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Cytokine gene polymorphisms and BALF cytokine levels in interstitial lung diseases.
Respir Med. 2009 May; 103(5):773-9.RM

Abstract

BACKGROUND

The aim of our study is to investigate correlations of T(H)1/T(H)2 cytokine gene polymorphisms and bronchoalveolar lavage fluid (BALF) cytokine values in interstitial lung diseases (ILD).

METHODS

In 16 sarcoidosis, 7 idiopathic pulmonary fibrosis (IPF) and 8 hypersensitivity pneumonitis (HP) patients we evaluated IL-1 alpha, -1R, -1RA, -2, -4, -4R alpha, -6, -10, -12, IFN-gamma, TGF-beta1 and TNF-alpha gene polymorphisms in peripheral blood, and MCP-1,MIP-1 alpha, MIP-1 beta, RANTES, ENA-78, FGF, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha, IL-1RA, IL-1 beta, -2, -4, -5, -6, -8, -10, -17, TNF-alpha, Tpo and VEGF values in BALF.

RESULTS

We found higher TNF-alpha values in IL-1R pst 1970 TT homozygotes regardless of diagnosis (p=0.0126). In the sarcoidosis group IL-4R alpha(+1902)AA and IL-10(-1082)G allele correlated with higher BALF ENA-78 levels (p=0.0258, p=0.0230). In the HP group the IL-6(-174)CG and IL-6(nt565)AG correlated with higher ENA-78 BALF levels (p=0.0253). In the IPF group the IL-1 beta +3962 CC homozygotes had lower IL-1RA BALF values (p=0.046). BALF chemokine values did not differ between ILD subgroups, except for IL-8, which was higher in stage III sarcoidosis patients compared to stage I.

CONCLUSION

Our results show a probable influence of gene polymorphisms, namely IL-4R alpha and IL-10 on ENA-78 BALF levels in sarcoidosis, IL-6 on ENA-78 BALF levels in HP and IL-1-beta on IL-1RA BALF values in the IPF group. The TNF-alpha BALF values correlated with IL-1R pst 1970 gene polymorphisms.

Authors+Show Affiliations

Department of Respiratory Diseases, 1st Medical School, Charles University, University Thomayer Hospital, Videnska 800, 140 59 Prague 4, Czech Republic. martina.vasakova@ftn.czNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19117745

Citation

Vasakova, Martina, et al. "Cytokine Gene Polymorphisms and BALF Cytokine Levels in Interstitial Lung Diseases." Respiratory Medicine, vol. 103, no. 5, 2009, pp. 773-9.
Vasakova M, Sterclova M, Kolesar L, et al. Cytokine gene polymorphisms and BALF cytokine levels in interstitial lung diseases. Respir Med. 2009;103(5):773-9.
Vasakova, M., Sterclova, M., Kolesar, L., Slavcev, A., Pohunek, P., Sulc, J., & Striz, I. (2009). Cytokine gene polymorphisms and BALF cytokine levels in interstitial lung diseases. Respiratory Medicine, 103(5), 773-9. https://doi.org/10.1016/j.rmed.2008.11.006
Vasakova M, et al. Cytokine Gene Polymorphisms and BALF Cytokine Levels in Interstitial Lung Diseases. Respir Med. 2009;103(5):773-9. PubMed PMID: 19117745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytokine gene polymorphisms and BALF cytokine levels in interstitial lung diseases. AU - Vasakova,Martina, AU - Sterclova,Martina, AU - Kolesar,Libor, AU - Slavcev,Antonij, AU - Pohunek,Petr, AU - Sulc,Jan, AU - Striz,Ilja, Y1 - 2008/12/30/ PY - 2008/09/16/received PY - 2008/10/30/revised PY - 2008/11/14/accepted PY - 2009/1/2/entrez PY - 2009/1/2/pubmed PY - 2010/3/17/medline SP - 773 EP - 9 JF - Respiratory medicine JO - Respir Med VL - 103 IS - 5 N2 - BACKGROUND: The aim of our study is to investigate correlations of T(H)1/T(H)2 cytokine gene polymorphisms and bronchoalveolar lavage fluid (BALF) cytokine values in interstitial lung diseases (ILD). METHODS: In 16 sarcoidosis, 7 idiopathic pulmonary fibrosis (IPF) and 8 hypersensitivity pneumonitis (HP) patients we evaluated IL-1 alpha, -1R, -1RA, -2, -4, -4R alpha, -6, -10, -12, IFN-gamma, TGF-beta1 and TNF-alpha gene polymorphisms in peripheral blood, and MCP-1,MIP-1 alpha, MIP-1 beta, RANTES, ENA-78, FGF, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha, IL-1RA, IL-1 beta, -2, -4, -5, -6, -8, -10, -17, TNF-alpha, Tpo and VEGF values in BALF. RESULTS: We found higher TNF-alpha values in IL-1R pst 1970 TT homozygotes regardless of diagnosis (p=0.0126). In the sarcoidosis group IL-4R alpha(+1902)AA and IL-10(-1082)G allele correlated with higher BALF ENA-78 levels (p=0.0258, p=0.0230). In the HP group the IL-6(-174)CG and IL-6(nt565)AG correlated with higher ENA-78 BALF levels (p=0.0253). In the IPF group the IL-1 beta +3962 CC homozygotes had lower IL-1RA BALF values (p=0.046). BALF chemokine values did not differ between ILD subgroups, except for IL-8, which was higher in stage III sarcoidosis patients compared to stage I. CONCLUSION: Our results show a probable influence of gene polymorphisms, namely IL-4R alpha and IL-10 on ENA-78 BALF levels in sarcoidosis, IL-6 on ENA-78 BALF levels in HP and IL-1-beta on IL-1RA BALF values in the IPF group. The TNF-alpha BALF values correlated with IL-1R pst 1970 gene polymorphisms. SN - 1532-3064 UR - https://www.unboundmedicine.com/medline/citation/19117745/Cytokine_gene_polymorphisms_and_BALF_cytokine_levels_in_interstitial_lung_diseases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(08)00401-0 DB - PRIME DP - Unbound Medicine ER -