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Determination of urinary androgen glucuronides by capillary electrophoresis with electrospray tandem mass spectrometry.
Biomed Chromatogr. 2009 Apr; 23(4):426-33.BC

Abstract

Capillary electrophoresis-electrospray tandem mass spectrometry (CE-ESI/MS/MS) is a simple and highly sensitive method for quantifying seven urinary androgen glucuronides. The urine samples were diluted and filtered through a membrane filter, and the filtrate was injected into a CE-MS/MS system without further sample preparation steps such as extraction and derivatization. The calibration ranges were 0.01-5 microg/mL for glucuronides of androsterone and 11beta-OHAn-3G, and 5-500 ng/mL for glucuronides of 11-ketoAn, DHEA, testosterone, epitestosterone and DHT. The linearity of the method was 0.992-0.998, and the limits-of-detection at a signal-to-noise ratio of 3 were 5-10 ng/mL. The coefficients of variation were in the range of 4.0-9.0% for intra-day assay and 4.1-9.8% for inter-day assay. The proposed method may be applicable to metabolic profiling in both quantitative and qualitative analysis.

Authors+Show Affiliations

Bioanalysis and Biotransformation Research Center, KIST, Seoul 136-791, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

19101915

Citation

Cho, Sung-Hee, et al. "Determination of Urinary Androgen Glucuronides By Capillary Electrophoresis With Electrospray Tandem Mass Spectrometry." Biomedical Chromatography : BMC, vol. 23, no. 4, 2009, pp. 426-33.
Cho SH, Lee J, Choi MH, et al. Determination of urinary androgen glucuronides by capillary electrophoresis with electrospray tandem mass spectrometry. Biomed Chromatogr. 2009;23(4):426-33.
Cho, S. H., Lee, J., Choi, M. H., Lee, W. Y., & Chung, B. C. (2009). Determination of urinary androgen glucuronides by capillary electrophoresis with electrospray tandem mass spectrometry. Biomedical Chromatography : BMC, 23(4), 426-33. https://doi.org/10.1002/bmc.1132
Cho SH, et al. Determination of Urinary Androgen Glucuronides By Capillary Electrophoresis With Electrospray Tandem Mass Spectrometry. Biomed Chromatogr. 2009;23(4):426-33. PubMed PMID: 19101915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of urinary androgen glucuronides by capillary electrophoresis with electrospray tandem mass spectrometry. AU - Cho,Sung-Hee, AU - Lee,Jeongae, AU - Choi,Man Ho, AU - Lee,Won-Yong, AU - Chung,Bong Chul, PY - 2008/12/23/entrez PY - 2008/12/23/pubmed PY - 2009/6/10/medline SP - 426 EP - 33 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 23 IS - 4 N2 - Capillary electrophoresis-electrospray tandem mass spectrometry (CE-ESI/MS/MS) is a simple and highly sensitive method for quantifying seven urinary androgen glucuronides. The urine samples were diluted and filtered through a membrane filter, and the filtrate was injected into a CE-MS/MS system without further sample preparation steps such as extraction and derivatization. The calibration ranges were 0.01-5 microg/mL for glucuronides of androsterone and 11beta-OHAn-3G, and 5-500 ng/mL for glucuronides of 11-ketoAn, DHEA, testosterone, epitestosterone and DHT. The linearity of the method was 0.992-0.998, and the limits-of-detection at a signal-to-noise ratio of 3 were 5-10 ng/mL. The coefficients of variation were in the range of 4.0-9.0% for intra-day assay and 4.1-9.8% for inter-day assay. The proposed method may be applicable to metabolic profiling in both quantitative and qualitative analysis. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/19101915/Determination_of_urinary_androgen_glucuronides_by_capillary_electrophoresis_with_electrospray_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/bmc.1132 DB - PRIME DP - Unbound Medicine ER -