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[Study on fingerprint spectrum of Lonicera macranthoides by HPLC].
Zhong Yao Cai. 2008 Aug; 31(8):1138-42.ZY

Abstract

OBJECT

To establish the fingerprint spectrum of Lonicera macranthoides by HPLC.

METHODS

The column of Hypersil gold C18 (4. 6 mm x 250 mm, 5 microm) was used. The mobile phase consisted of Acetonitril-0.05% phosphoric acid with gradient elution. The column temperature was 30 degrees C, the detective wavelength was 240 nm, and the flow rate was 0.5 ml/min.

RESULTS

Fingerprint spectrum of Lonicera macranthoides was established, and 11 samples of different origin Lonicera macranthoides were detected. Ten peaks in the chromatogram were common by Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine (Version 2004 A). There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine.

CONCLUSION

This method is accurate, reliable and provides a scientific basis for controlling the quality of Lonicera macranthoides.

Authors+Show Affiliations

Xin N
Guangxi Traditional Chinese Medical University, Nanning 530001, China. xn@gxtcmu.edu.cn
Feng J
No affiliation info available
Huang LL
No affiliation info available
Yin SG
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDrugs, Chinese HerbalFlowersLoniceraPlants, MedicinalQuality ControlReproducibility of Results

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19112889

Citation

Xin, Ning, et al. "[Study On Fingerprint Spectrum of Lonicera Macranthoides By HPLC]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 31, no. 8, 2008, pp. 1138-42.
Xin N, Feng J, Huang LL, et al. [Study on fingerprint spectrum of Lonicera macranthoides by HPLC]. Zhong Yao Cai. 2008;31(8):1138-42.
Xin, N., Feng, J., Huang, L. L., & Yin, S. G. (2008). [Study on fingerprint spectrum of Lonicera macranthoides by HPLC]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 31(8), 1138-42.
Xin N, et al. [Study On Fingerprint Spectrum of Lonicera Macranthoides By HPLC]. Zhong Yao Cai. 2008;31(8):1138-42. PubMed PMID: 19112889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on fingerprint spectrum of Lonicera macranthoides by HPLC]. AU - Xin,Ning, AU - Feng,Jie, AU - Huang,Lu-liang, AU - Yin,Sheng-gao, PY - 2008/12/31/entrez PY - 2008/12/31/pubmed PY - 2009/12/16/medline SP - 1138 EP - 42 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 31 IS - 8 N2 - OBJECT: To establish the fingerprint spectrum of Lonicera macranthoides by HPLC. METHODS: The column of Hypersil gold C18 (4. 6 mm x 250 mm, 5 microm) was used. The mobile phase consisted of Acetonitril-0.05% phosphoric acid with gradient elution. The column temperature was 30 degrees C, the detective wavelength was 240 nm, and the flow rate was 0.5 ml/min. RESULTS: Fingerprint spectrum of Lonicera macranthoides was established, and 11 samples of different origin Lonicera macranthoides were detected. Ten peaks in the chromatogram were common by Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine (Version 2004 A). There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine. CONCLUSION: This method is accurate, reliable and provides a scientific basis for controlling the quality of Lonicera macranthoides. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/19112889/[Study_on_fingerprint_spectrum_of_Lonicera_macranthoides_by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -