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Recombinant fowlpox virus vector-based vaccine completely protects chickens from H5N1 avian influenza virus.
Antiviral Res. 2009 Mar; 81(3):234-8.AR

Abstract

With the widespread presence of influenza virus H5N1 in poultry and wildlife species, particularly migrating birds, vaccination has become an important control strategy for avian influenza (AI). In this study, the immune efficacy and hemagglutination inhibition (HI) antibody responses induced by a recombinant fowlpox virus (FPV) vector-based rFPV-HA-NA vaccine was evaluated in SPF and commercial chickens. Four-week old SPF chickens vaccinated with one dose of vaccine containing 2 x 10(3) plaque forming units (PFU) of virus were completely protected from H5N1 AI virus 1 week after vaccination, and protective immunity lasted for at least 40 weeks. Two-week old commercial layer chickens were vaccinated with the rFPV-HA-NA vaccine and boosted with the same dose of vaccine following an interval of 18 weeks. The HI antibody titers higher than 4log2 lasted for 52 weeks after the booster immunization. We also examined the efficacy of the rFPV-HA-NA vaccine in SPF chickens administrated by different routes. The results showed that effective application of rFPV-HA-NA vaccine in poultry may be restricted to wing-web puncture, intramuscular or subcutaneous injection. These results demonstrate that the rFPV-HA-NA vaccine is effective in the prevention of infection of H5N1 AI virus.

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Authors+Show Affiliations

Qiao C
Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, PR China.
Jiang Y
No affiliation info available
Tian G
No affiliation info available
Wang X
No affiliation info available
Li C
No affiliation info available
Xin X
No affiliation info available
Chen H
No affiliation info available
Yu K
No affiliation info available

MeSH

AnimalsAntibodies, ViralChickensFowlpox virusHemagglutination Inhibition TestsImmunization, SecondaryInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesInfluenza in BirdsSurvival AnalysisVirus Shedding

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19110002

Citation

Qiao, Chuanling, et al. "Recombinant Fowlpox Virus Vector-based Vaccine Completely Protects Chickens From H5N1 Avian Influenza Virus." Antiviral Research, vol. 81, no. 3, 2009, pp. 234-8.
Qiao C, Jiang Y, Tian G, et al. Recombinant fowlpox virus vector-based vaccine completely protects chickens from H5N1 avian influenza virus. Antiviral Res. 2009;81(3):234-8.
Qiao, C., Jiang, Y., Tian, G., Wang, X., Li, C., Xin, X., Chen, H., & Yu, K. (2009). Recombinant fowlpox virus vector-based vaccine completely protects chickens from H5N1 avian influenza virus. Antiviral Research, 81(3), 234-8. https://doi.org/10.1016/j.antiviral.2008.12.002
Qiao C, et al. Recombinant Fowlpox Virus Vector-based Vaccine Completely Protects Chickens From H5N1 Avian Influenza Virus. Antiviral Res. 2009;81(3):234-8. PubMed PMID: 19110002.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant fowlpox virus vector-based vaccine completely protects chickens from H5N1 avian influenza virus. AU - Qiao,Chuanling, AU - Jiang,Yongping, AU - Tian,Guobin, AU - Wang,Xiurong, AU - Li,Chengjun, AU - Xin,Xiaoguang, AU - Chen,Hualan, AU - Yu,Kangzhen, Y1 - 2008/12/25/ PY - 2008/05/29/received PY - 2008/11/27/revised PY - 2008/12/01/accepted PY - 2008/12/27/entrez PY - 2008/12/27/pubmed PY - 2009/3/13/medline SP - 234 EP - 8 JF - Antiviral research JO - Antiviral Res VL - 81 IS - 3 N2 - With the widespread presence of influenza virus H5N1 in poultry and wildlife species, particularly migrating birds, vaccination has become an important control strategy for avian influenza (AI). In this study, the immune efficacy and hemagglutination inhibition (HI) antibody responses induced by a recombinant fowlpox virus (FPV) vector-based rFPV-HA-NA vaccine was evaluated in SPF and commercial chickens. Four-week old SPF chickens vaccinated with one dose of vaccine containing 2 x 10(3) plaque forming units (PFU) of virus were completely protected from H5N1 AI virus 1 week after vaccination, and protective immunity lasted for at least 40 weeks. Two-week old commercial layer chickens were vaccinated with the rFPV-HA-NA vaccine and boosted with the same dose of vaccine following an interval of 18 weeks. The HI antibody titers higher than 4log2 lasted for 52 weeks after the booster immunization. We also examined the efficacy of the rFPV-HA-NA vaccine in SPF chickens administrated by different routes. The results showed that effective application of rFPV-HA-NA vaccine in poultry may be restricted to wing-web puncture, intramuscular or subcutaneous injection. These results demonstrate that the rFPV-HA-NA vaccine is effective in the prevention of infection of H5N1 AI virus. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/19110002/Recombinant_fowlpox_virus_vector_based_vaccine_completely_protects_chickens_from_H5N1_avian_influenza_virus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-3542(08)00461-0 DB - PRIME DP - Unbound Medicine ER -