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Multiplex immunoassay using fluorescent-surface enhanced Raman spectroscopic dots for the detection of bronchioalveolar stem cells in murine lung.
Anal Chem. 2009 Feb 01; 81(3):1008-15.AC

Abstract

Immunoassays using nanomaterials have been rapidly developed for the analysis of multiple biomolecules. Highly sensitive and biocompatible surface enhanced Raman spectroscopy-active nanomaterials have been used for biomolecule analysis by many research groups in order to overcome intrinsic problems of conventional immunoassays. We used fluorescent surface-enhanced Raman spectroscopic dots (F-SERS dots) to detect biomolecules in this study. The F-SERS dots are composed of silver nanoparticle-embedded silica nanospheres, organic Raman tagging materials, and fluorescent dyes. The F-SERS dots demonstrated highly sensitive, selective, and multifunctional characteristics for multiplex targeting, tracking, and imaging of cellular and molecular events in the living organism. We successfully applied F-SERS dots for the detection of three cellular proteins, including CD34, Sca-1, and SP-C. These proteins are simultaneously expressed in bronchioalveolar stem cells (BASCs) in the murine lung. We analyzed the relative expression ratios of each protein in BASCs since external standards were used to evaluate SERS intensity in tissue. Quantitative comparisons of multiple protein expression in tissue were first attempted using SERS-encoded nanoprobes. Our results suggested that immunoassays using F-SERS dots offered significant increases in sensitivity and selectivity. Such immunoassays may serve as the primary next-generation labeling technologies for the simultaneous analysis of multiple biomolecules.

Authors+Show Affiliations

College of Veterinary Medicine and Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-742, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19117480

Citation

Woo, Min-Ah, et al. "Multiplex Immunoassay Using Fluorescent-surface Enhanced Raman Spectroscopic Dots for the Detection of Bronchioalveolar Stem Cells in Murine Lung." Analytical Chemistry, vol. 81, no. 3, 2009, pp. 1008-15.
Woo MA, Lee SM, Kim G, et al. Multiplex immunoassay using fluorescent-surface enhanced Raman spectroscopic dots for the detection of bronchioalveolar stem cells in murine lung. Anal Chem. 2009;81(3):1008-15.
Woo, M. A., Lee, S. M., Kim, G., Baek, J., Noh, M. S., Kim, J. E., Park, S. J., Minai-Tehrani, A., Park, S. C., Seo, Y. T., Kim, Y. K., Lee, Y. S., Jeong, D. H., & Cho, M. H. (2009). Multiplex immunoassay using fluorescent-surface enhanced Raman spectroscopic dots for the detection of bronchioalveolar stem cells in murine lung. Analytical Chemistry, 81(3), 1008-15. https://doi.org/10.1021/ac802037x
Woo MA, et al. Multiplex Immunoassay Using Fluorescent-surface Enhanced Raman Spectroscopic Dots for the Detection of Bronchioalveolar Stem Cells in Murine Lung. Anal Chem. 2009 Feb 1;81(3):1008-15. PubMed PMID: 19117480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex immunoassay using fluorescent-surface enhanced Raman spectroscopic dots for the detection of bronchioalveolar stem cells in murine lung. AU - Woo,Min-Ah, AU - Lee,Sang-Myung, AU - Kim,Gunsung, AU - Baek,JongHo, AU - Noh,Mi Suk, AU - Kim,Ji Eun, AU - Park,Sung Jin, AU - Minai-Tehrani,Arash, AU - Park,Se-Chang, AU - Seo,Yeong Tai, AU - Kim,Yong-Kwon, AU - Lee,Yoon-Sik, AU - Jeong,Dae Hong, AU - Cho,Myung-Haing, PY - 2009/1/2/entrez PY - 2009/1/2/pubmed PY - 2009/3/5/medline SP - 1008 EP - 15 JF - Analytical chemistry JO - Anal Chem VL - 81 IS - 3 N2 - Immunoassays using nanomaterials have been rapidly developed for the analysis of multiple biomolecules. Highly sensitive and biocompatible surface enhanced Raman spectroscopy-active nanomaterials have been used for biomolecule analysis by many research groups in order to overcome intrinsic problems of conventional immunoassays. We used fluorescent surface-enhanced Raman spectroscopic dots (F-SERS dots) to detect biomolecules in this study. The F-SERS dots are composed of silver nanoparticle-embedded silica nanospheres, organic Raman tagging materials, and fluorescent dyes. The F-SERS dots demonstrated highly sensitive, selective, and multifunctional characteristics for multiplex targeting, tracking, and imaging of cellular and molecular events in the living organism. We successfully applied F-SERS dots for the detection of three cellular proteins, including CD34, Sca-1, and SP-C. These proteins are simultaneously expressed in bronchioalveolar stem cells (BASCs) in the murine lung. We analyzed the relative expression ratios of each protein in BASCs since external standards were used to evaluate SERS intensity in tissue. Quantitative comparisons of multiple protein expression in tissue were first attempted using SERS-encoded nanoprobes. Our results suggested that immunoassays using F-SERS dots offered significant increases in sensitivity and selectivity. Such immunoassays may serve as the primary next-generation labeling technologies for the simultaneous analysis of multiple biomolecules. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/19117480/Multiplex_immunoassay_using_fluorescent_surface_enhanced_Raman_spectroscopic_dots_for_the_detection_of_bronchioalveolar_stem_cells_in_murine_lung_ L2 - https://doi.org/10.1021/ac802037x DB - PRIME DP - Unbound Medicine ER -