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Increased basal transepidermal water loss leads to elevation of some but not all stratum corneum serine proteases.
Int J Cosmet Sci. 2008 Dec; 30(6):435-42.IJ

Abstract

There are indications of elevation of some inflammatory serine proteases in barrier damaged skin (e.g. plasmin and urokinase). Moreover, many other serine protease activities are present such as desquamatory enzymes as well as a newly detected tryptase-like serine protease. However, the activities of these proteases have never been correlated with stratum corneum (SC) barrier function. The activity of extractable key serine proteases (SC trypsin-like kallikreins, SC chymotrypsin-like kallikreins, SC tryptase-like serine protease, urokinase and plasmin) was measured from the outermost layers of SC obtained from facial tape strippings in clinically normal subjects. The protein content of the tape strippings was quantified by absorption measurements with the novel infrared densitometer SquameScan 850A and the protease activities by the use of fluorogenic peptide substrates. SC barrier function, SC hydration and skin surface pH were measured using AquaFlux, NOVA dermal phase meter and Skin-pH-Meter, respectively. As expected, SC hydration was reduced with increased transepidermal water loss (TEWL) values indicative of barrier impairment. Surprisingly, SC chymotrypsin-like activity showed no correlation with hydration or TEWL, whereas all other enzymes positively correlated with impaired barrier function and some were statistically significant: SC trypsin-like kallikreins (R(2)=0.66, P < 0.01), SC tryptase-like enzyme (R(2)=0.95, P < 0.001), plasmin (R(2)=0.86, P < 0.001) and urokinase (R(2)=0.50, P < 0.05). All enzymes except urokinase also negatively correlated with SC hydration. Elevated levels of SC serine proteases have been associated with some dermatological disorders, such as atopic dermatitis, psoriasis and rosacea but these results indicate that these enzymes are also elevated with milder forms of barrier disruption, which is not clinically evident as irritated skin. As these proteases are elevated in the SC, they will also be elevated in the epidermis where they can be involved in neurogenic inflammation and epidermal barrier impairment via activation of the protease-activated receptors. These results highlight the need for using serine protease inhibitors especially for urokinase and plasmin, SC tryptase-like serine protease and possibly SC trypsin-like kallikreins even in milder forms of barrier damage.

Authors+Show Affiliations

DSM Nutritional Products Ltd, Branch Pentapharm, Engelgasse 109, 4002 Basel, Switzerland. rainer.voegeli@pentapharm.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19099544

Citation

Voegeli, R, et al. "Increased Basal Transepidermal Water Loss Leads to Elevation of some but Not All Stratum Corneum Serine Proteases." International Journal of Cosmetic Science, vol. 30, no. 6, 2008, pp. 435-42.
Voegeli R, Rawlings AV, Doppler S, et al. Increased basal transepidermal water loss leads to elevation of some but not all stratum corneum serine proteases. Int J Cosmet Sci. 2008;30(6):435-42.
Voegeli, R., Rawlings, A. V., Doppler, S., & Schreier, T. (2008). Increased basal transepidermal water loss leads to elevation of some but not all stratum corneum serine proteases. International Journal of Cosmetic Science, 30(6), 435-42. https://doi.org/10.1111/j.1468-2494.2008.00472.x
Voegeli R, et al. Increased Basal Transepidermal Water Loss Leads to Elevation of some but Not All Stratum Corneum Serine Proteases. Int J Cosmet Sci. 2008;30(6):435-42. PubMed PMID: 19099544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased basal transepidermal water loss leads to elevation of some but not all stratum corneum serine proteases. AU - Voegeli,R, AU - Rawlings,A V, AU - Doppler,S, AU - Schreier,T, PY - 2008/12/23/entrez PY - 2008/12/23/pubmed PY - 2009/2/13/medline SP - 435 EP - 42 JF - International journal of cosmetic science JO - Int J Cosmet Sci VL - 30 IS - 6 N2 - There are indications of elevation of some inflammatory serine proteases in barrier damaged skin (e.g. plasmin and urokinase). Moreover, many other serine protease activities are present such as desquamatory enzymes as well as a newly detected tryptase-like serine protease. However, the activities of these proteases have never been correlated with stratum corneum (SC) barrier function. The activity of extractable key serine proteases (SC trypsin-like kallikreins, SC chymotrypsin-like kallikreins, SC tryptase-like serine protease, urokinase and plasmin) was measured from the outermost layers of SC obtained from facial tape strippings in clinically normal subjects. The protein content of the tape strippings was quantified by absorption measurements with the novel infrared densitometer SquameScan 850A and the protease activities by the use of fluorogenic peptide substrates. SC barrier function, SC hydration and skin surface pH were measured using AquaFlux, NOVA dermal phase meter and Skin-pH-Meter, respectively. As expected, SC hydration was reduced with increased transepidermal water loss (TEWL) values indicative of barrier impairment. Surprisingly, SC chymotrypsin-like activity showed no correlation with hydration or TEWL, whereas all other enzymes positively correlated with impaired barrier function and some were statistically significant: SC trypsin-like kallikreins (R(2)=0.66, P < 0.01), SC tryptase-like enzyme (R(2)=0.95, P < 0.001), plasmin (R(2)=0.86, P < 0.001) and urokinase (R(2)=0.50, P < 0.05). All enzymes except urokinase also negatively correlated with SC hydration. Elevated levels of SC serine proteases have been associated with some dermatological disorders, such as atopic dermatitis, psoriasis and rosacea but these results indicate that these enzymes are also elevated with milder forms of barrier disruption, which is not clinically evident as irritated skin. As these proteases are elevated in the SC, they will also be elevated in the epidermis where they can be involved in neurogenic inflammation and epidermal barrier impairment via activation of the protease-activated receptors. These results highlight the need for using serine protease inhibitors especially for urokinase and plasmin, SC tryptase-like serine protease and possibly SC trypsin-like kallikreins even in milder forms of barrier damage. SN - 1468-2494 UR - https://www.unboundmedicine.com/medline/citation/19099544/Increased_basal_transepidermal_water_loss_leads_to_elevation_of_some_but_not_all_stratum_corneum_serine_proteases_ L2 - https://doi.org/10.1111/j.1468-2494.2008.00472.x DB - PRIME DP - Unbound Medicine ER -