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Protective effect of daidzin against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice.

Abstract

This study examined the effects of daidzin, a major isoflavone from Puerariae Radix, on D-galactosamine (D-GalN) and lipopolysaccharide (LPS)-induced liver failure. Mice were given an intraperitoneal injection of daidzin (25, 50, 100 and 200 mg/kg) 1 h before receiving an injection of D-GalN (700 mg/kg)/LPS (10 microg/kg). Daidzin markedly reduced the elevated serum aminotransferase activity and the levels of lipid peroxidation and tumor necrosis factor-alpha. The glutathione content was lower in the D-GalN/LPS group, which was attenuated by daidzin. The daidzin pretreatment attenuated the swollen mitochondria observed in the d-GalN/LPS group. Daidzin attenuated the apoptosis of hepatocytes, which was confirmed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling method and a caspase-3 assay. Overall, these results suggest that the liver protection of daidzin is due to reduced oxidative stress and its antiapoptotic activity.

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  • Authors+Show Affiliations

    ,

    College of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, Korea.

    , , , ,

    Source

    Phytotherapy research : PTR 23:5 2009 May pg 701-6

    MeSH

    Animals
    Apoptosis
    Caspase 3
    Drugs, Chinese Herbal
    Galactosamine
    Glutathione
    Hepatocytes
    Isoflavones
    Lipid Peroxidation
    Lipopolysaccharides
    Liver
    Liver Failure
    Male
    Mice
    Mice, Inbred ICR
    Mitochondrial Swelling
    Oxidative Stress
    Pueraria
    Transaminases
    Tumor Necrosis Factor-alpha

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19107740

    Citation

    Kim, Sung-Hwa, et al. "Protective Effect of Daidzin Against D-galactosamine and Lipopolysaccharide-induced Hepatic Failure in Mice." Phytotherapy Research : PTR, vol. 23, no. 5, 2009, pp. 701-6.
    Kim SH, Heo JH, Kim YS, et al. Protective effect of daidzin against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice. Phytother Res. 2009;23(5):701-6.
    Kim, S. H., Heo, J. H., Kim, Y. S., Kang, S. S., Choi, J. S., & Lee, S. M. (2009). Protective effect of daidzin against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice. Phytotherapy Research : PTR, 23(5), pp. 701-6. doi:10.1002/ptr.2710.
    Kim SH, et al. Protective Effect of Daidzin Against D-galactosamine and Lipopolysaccharide-induced Hepatic Failure in Mice. Phytother Res. 2009;23(5):701-6. PubMed PMID: 19107740.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Protective effect of daidzin against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice. AU - Kim,Sung-Hwa, AU - Heo,Jeong-Haing, AU - Kim,Yeong Shik, AU - Kang,Sam Sik, AU - Choi,Jae Sue, AU - Lee,Sun-Mee, PY - 2008/12/25/entrez PY - 2008/12/25/pubmed PY - 2009/5/23/medline SP - 701 EP - 6 JF - Phytotherapy research : PTR JO - Phytother Res VL - 23 IS - 5 N2 - This study examined the effects of daidzin, a major isoflavone from Puerariae Radix, on D-galactosamine (D-GalN) and lipopolysaccharide (LPS)-induced liver failure. Mice were given an intraperitoneal injection of daidzin (25, 50, 100 and 200 mg/kg) 1 h before receiving an injection of D-GalN (700 mg/kg)/LPS (10 microg/kg). Daidzin markedly reduced the elevated serum aminotransferase activity and the levels of lipid peroxidation and tumor necrosis factor-alpha. The glutathione content was lower in the D-GalN/LPS group, which was attenuated by daidzin. The daidzin pretreatment attenuated the swollen mitochondria observed in the d-GalN/LPS group. Daidzin attenuated the apoptosis of hepatocytes, which was confirmed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling method and a caspase-3 assay. Overall, these results suggest that the liver protection of daidzin is due to reduced oxidative stress and its antiapoptotic activity. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/19107740/Protective_effect_of_daidzin_against_D_galactosamine_and_lipopolysaccharide_induced_hepatic_failure_in_mice_ L2 - https://doi.org/10.1002/ptr.2710 DB - PRIME DP - Unbound Medicine ER -