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Electrostatic interaction based approach to thrombin detection by surface-enhanced Raman spectroscopy.
Anal Chem. 2009 Jan 01; 81(1):87-93.AC

Abstract

We have developed an electrostatic interaction based biosensor for thrombin detection using surface-enhanced Raman spectroscopy (SERS). This method utilized the electrostatic interaction between capture (thrombin aptamer) and probe (crystal violet, CV) molecules. The specific interaction between thrombin and aptamer could weaken the electrostatic barrier effect from the negative charged aptamer SAMs to the diffusion process of the positively charged CV from the bulk solution to the Au nanoparticle surface. Therefore, the more the bound thrombin, the more the CV molecules near the Au nanoparticle surface and the stronger the observed Raman signal of CV, provided the Raman detections were set at the same time point for each case. This procedure presented a highly specific selectivity and a linear detection of thrombin in the range from 0.1 nM to 10 nM with a detection limit of about 20 pM and realized the thrombin detection in human blood serum solution directly. The electrostatic interaction based technique provides an easy and fast-responding optical platform for a "signal-on" detection of proteins, which might be applicable for the real time assay of proteins.

Authors+Show Affiliations

State Key Laboratory of Chem/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University, 410082 Changsha, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19117446

Citation

Hu, Juan, et al. "Electrostatic Interaction Based Approach to Thrombin Detection By Surface-enhanced Raman Spectroscopy." Analytical Chemistry, vol. 81, no. 1, 2009, pp. 87-93.
Hu J, Zheng PC, Jiang JH, et al. Electrostatic interaction based approach to thrombin detection by surface-enhanced Raman spectroscopy. Anal Chem. 2009;81(1):87-93.
Hu, J., Zheng, P. C., Jiang, J. H., Shen, G. L., Yu, R. Q., & Liu, G. K. (2009). Electrostatic interaction based approach to thrombin detection by surface-enhanced Raman spectroscopy. Analytical Chemistry, 81(1), 87-93. https://doi.org/10.1021/ac801431m
Hu J, et al. Electrostatic Interaction Based Approach to Thrombin Detection By Surface-enhanced Raman Spectroscopy. Anal Chem. 2009 Jan 1;81(1):87-93. PubMed PMID: 19117446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrostatic interaction based approach to thrombin detection by surface-enhanced Raman spectroscopy. AU - Hu,Juan, AU - Zheng,Peng-Cheng, AU - Jiang,Jian-Hui, AU - Shen,Guo-Li, AU - Yu,Ru-Qin, AU - Liu,Guo-Kun, PY - 2009/1/2/entrez PY - 2009/1/2/pubmed PY - 2009/2/10/medline SP - 87 EP - 93 JF - Analytical chemistry JO - Anal Chem VL - 81 IS - 1 N2 - We have developed an electrostatic interaction based biosensor for thrombin detection using surface-enhanced Raman spectroscopy (SERS). This method utilized the electrostatic interaction between capture (thrombin aptamer) and probe (crystal violet, CV) molecules. The specific interaction between thrombin and aptamer could weaken the electrostatic barrier effect from the negative charged aptamer SAMs to the diffusion process of the positively charged CV from the bulk solution to the Au nanoparticle surface. Therefore, the more the bound thrombin, the more the CV molecules near the Au nanoparticle surface and the stronger the observed Raman signal of CV, provided the Raman detections were set at the same time point for each case. This procedure presented a highly specific selectivity and a linear detection of thrombin in the range from 0.1 nM to 10 nM with a detection limit of about 20 pM and realized the thrombin detection in human blood serum solution directly. The electrostatic interaction based technique provides an easy and fast-responding optical platform for a "signal-on" detection of proteins, which might be applicable for the real time assay of proteins. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/19117446/Electrostatic_interaction_based_approach_to_thrombin_detection_by_surface_enhanced_Raman_spectroscopy_ L2 - https://doi.org/10.1021/ac801431m DB - PRIME DP - Unbound Medicine ER -