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A QCM-D-based biosensor for E. coli O157:H7 highlighting the relevance of the dissipation slope as a transduction signal.
Biosens Bioelectron. 2009 Mar 15; 24(7):2137-42.BB

Abstract

A biosensor for detection of viable Escherichia coli (E. coli) O157:H7 is developed using a quartz crystal microbalance with dissipation monitoring (QCM-D). The detection platform is based on the immobilization of affinity purified polyclonal antibodies onto gold-coated QCM-D quartz crystals via a cysteamine self-assembled monolayer. QCM-D measurements conducted over a broad range of bacterial cell concentrations show that the optimal biosensor response is the initial slope of the dissipation shift as a function of elapsed time (D(slope)). A highly log-log linear response in the initial D(slope) is obtained for detection of E. coli O157:H7 over a wide range of cell concentrations from 3 x 10(5) to 1 x 10(9)cells/mL. The prepared biosensor also exhibits a log-log linear working range from 10(7) to 10(9)cells/mL for E. coli K12 D21, a non-pathogenic model organism and further shows satisfactory selectivity using Bacillus subtilis. To our knowledge, this is the first study demonstrating the use of the initial D(slope) as a sensor response when using QCM-D technology.

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Authors+Show Affiliations

Poitras C
Department of Chemical Engineering, McGill University, Montreal, Quebec H3A 2B2, Canada.
Tufenkji N
No affiliation info available

MeSH

AlgorithmsBiosensing TechniquesColony Count, MicrobialEquipment DesignEquipment Failure AnalysisEscherichia coli O157ImmunoassayMicro-Electrical-Mechanical SystemsReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19118996

Citation

Poitras, Charles, and Nathalie Tufenkji. "A QCM-D-based Biosensor for E. Coli O157:H7 Highlighting the Relevance of the Dissipation Slope as a Transduction Signal." Biosensors & Bioelectronics, vol. 24, no. 7, 2009, pp. 2137-42.
Poitras C, Tufenkji N. A QCM-D-based biosensor for E. coli O157:H7 highlighting the relevance of the dissipation slope as a transduction signal. Biosens Bioelectron. 2009;24(7):2137-42.
Poitras, C., & Tufenkji, N. (2009). A QCM-D-based biosensor for E. coli O157:H7 highlighting the relevance of the dissipation slope as a transduction signal. Biosensors & Bioelectronics, 24(7), 2137-42. https://doi.org/10.1016/j.bios.2008.11.016
Poitras C, Tufenkji N. A QCM-D-based Biosensor for E. Coli O157:H7 Highlighting the Relevance of the Dissipation Slope as a Transduction Signal. Biosens Bioelectron. 2009 Mar 15;24(7):2137-42. PubMed PMID: 19118996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A QCM-D-based biosensor for E. coli O157:H7 highlighting the relevance of the dissipation slope as a transduction signal. AU - Poitras,Charles, AU - Tufenkji,Nathalie, Y1 - 2008/11/27/ PY - 2008/09/18/received PY - 2008/10/27/revised PY - 2008/11/11/accepted PY - 2009/1/3/entrez PY - 2009/1/3/pubmed PY - 2009/5/27/medline SP - 2137 EP - 42 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 7 N2 - A biosensor for detection of viable Escherichia coli (E. coli) O157:H7 is developed using a quartz crystal microbalance with dissipation monitoring (QCM-D). The detection platform is based on the immobilization of affinity purified polyclonal antibodies onto gold-coated QCM-D quartz crystals via a cysteamine self-assembled monolayer. QCM-D measurements conducted over a broad range of bacterial cell concentrations show that the optimal biosensor response is the initial slope of the dissipation shift as a function of elapsed time (D(slope)). A highly log-log linear response in the initial D(slope) is obtained for detection of E. coli O157:H7 over a wide range of cell concentrations from 3 x 10(5) to 1 x 10(9)cells/mL. The prepared biosensor also exhibits a log-log linear working range from 10(7) to 10(9)cells/mL for E. coli K12 D21, a non-pathogenic model organism and further shows satisfactory selectivity using Bacillus subtilis. To our knowledge, this is the first study demonstrating the use of the initial D(slope) as a sensor response when using QCM-D technology. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19118996/A_QCM_D_based_biosensor_for_E__coli_O157:H7_highlighting_the_relevance_of_the_dissipation_slope_as_a_transduction_signal_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00615-5 DB - PRIME DP - Unbound Medicine ER -