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Basic fibroblast growth factor-enhanced neurogenesis contributes to cognitive recovery in rats following traumatic brain injury.
Exp Neurol. 2009 Mar; 216(1):56-65.EN

Abstract

Stem/progenitor cells reside throughout the adult CNS and are actively dividing in the subventricular zone (SVZ) and the dentate gyrus (DG) of the hippocampus. This neurogenic capacity of the SVZ and DG is enhanced following traumatic brain injury (TBI) suggesting that the adult brain has the inherent potential to restore populations lost to injury. This raises the possibility of developing strategies aimed at harnessing the neurogenic capacity of these regions to repair the damaged brain. One strategy is to enhance neurogenesis with mitogenic factors. As basic fibroblast growth factor (bFGF) is a potent stem cell mitogen, we set out to determine if an intraventricular administration of bFGF following TBI could affect the levels of injury-induced neurogenesis in the SVZ and DG, and the degree to which this is associated with cognitive recovery. Specifically, adult rats received a bFGF intraventricular infusion for 7 days immediately following TBI. BrdU was administered to animals daily at 2-7 days post-injury to label cell proliferation. At 1 or 4 weeks post-injury, brain sections were immunostained for BrdU and neuronal or astrocytic markers. We found that injured animals infused with bFGF exhibited significantly enhanced cell proliferation in the SVZ and the DG at 1 week post-TBI as compared to vehicle-infused animals. Moreover, following bFGF infusion, a greater number of the newly generated cells survived to 4 weeks post-injury, with the majority being neurons. Additionally, animals infused with bFGF showed significant cognitive improvement. Collectively, the current findings suggest that bFGF-enhanced neurogenesis contributes to cognitive recovery following TBI.

Authors+Show Affiliations

Department of Neurosurgery, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298, USA. dsun@vcu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19100261

Citation

Sun, Dong, et al. "Basic Fibroblast Growth Factor-enhanced Neurogenesis Contributes to Cognitive Recovery in Rats Following Traumatic Brain Injury." Experimental Neurology, vol. 216, no. 1, 2009, pp. 56-65.
Sun D, Bullock MR, McGinn MJ, et al. Basic fibroblast growth factor-enhanced neurogenesis contributes to cognitive recovery in rats following traumatic brain injury. Exp Neurol. 2009;216(1):56-65.
Sun, D., Bullock, M. R., McGinn, M. J., Zhou, Z., Altememi, N., Hagood, S., Hamm, R., & Colello, R. J. (2009). Basic fibroblast growth factor-enhanced neurogenesis contributes to cognitive recovery in rats following traumatic brain injury. Experimental Neurology, 216(1), 56-65. https://doi.org/10.1016/j.expneurol.2008.11.011
Sun D, et al. Basic Fibroblast Growth Factor-enhanced Neurogenesis Contributes to Cognitive Recovery in Rats Following Traumatic Brain Injury. Exp Neurol. 2009;216(1):56-65. PubMed PMID: 19100261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Basic fibroblast growth factor-enhanced neurogenesis contributes to cognitive recovery in rats following traumatic brain injury. AU - Sun,Dong, AU - Bullock,M Ross, AU - McGinn,Melissa J, AU - Zhou,Zhengwen, AU - Altememi,Nabil, AU - Hagood,Sarah, AU - Hamm,Robert, AU - Colello,Raymond J, Y1 - 2008/11/27/ PY - 2008/06/30/received PY - 2008/10/27/revised PY - 2008/11/11/accepted PY - 2008/12/23/entrez PY - 2008/12/23/pubmed PY - 2009/3/21/medline SP - 56 EP - 65 JF - Experimental neurology JO - Exp. Neurol. VL - 216 IS - 1 N2 - Stem/progenitor cells reside throughout the adult CNS and are actively dividing in the subventricular zone (SVZ) and the dentate gyrus (DG) of the hippocampus. This neurogenic capacity of the SVZ and DG is enhanced following traumatic brain injury (TBI) suggesting that the adult brain has the inherent potential to restore populations lost to injury. This raises the possibility of developing strategies aimed at harnessing the neurogenic capacity of these regions to repair the damaged brain. One strategy is to enhance neurogenesis with mitogenic factors. As basic fibroblast growth factor (bFGF) is a potent stem cell mitogen, we set out to determine if an intraventricular administration of bFGF following TBI could affect the levels of injury-induced neurogenesis in the SVZ and DG, and the degree to which this is associated with cognitive recovery. Specifically, adult rats received a bFGF intraventricular infusion for 7 days immediately following TBI. BrdU was administered to animals daily at 2-7 days post-injury to label cell proliferation. At 1 or 4 weeks post-injury, brain sections were immunostained for BrdU and neuronal or astrocytic markers. We found that injured animals infused with bFGF exhibited significantly enhanced cell proliferation in the SVZ and the DG at 1 week post-TBI as compared to vehicle-infused animals. Moreover, following bFGF infusion, a greater number of the newly generated cells survived to 4 weeks post-injury, with the majority being neurons. Additionally, animals infused with bFGF showed significant cognitive improvement. Collectively, the current findings suggest that bFGF-enhanced neurogenesis contributes to cognitive recovery following TBI. SN - 1090-2430 UR - https://www.unboundmedicine.com/medline/citation/19100261/Basic_fibroblast_growth_factor_enhanced_neurogenesis_contributes_to_cognitive_recovery_in_rats_following_traumatic_brain_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4886(08)00435-4 DB - PRIME DP - Unbound Medicine ER -