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Exogenous galanin regulates capsaicin-evoked substance P release from primary cultured dorsal root ganglion neurons.
Neuro Endocrinol Lett. 2008 Dec; 29(6):911-6.NE

Abstract

OBJECTIVES

To determine the effect of exogenous galanin on capsaicin-evoked substance P (SP) release from primary cultured embryonic rat dorsal root ganglion (DRG) neurons.

METHODS

DRG was dissected out from embryonic 15-day-old Wistar rat and cultured as dissociated cells for 2 days then exposed to galanin (1 nmol/L, 10 nmol/L, 100 nmol/L). After 4 days incubation with exogenous galanin, the levels of mRNAs for SP and vanilloid receptor 1 (VR1) and protein for VR1 were estimated by RT-PCR and Western blot, respectively. Basal SP release and capsaicin-evoked SP release levels were measured by radioimmunoassay (RIA).

RESULTS

The amount of VR1 mRNA and VR1 protein expression and capsaicin-evoked SP release in cultured DRG neurons increased significantly after incubation with exogenous galanin compared with control DRG neurons at the same time point, whereas the amount of SP mRNA and basal SP release were not affected after incubation with exogenous galanin.

DISCUSSION

Exogenous galanin may promote capsaicin-evoked SP release from primary cultured DRG neurons. The elevation of the levels of VR1 mRNA and VR1 protein induced by exogenous galanin implicated that VR1 may be involved in the mechanisms of SP release evoked by capsaicin.

Authors+Show Affiliations

Department of Nephrology, Shandong University Qilu Hospital, Jinan 250012, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19112407

Citation

Yang, Xiangdong, et al. "Exogenous Galanin Regulates Capsaicin-evoked Substance P Release From Primary Cultured Dorsal Root Ganglion Neurons." Neuro Endocrinology Letters, vol. 29, no. 6, 2008, pp. 911-6.
Yang X, Liu Z, Wang L, et al. Exogenous galanin regulates capsaicin-evoked substance P release from primary cultured dorsal root ganglion neurons. Neuro Endocrinol Lett. 2008;29(6):911-6.
Yang, X., Liu, Z., Wang, L., Liu, H., Wang, H., & Li, Z. Z. (2008). Exogenous galanin regulates capsaicin-evoked substance P release from primary cultured dorsal root ganglion neurons. Neuro Endocrinology Letters, 29(6), 911-6.
Yang X, et al. Exogenous Galanin Regulates Capsaicin-evoked Substance P Release From Primary Cultured Dorsal Root Ganglion Neurons. Neuro Endocrinol Lett. 2008;29(6):911-6. PubMed PMID: 19112407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exogenous galanin regulates capsaicin-evoked substance P release from primary cultured dorsal root ganglion neurons. AU - Yang,Xiangdong, AU - Liu,Zhen, AU - Wang,Lihong, AU - Liu,Huaxiang, AU - Wang,Huaijing, AU - Li,Zhen-Zhong, PY - 2007/12/02/received PY - 2008/10/25/accepted PY - 2008/12/30/entrez PY - 2008/12/30/pubmed PY - 2009/4/4/medline SP - 911 EP - 6 JF - Neuro endocrinology letters JO - Neuro Endocrinol Lett VL - 29 IS - 6 N2 - OBJECTIVES: To determine the effect of exogenous galanin on capsaicin-evoked substance P (SP) release from primary cultured embryonic rat dorsal root ganglion (DRG) neurons. METHODS: DRG was dissected out from embryonic 15-day-old Wistar rat and cultured as dissociated cells for 2 days then exposed to galanin (1 nmol/L, 10 nmol/L, 100 nmol/L). After 4 days incubation with exogenous galanin, the levels of mRNAs for SP and vanilloid receptor 1 (VR1) and protein for VR1 were estimated by RT-PCR and Western blot, respectively. Basal SP release and capsaicin-evoked SP release levels were measured by radioimmunoassay (RIA). RESULTS: The amount of VR1 mRNA and VR1 protein expression and capsaicin-evoked SP release in cultured DRG neurons increased significantly after incubation with exogenous galanin compared with control DRG neurons at the same time point, whereas the amount of SP mRNA and basal SP release were not affected after incubation with exogenous galanin. DISCUSSION: Exogenous galanin may promote capsaicin-evoked SP release from primary cultured DRG neurons. The elevation of the levels of VR1 mRNA and VR1 protein induced by exogenous galanin implicated that VR1 may be involved in the mechanisms of SP release evoked by capsaicin. SN - 0172-780X UR - https://www.unboundmedicine.com/medline/citation/19112407/Exogenous_galanin_regulates_capsaicin_evoked_substance_P_release_from_primary_cultured_dorsal_root_ganglion_neurons_ DB - PRIME DP - Unbound Medicine ER -