pH 6 [Biophys. J.93, 3142 (2007)]. It was further shown that the role of cholesterol is related to dispersion of ceramide-enriched domains. This effect is counteracted by the presence of free fatty acids, which mix with skin ceramides but not with cholesterol.

Authors+Show Affiliations

Department of Cell and Molecular Biology (CMB), Medical Nobel Institute, Karolinska Institute, Stockholm, Sweden. lars.norlen@ki.seNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

19099542

Citation

Norlén, L, et al. "Stratum Corneum Lipid Organization as Observed By Atomic Force, Confocal and Two-photon Excitation Fluorescence Microscopy." International Journal of Cosmetic Science, vol. 30, no. 6, 2008, pp. 391-411.
Norlén L, Plasencia I, Bagatolli L. Stratum corneum lipid organization as observed by atomic force, confocal and two-photon excitation fluorescence microscopy. Int J Cosmet Sci. 2008;30(6):391-411.
Norlén, L., Plasencia, I., & Bagatolli, L. (2008). Stratum corneum lipid organization as observed by atomic force, confocal and two-photon excitation fluorescence microscopy. International Journal of Cosmetic Science, 30(6), 391-411. https://doi.org/10.1111/j.1468-2494.2008.00458.x
Norlén L, Plasencia I, Bagatolli L. Stratum Corneum Lipid Organization as Observed By Atomic Force, Confocal and Two-photon Excitation Fluorescence Microscopy. Int J Cosmet Sci. 2008;30(6):391-411. PubMed PMID: 19099542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stratum corneum lipid organization as observed by atomic force, confocal and two-photon excitation fluorescence microscopy. AU - Norlén,L, AU - Plasencia,I, AU - Bagatolli,L, PY - 2008/12/23/entrez PY - 2008/12/23/pubmed PY - 2009/2/13/medline SP - 391 EP - 411 JF - International journal of cosmetic science JO - Int J Cosmet Sci VL - 30 IS - 6 N2 - Skin moisturization is largely a function of stratum corneum barrier capacity, which in turn is a function of the physical state and structural organization of the stratum corneum extracellular lipid matrix [J. Invest. Dermatol.18, 433 (1952); AIChE J. 21, 985 (1975); Acta Derm. Venereol.74, 1 (1994); J. Invest. Dermatol.117, 830 (2001)]. Three unsolved key questions with respect to this lipid matrix' structural organization [Acta Derm. Venereol.74, 1 (1994); J. Invest. Dermatol.117, 830 (2001); J. Invest. Dermatol.118, 897 (2002); J. Invest. Dermatol.118, 899 (2002)] are: i) whether the lipid matrix is constituted by a single-gel phase or by co-existing solid (crystalline or gel) domains, ii) whether a separate fluid (liquid crystalline) phase is present and iii) whether the local pH has a direct effect on the lipid matrix' phase behaviour. Using an array of complementary visual-related biophysical techniques (e.g. atomic force microscopy and confocal/two-photon excitation fluorescence microscopy), it was recently shown that reconstituted membranes composed of extracted decontaminated human stratum corneum lipids do not form a fluid phase, but exclusively a single-gel phase that segregates into co-existing microscopic domains below pH 6 [Biophys. J.93, 3142 (2007)]. It was further shown that the role of cholesterol is related to dispersion of ceramide-enriched domains. This effect is counteracted by the presence of free fatty acids, which mix with skin ceramides but not with cholesterol. SN - 1468-2494 UR - https://www.unboundmedicine.com/medline/citation/19099542/Stratum_corneum_lipid_organization_as_observed_by_atomic_force_confocal_and_two_photon_excitation_fluorescence_microscopy_ L2 - https://doi.org/10.1111/j.1468-2494.2008.00458.x DB - PRIME DP - Unbound Medicine ER -