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CE-MS in metabolomics.
Electrophoresis. 2009 Jan; 30(1):276-91.E

Abstract

An overview of the use of CE-MS in the field of metabolomics is provided. Metabolomics is concerned with the comprehensive analysis of endogenous low-molecular-weight compounds in biological samples. CE-MS has demonstrated to be a powerful technique for the profiling of polar metabolites in biological samples. This review covers the use of various CE separation modes, capillary coatings, MS analyzers, sample preparation techniques, and data analysis methods used in CE-MS for metabolomics. The applicability of CE-MS in metabolomics research is illustrated by giving examples of the analysis of bacterial extracts, plant extracts, urine, plasma, and cerebrospinal fluid samples. The relevant CE-MS metabolomics studies published between 2000 and 2008 are presented in tabular form, including information on sample type and pretreatment and MS detection mode. Future developments with regard to the use of alternative ionization techniques, the use of coupled separation systems and the potential of microchip CE systems for metabolomics are discussed.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ramautar R
Department of Biomedical Analysis, Utrecht University, Sorbonnelaan, Utrecht, The Netherlands.
Somsen GW
No affiliation info available
de Jong GJ
No affiliation info available

MeSH

BacteriaBody FluidsElectrophoresis, CapillaryHumansMass SpectrometryMetabolomeMetabolomicsPlants

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

19107702

Citation

Ramautar, Rawi, et al. "CE-MS in Metabolomics." Electrophoresis, vol. 30, no. 1, 2009, pp. 276-91.
Ramautar R, Somsen GW, de Jong GJ. CE-MS in metabolomics. Electrophoresis. 2009;30(1):276-91.
Ramautar, R., Somsen, G. W., & de Jong, G. J. (2009). CE-MS in metabolomics. Electrophoresis, 30(1), 276-91. https://doi.org/10.1002/elps.200800512
Ramautar R, Somsen GW, de Jong GJ. CE-MS in Metabolomics. Electrophoresis. 2009;30(1):276-91. PubMed PMID: 19107702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CE-MS in metabolomics. AU - Ramautar,Rawi, AU - Somsen,Govert W, AU - de Jong,Gerhardus J, PY - 2008/12/25/entrez PY - 2008/12/25/pubmed PY - 2009/8/8/medline SP - 276 EP - 91 JF - Electrophoresis JO - Electrophoresis VL - 30 IS - 1 N2 - An overview of the use of CE-MS in the field of metabolomics is provided. Metabolomics is concerned with the comprehensive analysis of endogenous low-molecular-weight compounds in biological samples. CE-MS has demonstrated to be a powerful technique for the profiling of polar metabolites in biological samples. This review covers the use of various CE separation modes, capillary coatings, MS analyzers, sample preparation techniques, and data analysis methods used in CE-MS for metabolomics. The applicability of CE-MS in metabolomics research is illustrated by giving examples of the analysis of bacterial extracts, plant extracts, urine, plasma, and cerebrospinal fluid samples. The relevant CE-MS metabolomics studies published between 2000 and 2008 are presented in tabular form, including information on sample type and pretreatment and MS detection mode. Future developments with regard to the use of alternative ionization techniques, the use of coupled separation systems and the potential of microchip CE systems for metabolomics are discussed. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/19107702/CE_MS_in_metabolomics_ L2 - https://doi.org/10.1002/elps.200800512 DB - PRIME DP - Unbound Medicine ER -