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Determination of main taxoids in Taxus species by microwave-assisted extraction combined with LC-MS/MS analysis.
J Sep Sci. 2009 Jan; 32(2):192-201.JS

Abstract

A method based on microwave-assisted extraction (MAE) has been developed for the determination of paclitaxel and five related taxoids, namely 10-deacetylbaccatin III (10-DAB III), cephalomannine, 10-deacetylpaclitaxel (10-DAT), 7-xyl-10- deacetylpaclitaxel (7-xyl-10-DAT), and 7-epi-10-deacetylpaclitaxel (7-epi-10-DAT) in Taxus species in this study. The influential parameters of the MAE procedure were optimized, and the optimal conditions were as follows: extraction solvent 80% ethanol solution, solid/liquid ratio 1:10 (g/mL), temperature 50 degrees C, and three extraction cycles, each cycle 10 min. The method validation for LC-MS/MS analysis was performed. The LOD and LOQ were 3.16-9.20 and 12.20-30.45 ng/mL, respectively. Repeatability and reproducibility for the six taxiods with RSD ranged from 2.78 to 3.85% and from 5.26 to 6.60%. The recoveries of the method for the six taxoids were 92.6-105.6%. The developed MAE-LC-MS/MS method was also successfully applied to determine the contents of six taxoids in different Taxus species.

Authors+Show Affiliations

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19107767

Citation

Luo, Hao, et al. "Determination of Main Taxoids in Taxus Species By Microwave-assisted Extraction Combined With LC-MS/MS Analysis." Journal of Separation Science, vol. 32, no. 2, 2009, pp. 192-201.
Luo H, Nie YK, Fu YJ, et al. Determination of main taxoids in Taxus species by microwave-assisted extraction combined with LC-MS/MS analysis. J Sep Sci. 2009;32(2):192-201.
Luo, H., Nie, Y. K., Fu, Y. J., Zu, Y. G., Li, S. M., Liu, W., Zhang, L., Luo, M., Kong, Y., & Li, Z. N. (2009). Determination of main taxoids in Taxus species by microwave-assisted extraction combined with LC-MS/MS analysis. Journal of Separation Science, 32(2), 192-201. https://doi.org/10.1002/jssc.200800527
Luo H, et al. Determination of Main Taxoids in Taxus Species By Microwave-assisted Extraction Combined With LC-MS/MS Analysis. J Sep Sci. 2009;32(2):192-201. PubMed PMID: 19107767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of main taxoids in Taxus species by microwave-assisted extraction combined with LC-MS/MS analysis. AU - Luo,Hao, AU - Nie,Ying-Kun, AU - Fu,Yu-Jie, AU - Zu,Yuan-Gang, AU - Li,Shuang-Ming, AU - Liu,Wei, AU - Zhang,Lin, AU - Luo,Meng, AU - Kong,Yu, AU - Li,Zheng-Nan, PY - 2008/12/25/entrez PY - 2008/12/25/pubmed PY - 2009/4/21/medline SP - 192 EP - 201 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 2 N2 - A method based on microwave-assisted extraction (MAE) has been developed for the determination of paclitaxel and five related taxoids, namely 10-deacetylbaccatin III (10-DAB III), cephalomannine, 10-deacetylpaclitaxel (10-DAT), 7-xyl-10- deacetylpaclitaxel (7-xyl-10-DAT), and 7-epi-10-deacetylpaclitaxel (7-epi-10-DAT) in Taxus species in this study. The influential parameters of the MAE procedure were optimized, and the optimal conditions were as follows: extraction solvent 80% ethanol solution, solid/liquid ratio 1:10 (g/mL), temperature 50 degrees C, and three extraction cycles, each cycle 10 min. The method validation for LC-MS/MS analysis was performed. The LOD and LOQ were 3.16-9.20 and 12.20-30.45 ng/mL, respectively. Repeatability and reproducibility for the six taxiods with RSD ranged from 2.78 to 3.85% and from 5.26 to 6.60%. The recoveries of the method for the six taxoids were 92.6-105.6%. The developed MAE-LC-MS/MS method was also successfully applied to determine the contents of six taxoids in different Taxus species. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19107767/Determination_of_main_taxoids_in_Taxus_species_by_microwave_assisted_extraction_combined_with_LC_MS/MS_analysis_ L2 - https://doi.org/10.1002/jssc.200800527 DB - PRIME DP - Unbound Medicine ER -