Tags

Type your tag names separated by a space and hit enter

Alteration of testicular steroidogenesis and histopathology of reproductive system in male rats treated with triclosan.
Reprod Toxicol. 2009 Apr; 27(2):177-85.RT

Abstract

Triclosan (TCS), a chlorophenol, is widely used as a preservative in different types of commercial preparations. The reports on TCS-mediated endocrine disruption are controversial and the present study aimed to elucidate the probable mode of action of TCS as an antiandrogenic compound using a robust study design. Male albino rats, Rattus norvegicus, were treated with three doses of triclosan for a period of 60 days followed by the analysis of various biochemical parameters. RT-PCR analysis demonstrated a significant decrease in mRNA levels for testicular steroidogenic acute regulatory (StAR) protein, cytochrome P450(SCC), cytochrome P450(C17), 3beta-hydroxysteroid dehydrogenase (3beta-HSD), 17beta-hydroxysteroid dehydrogenase (17beta-HSD) and androgen receptor (AR) in TCS treated rats (p<0.05). TCS also induced a perturbed translation of testicular StAR, and AR proteins as shown by Western blot analysis in treated groups of rats. A reduced level of StAR was further indicated by immunohistochemistry in testicular Leydig cells. Further, there was a significant decrease (p<0.05) in the level of serum lutenizing hormone (LH), follicle stimulating hormone (FSH), cholesterol, pregnenolone, and testosterone. In vitro assays demonstrated more than 30% decrease in testicular 3beta-HSD and 17beta-HSD enzyme activities in treated group of animals. Extensive histopathological malformations were observed in the testis and sex accessory tissues of the treated rats. Overall this study showed that TCS decreased the synthesis of androgens followed by reduced sperm production in treated male rats which could be mediated by a decreased synthesis of LH and FSH thus involving hypothalamo-pituitary-gonadal axis.

Authors+Show Affiliations

Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19118620

Citation

Kumar, Vikas, et al. "Alteration of Testicular Steroidogenesis and Histopathology of Reproductive System in Male Rats Treated With Triclosan." Reproductive Toxicology (Elmsford, N.Y.), vol. 27, no. 2, 2009, pp. 177-85.
Kumar V, Chakraborty A, Kural MR, et al. Alteration of testicular steroidogenesis and histopathology of reproductive system in male rats treated with triclosan. Reprod Toxicol. 2009;27(2):177-85.
Kumar, V., Chakraborty, A., Kural, M. R., & Roy, P. (2009). Alteration of testicular steroidogenesis and histopathology of reproductive system in male rats treated with triclosan. Reproductive Toxicology (Elmsford, N.Y.), 27(2), 177-85. https://doi.org/10.1016/j.reprotox.2008.12.002
Kumar V, et al. Alteration of Testicular Steroidogenesis and Histopathology of Reproductive System in Male Rats Treated With Triclosan. Reprod Toxicol. 2009;27(2):177-85. PubMed PMID: 19118620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alteration of testicular steroidogenesis and histopathology of reproductive system in male rats treated with triclosan. AU - Kumar,Vikas, AU - Chakraborty,Ajanta, AU - Kural,Mool Raj, AU - Roy,Partha, Y1 - 2008/12/11/ PY - 2008/05/07/received PY - 2008/11/10/revised PY - 2008/12/03/accepted PY - 2009/1/3/entrez PY - 2009/1/3/pubmed PY - 2009/6/3/medline SP - 177 EP - 85 JF - Reproductive toxicology (Elmsford, N.Y.) JO - Reprod Toxicol VL - 27 IS - 2 N2 - Triclosan (TCS), a chlorophenol, is widely used as a preservative in different types of commercial preparations. The reports on TCS-mediated endocrine disruption are controversial and the present study aimed to elucidate the probable mode of action of TCS as an antiandrogenic compound using a robust study design. Male albino rats, Rattus norvegicus, were treated with three doses of triclosan for a period of 60 days followed by the analysis of various biochemical parameters. RT-PCR analysis demonstrated a significant decrease in mRNA levels for testicular steroidogenic acute regulatory (StAR) protein, cytochrome P450(SCC), cytochrome P450(C17), 3beta-hydroxysteroid dehydrogenase (3beta-HSD), 17beta-hydroxysteroid dehydrogenase (17beta-HSD) and androgen receptor (AR) in TCS treated rats (p<0.05). TCS also induced a perturbed translation of testicular StAR, and AR proteins as shown by Western blot analysis in treated groups of rats. A reduced level of StAR was further indicated by immunohistochemistry in testicular Leydig cells. Further, there was a significant decrease (p<0.05) in the level of serum lutenizing hormone (LH), follicle stimulating hormone (FSH), cholesterol, pregnenolone, and testosterone. In vitro assays demonstrated more than 30% decrease in testicular 3beta-HSD and 17beta-HSD enzyme activities in treated group of animals. Extensive histopathological malformations were observed in the testis and sex accessory tissues of the treated rats. Overall this study showed that TCS decreased the synthesis of androgens followed by reduced sperm production in treated male rats which could be mediated by a decreased synthesis of LH and FSH thus involving hypothalamo-pituitary-gonadal axis. SN - 0890-6238 UR - https://www.unboundmedicine.com/medline/citation/19118620/Alteration_of_testicular_steroidogenesis_and_histopathology_of_reproductive_system_in_male_rats_treated_with_triclosan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0890-6238(08)00364-X DB - PRIME DP - Unbound Medicine ER -