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Apoptosis patterns in eutopic and ectopic endometrium, adhesions and normal-looking peritoneum from women with or without endometriosis.
Arch Gynecol Obstet. 2009 Aug; 280(2):195-9.AG

Abstract

OBJECTIVE

To assess the apoptosis rate in eutopic and ectopic endometrial stromal and glandular cells, normal peritoneum and adhesions in women with endometriosis.

METHODS

A total number of 97 women with (n:60) and without (n:37) histopathologically confirmed endometriosis who underwent laparoscopy or laparotomy in the early follicular phase of the menstrual cycles for pain and infertility were included in this study. Stage I/II and stage III/IV were categorized as early staged and late-staged endometriosis. The endometrial samples were obtained with a Novack cannula from the corpus of the uterus. Normal-looking peritoneum, peritoneal implants and adhesions were sampled and fixed in formaldehyde for immunohistochemical staining with Bcl-2 and Bax. Tissue samples were fixed in formaldehyde for the assessment of apoptosis via terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) and M30 cytoDEATH antibody.

RESULTS

The intensity of Bax staining of normal-looking peritoneum in early staged endometriosis was higher, compared to women with late-staged and women without endometriosis (P = 0.03). However, degree of Bcl-2 staining did not differ among early and late-staged endometriosis and women without endometriosis (P = 0.1). In terms of Bcl-2 and Bax staining in the stromal and glandular parts of the eutopic endometria, no significant differences were detected among three groups. In cases with early- and late-staged endometriosis the intensity of Bax and Bcl-2 stainings did not differ in both stromal and glandular parts of ectopic endometria. Number of cells with positive apoptotic signals assessed via TUNEL (P = 1.0) and M30 cytoDEATH antibody (P = 0.59) in normal-looking peritoneum did not differ between three groups. In addition, no difference in term of numbers of apoptotic cells obtained from adhesions was observed between three groups (for TUNEL, P = 0.29, for M30, P = 0.19).

CONCLUSIONS

Apoptosis patterns did not differ in the eutopic and ectopic endometria as well as adhesions of women with or without endometriosis.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Eskisehir Osmangazi University School of Medicine, Meselik Kampusu, Eskisehir, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19112572

Citation

Hassa, H, et al. "Apoptosis Patterns in Eutopic and Ectopic Endometrium, Adhesions and Normal-looking Peritoneum From Women With or Without Endometriosis." Archives of Gynecology and Obstetrics, vol. 280, no. 2, 2009, pp. 195-9.
Hassa H, Tanir HM, Tekin B, et al. Apoptosis patterns in eutopic and ectopic endometrium, adhesions and normal-looking peritoneum from women with or without endometriosis. Arch Gynecol Obstet. 2009;280(2):195-9.
Hassa, H., Tanir, H. M., Tekin, B., Artan, S., Dundar, E., Kirilmaz, S. D., & Sahin Mutlu, F. (2009). Apoptosis patterns in eutopic and ectopic endometrium, adhesions and normal-looking peritoneum from women with or without endometriosis. Archives of Gynecology and Obstetrics, 280(2), 195-9. https://doi.org/10.1007/s00404-008-0895-x
Hassa H, et al. Apoptosis Patterns in Eutopic and Ectopic Endometrium, Adhesions and Normal-looking Peritoneum From Women With or Without Endometriosis. Arch Gynecol Obstet. 2009;280(2):195-9. PubMed PMID: 19112572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoptosis patterns in eutopic and ectopic endometrium, adhesions and normal-looking peritoneum from women with or without endometriosis. AU - Hassa,H, AU - Tanir,H Mete, AU - Tekin,B, AU - Artan,S, AU - Dundar,E, AU - Kirilmaz,S D, AU - Sahin Mutlu,F, Y1 - 2008/12/27/ PY - 2008/09/09/received PY - 2008/12/09/accepted PY - 2008/12/30/entrez PY - 2008/12/30/pubmed PY - 2009/9/25/medline SP - 195 EP - 9 JF - Archives of gynecology and obstetrics JO - Arch Gynecol Obstet VL - 280 IS - 2 N2 - OBJECTIVE: To assess the apoptosis rate in eutopic and ectopic endometrial stromal and glandular cells, normal peritoneum and adhesions in women with endometriosis. METHODS: A total number of 97 women with (n:60) and without (n:37) histopathologically confirmed endometriosis who underwent laparoscopy or laparotomy in the early follicular phase of the menstrual cycles for pain and infertility were included in this study. Stage I/II and stage III/IV were categorized as early staged and late-staged endometriosis. The endometrial samples were obtained with a Novack cannula from the corpus of the uterus. Normal-looking peritoneum, peritoneal implants and adhesions were sampled and fixed in formaldehyde for immunohistochemical staining with Bcl-2 and Bax. Tissue samples were fixed in formaldehyde for the assessment of apoptosis via terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) and M30 cytoDEATH antibody. RESULTS: The intensity of Bax staining of normal-looking peritoneum in early staged endometriosis was higher, compared to women with late-staged and women without endometriosis (P = 0.03). However, degree of Bcl-2 staining did not differ among early and late-staged endometriosis and women without endometriosis (P = 0.1). In terms of Bcl-2 and Bax staining in the stromal and glandular parts of the eutopic endometria, no significant differences were detected among three groups. In cases with early- and late-staged endometriosis the intensity of Bax and Bcl-2 stainings did not differ in both stromal and glandular parts of ectopic endometria. Number of cells with positive apoptotic signals assessed via TUNEL (P = 1.0) and M30 cytoDEATH antibody (P = 0.59) in normal-looking peritoneum did not differ between three groups. In addition, no difference in term of numbers of apoptotic cells obtained from adhesions was observed between three groups (for TUNEL, P = 0.29, for M30, P = 0.19). CONCLUSIONS: Apoptosis patterns did not differ in the eutopic and ectopic endometria as well as adhesions of women with or without endometriosis. SN - 1432-0711 UR - https://www.unboundmedicine.com/medline/citation/19112572/Apoptosis_patterns_in_eutopic_and_ectopic_endometrium_adhesions_and_normal_looking_peritoneum_from_women_with_or_without_endometriosis_ L2 - https://dx.doi.org/10.1007/s00404-008-0895-x DB - PRIME DP - Unbound Medicine ER -