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Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii.
Food Chem Toxicol. 2009 Feb; 47(2):361-7.FC

Abstract

Roses are one of the most important groups of ornamental plants and their fruits and flowers are used in a wide variety of food, nutritional products and different traditional medicines. The antioxidant activity of methanolic extracts from fresh flowers of three rose species (Rosa damascena, Rosa bourboniana and Rosa brunonii) was evaluated by 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical method. The ability to scavenge DPPH radical was measured by the discoloration of the solution. The methanolic extract from R. brunonii exhibited maximum free-radical-scavenging activity (64.5+/-0.38%) followed by R. bourboniana (51.8+/-0.46%) and R. damascena (43.6+/-0.25%) at 100 microg/ml. Simultaneously, ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was used to study phenolic composition in the methanolic extracts from the fresh flowers of rose species. The phenolic constituents were further investigated by direct infusion-ESI-QTOF-MS/MS in negative ion mode. Characteristic Electrospray ionization tandem mass spectrometry (ESI-MS/MS) spectra with other diagnostic fragment ions generated by retro Diels-Alder (RDA) fragmentation pathways were recorded for the flavonoids. Distinct similarities were observed in the relative distribution of polyphenolic compounds among the three species. The dominance of quercetin, kaempferol and their glycosides was observed in all the three species.

Authors+Show Affiliations

Natural Plant Products Division, Institute of Himalayan Bioresource Technology (CSIR), Palampur, HP 176 061, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19100811

Citation

Kumar, Neeraj, et al. "Antioxidant Activity and Ultra-performance LC-electrospray Ionization-quadrupole Time-of-flight Mass Spectrometry for Phenolics-based Fingerprinting of Rose Species: Rosa Damascena, Rosa Bourboniana and Rosa Brunonii." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 47, no. 2, 2009, pp. 361-7.
Kumar N, Bhandari P, Singh B, et al. Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii. Food Chem Toxicol. 2009;47(2):361-7.
Kumar, N., Bhandari, P., Singh, B., & Bari, S. S. (2009). Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 47(2), 361-7. https://doi.org/10.1016/j.fct.2008.11.036
Kumar N, et al. Antioxidant Activity and Ultra-performance LC-electrospray Ionization-quadrupole Time-of-flight Mass Spectrometry for Phenolics-based Fingerprinting of Rose Species: Rosa Damascena, Rosa Bourboniana and Rosa Brunonii. Food Chem Toxicol. 2009;47(2):361-7. PubMed PMID: 19100811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii. AU - Kumar,Neeraj, AU - Bhandari,Pamita, AU - Singh,Bikram, AU - Bari,Shamsher S, Y1 - 2008/12/07/ PY - 2007/07/19/received PY - 2008/10/10/revised PY - 2008/11/17/accepted PY - 2008/12/23/entrez PY - 2008/12/23/pubmed PY - 2009/2/20/medline SP - 361 EP - 7 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 47 IS - 2 N2 - Roses are one of the most important groups of ornamental plants and their fruits and flowers are used in a wide variety of food, nutritional products and different traditional medicines. The antioxidant activity of methanolic extracts from fresh flowers of three rose species (Rosa damascena, Rosa bourboniana and Rosa brunonii) was evaluated by 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical method. The ability to scavenge DPPH radical was measured by the discoloration of the solution. The methanolic extract from R. brunonii exhibited maximum free-radical-scavenging activity (64.5+/-0.38%) followed by R. bourboniana (51.8+/-0.46%) and R. damascena (43.6+/-0.25%) at 100 microg/ml. Simultaneously, ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was used to study phenolic composition in the methanolic extracts from the fresh flowers of rose species. The phenolic constituents were further investigated by direct infusion-ESI-QTOF-MS/MS in negative ion mode. Characteristic Electrospray ionization tandem mass spectrometry (ESI-MS/MS) spectra with other diagnostic fragment ions generated by retro Diels-Alder (RDA) fragmentation pathways were recorded for the flavonoids. Distinct similarities were observed in the relative distribution of polyphenolic compounds among the three species. The dominance of quercetin, kaempferol and their glycosides was observed in all the three species. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/19100811/Antioxidant_activity_and_ultra_performance_LC_electrospray_ionization_quadrupole_time_of_flight_mass_spectrometry_for_phenolics_based_fingerprinting_of_Rose_species:_Rosa_damascena_Rosa_bourboniana_and_Rosa_brunonii_ DB - PRIME DP - Unbound Medicine ER -