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Determination of kanamycin A, amikacin and tobramycin residues in milk by capillary zone electrophoresis with post-column derivatization and laser-induced fluorescence detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15; 877(3):333-8.JC

Abstract

An analytical method for kanamycin A, amikacin and tobramycin of aminoglycoside (AG) antibiotics in milk samples was proposed using capillary zone electrophoresis (CZE) with post-column derivatization and laser-induced fluorescence detection. A simple and convenient homemade coaxial-gap reactor was adopted in the post-column derivatization of the AGs with 1.0 mmol/L naphthalene-2,3-dicarboxaldehyde and 8.0 mmol/L 2-mercaptoethanol in 35 mmol/L sodium tetraborate buffer (pH 10.0) of 30% (v/v) methanol. 50 mmol/L sodium acetate (pH 5.0) containing 0.5 mmol/L cetyltrimethyl ammonium bromide was used as the separation buffer. The linear calibration concentrations and detection limits were from 2.1 x 10(-5) to 5.0 x 10(-2)g/L and in the range of 7 x 10(-6) to 2 x 10(-5)g/L, respectively. The recoveries of the AGs in milk samples were from 81.6 to 93.1% (n=3).

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Authors+Show Affiliations

Yu CZ
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
He YZ
No affiliation info available
Fu GN
No affiliation info available
Xie HY
No affiliation info available
Gan WE
No affiliation info available

MeSH

AmikacinAnimalsAnti-Bacterial AgentsDrug ResiduesElectrophoresis, CapillaryFluorescenceHydrogen-Ion ConcentrationKanamycinLinear ModelsMilkSensitivity and SpecificityTobramycin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19117808

Citation

Yu, Chang-Zhu, et al. "Determination of Kanamycin A, Amikacin and Tobramycin Residues in Milk By Capillary Zone Electrophoresis With Post-column Derivatization and Laser-induced Fluorescence Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 3, 2009, pp. 333-8.
Yu CZ, He YZ, Fu GN, et al. Determination of kanamycin A, amikacin and tobramycin residues in milk by capillary zone electrophoresis with post-column derivatization and laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(3):333-8.
Yu, C. Z., He, Y. Z., Fu, G. N., Xie, H. Y., & Gan, W. E. (2009). Determination of kanamycin A, amikacin and tobramycin residues in milk by capillary zone electrophoresis with post-column derivatization and laser-induced fluorescence detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877(3), 333-8. https://doi.org/10.1016/j.jchromb.2008.12.011
Yu CZ, et al. Determination of Kanamycin A, Amikacin and Tobramycin Residues in Milk By Capillary Zone Electrophoresis With Post-column Derivatization and Laser-induced Fluorescence Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15;877(3):333-8. PubMed PMID: 19117808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of kanamycin A, amikacin and tobramycin residues in milk by capillary zone electrophoresis with post-column derivatization and laser-induced fluorescence detection. AU - Yu,Chang-Zhu, AU - He,You-Zhao, AU - Fu,Guo-Ni, AU - Xie,Hai-Yang, AU - Gan,Wu-Er, Y1 - 2008/12/11/ PY - 2008/01/26/received PY - 2008/09/22/revised PY - 2008/12/04/accepted PY - 2009/1/2/entrez PY - 2009/1/2/pubmed PY - 2009/4/29/medline SP - 333 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 877 IS - 3 N2 - An analytical method for kanamycin A, amikacin and tobramycin of aminoglycoside (AG) antibiotics in milk samples was proposed using capillary zone electrophoresis (CZE) with post-column derivatization and laser-induced fluorescence detection. A simple and convenient homemade coaxial-gap reactor was adopted in the post-column derivatization of the AGs with 1.0 mmol/L naphthalene-2,3-dicarboxaldehyde and 8.0 mmol/L 2-mercaptoethanol in 35 mmol/L sodium tetraborate buffer (pH 10.0) of 30% (v/v) methanol. 50 mmol/L sodium acetate (pH 5.0) containing 0.5 mmol/L cetyltrimethyl ammonium bromide was used as the separation buffer. The linear calibration concentrations and detection limits were from 2.1 x 10(-5) to 5.0 x 10(-2)g/L and in the range of 7 x 10(-6) to 2 x 10(-5)g/L, respectively. The recoveries of the AGs in milk samples were from 81.6 to 93.1% (n=3). SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/19117808/Determination_of_kanamycin_A_amikacin_and_tobramycin_residues_in_milk_by_capillary_zone_electrophoresis_with_post_column_derivatization_and_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00909-4 DB - PRIME DP - Unbound Medicine ER -