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Transcriptional and functional differences in stem cell populations isolated from extraocular and limb muscles.
Physiol Genomics. 2009 Mar 03; 37(1):35-42.PG

Abstract

The extraocular muscles (EOMs) are a distinct muscle group that displays an array of unique contractile, structural, and regenerative properties. They also have differential sensitivity to certain diseases and are enigmatically spared in Duchenne muscular dystrophy (DMD). The EOMs are so distinct from other skeletal muscles that the term "allotype" has been coined to highlight EOM group-specific properties. We hypothesized that increased and distinct stem cells may underlie the continual myogenesis noted in EOM. The side population (SP) stem cells were isolated and studied. EOMs had 15x higher SP cell content compared with limb muscles. Expression profiling revealed 348 transcripts that define the EOM-SP transcriptome. Over 92% of transcripts were SP specific, because they were absent in previous whole muscle microarray studies. Cultured EOM-SP cells revealed superior in vitro proliferative capacity. Finally, assays of the committed progenitors or satellite cells performed on myofibers isolated from EOM and limb muscles independently validated the increased proliferative capacity of these muscles. We suggest a model in which unique EOM stem cells contribute to the continual myogenesis noted in EOM and consistent with a role for their sparing in DMD. We believe the greater numbers of stem cells, their unique transcriptome, the greater proliferative capacity of EOM stem cells, and the greater number of satellite cells also offer clues for novel cell-based therapeutic strategies.

Authors+Show Affiliations

Department of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6085, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19116248

Citation

Pacheco-Pinedo, Eugenia C., et al. "Transcriptional and Functional Differences in Stem Cell Populations Isolated From Extraocular and Limb Muscles." Physiological Genomics, vol. 37, no. 1, 2009, pp. 35-42.
Pacheco-Pinedo EC, Budak MT, Zeiger U, et al. Transcriptional and functional differences in stem cell populations isolated from extraocular and limb muscles. Physiol Genomics. 2009;37(1):35-42.
Pacheco-Pinedo, E. C., Budak, M. T., Zeiger, U., Jørgensen, L. H., Bogdanovich, S., Schrøder, H. D., Rubinstein, N. A., & Khurana, T. S. (2009). Transcriptional and functional differences in stem cell populations isolated from extraocular and limb muscles. Physiological Genomics, 37(1), 35-42. https://doi.org/10.1152/physiolgenomics.00051.2008
Pacheco-Pinedo EC, et al. Transcriptional and Functional Differences in Stem Cell Populations Isolated From Extraocular and Limb Muscles. Physiol Genomics. 2009 Mar 3;37(1):35-42. PubMed PMID: 19116248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional and functional differences in stem cell populations isolated from extraocular and limb muscles. AU - Pacheco-Pinedo,Eugenia C, AU - Budak,Murat T, AU - Zeiger,Ulrike, AU - Jørgensen,Louise Helskov, AU - Bogdanovich,Sasha, AU - Schrøder,Henrik Daa, AU - Rubinstein,Neal A, AU - Khurana,Tejvir S, Y1 - 2008/12/30/ PY - 2009/1/1/entrez PY - 2009/1/1/pubmed PY - 2009/6/23/medline SP - 35 EP - 42 JF - Physiological genomics JO - Physiol Genomics VL - 37 IS - 1 N2 - The extraocular muscles (EOMs) are a distinct muscle group that displays an array of unique contractile, structural, and regenerative properties. They also have differential sensitivity to certain diseases and are enigmatically spared in Duchenne muscular dystrophy (DMD). The EOMs are so distinct from other skeletal muscles that the term "allotype" has been coined to highlight EOM group-specific properties. We hypothesized that increased and distinct stem cells may underlie the continual myogenesis noted in EOM. The side population (SP) stem cells were isolated and studied. EOMs had 15x higher SP cell content compared with limb muscles. Expression profiling revealed 348 transcripts that define the EOM-SP transcriptome. Over 92% of transcripts were SP specific, because they were absent in previous whole muscle microarray studies. Cultured EOM-SP cells revealed superior in vitro proliferative capacity. Finally, assays of the committed progenitors or satellite cells performed on myofibers isolated from EOM and limb muscles independently validated the increased proliferative capacity of these muscles. We suggest a model in which unique EOM stem cells contribute to the continual myogenesis noted in EOM and consistent with a role for their sparing in DMD. We believe the greater numbers of stem cells, their unique transcriptome, the greater proliferative capacity of EOM stem cells, and the greater number of satellite cells also offer clues for novel cell-based therapeutic strategies. SN - 1531-2267 UR - https://www.unboundmedicine.com/medline/citation/19116248/Transcriptional_and_functional_differences_in_stem_cell_populations_isolated_from_extraocular_and_limb_muscles_ L2 - https://journals.physiology.org/doi/10.1152/physiolgenomics.00051.2008?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -