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Three distinct condensin complexes control C. elegans chromosome dynamics.
Curr Biol. 2009 Jan 13; 19(1):9-19.CB

Abstract

BACKGROUND

Condensin complexes organize chromosome structure and facilitate chromosome segregation. Higher eukaryotes have two complexes, condensin I and condensin II, each essential for chromosome segregation. The nematode Caenorhabditis elegans was considered an exception, because it has a mitotic condensin II complex but appeared to lack mitotic condensin I. Instead, its condensin I-like complex (here called condensin I(DC)) dampens gene expression along hermaphrodite X chromosomes during dosage compensation.

RESULTS

Here we report the discovery of a third condensin complex, condensin I, in C. elegans. We identify new condensin subunits and show that each complex has a conserved five-subunit composition. Condensin I differs from condensin I(DC) by only a single subunit. Yet condensin I binds to autosomes and X chromosomes in both sexes to promote chromosome segregation, whereas condensin I(DC) binds specifically to X chromosomes in hermaphrodites to regulate transcript levels. Both condensin I and II promote chromosome segregation, but associate with different chromosomal regions during mitosis and meiosis. Unexpectedly, condensin I also localizes to regions of cohesion between meiotic chromosomes before their segregation.

CONCLUSIONS

We demonstrate that condensin subunits in C. elegans form three complexes, one that functions in dosage compensation and two that function in mitosis and meiosis. These results highlight how the duplication and divergence of condensin subunits during evolution may facilitate their adaptation to specialized chromosomal roles and illustrate the versatility of condensins to function in both gene regulation and chromosome segregation.

Authors+Show Affiliations

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. gyorgyi@umich.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19119011

Citation

Csankovszki, Gyorgyi, et al. "Three Distinct Condensin Complexes Control C. Elegans Chromosome Dynamics." Current Biology : CB, vol. 19, no. 1, 2009, pp. 9-19.
Csankovszki G, Collette K, Spahl K, et al. Three distinct condensin complexes control C. elegans chromosome dynamics. Curr Biol. 2009;19(1):9-19.
Csankovszki, G., Collette, K., Spahl, K., Carey, J., Snyder, M., Petty, E., Patel, U., Tabuchi, T., Liu, H., McLeod, I., Thompson, J., Sarkeshik, A., Sarkesik, A., Yates, J., Meyer, B. J., & Hagstrom, K. (2009). Three distinct condensin complexes control C. elegans chromosome dynamics. Current Biology : CB, 19(1), 9-19. https://doi.org/10.1016/j.cub.2008.12.006
Csankovszki G, et al. Three Distinct Condensin Complexes Control C. Elegans Chromosome Dynamics. Curr Biol. 2009 Jan 13;19(1):9-19. PubMed PMID: 19119011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Three distinct condensin complexes control C. elegans chromosome dynamics. AU - Csankovszki,Gyorgyi, AU - Collette,Karishma, AU - Spahl,Karin, AU - Carey,James, AU - Snyder,Martha, AU - Petty,Emily, AU - Patel,Uchita, AU - Tabuchi,Tomoko, AU - Liu,Hongbin, AU - McLeod,Ian, AU - Thompson,James, AU - Sarkeshik,Ali, AU - Sarkesik,Ali, AU - Yates,John, AU - Meyer,Barbara J, AU - Hagstrom,Kirsten, PY - 2008/10/10/received PY - 2008/12/02/revised PY - 2008/12/04/accepted PY - 2009/1/3/entrez PY - 2009/1/3/pubmed PY - 2009/4/30/medline SP - 9 EP - 19 JF - Current biology : CB JO - Curr. Biol. VL - 19 IS - 1 N2 - BACKGROUND: Condensin complexes organize chromosome structure and facilitate chromosome segregation. Higher eukaryotes have two complexes, condensin I and condensin II, each essential for chromosome segregation. The nematode Caenorhabditis elegans was considered an exception, because it has a mitotic condensin II complex but appeared to lack mitotic condensin I. Instead, its condensin I-like complex (here called condensin I(DC)) dampens gene expression along hermaphrodite X chromosomes during dosage compensation. RESULTS: Here we report the discovery of a third condensin complex, condensin I, in C. elegans. We identify new condensin subunits and show that each complex has a conserved five-subunit composition. Condensin I differs from condensin I(DC) by only a single subunit. Yet condensin I binds to autosomes and X chromosomes in both sexes to promote chromosome segregation, whereas condensin I(DC) binds specifically to X chromosomes in hermaphrodites to regulate transcript levels. Both condensin I and II promote chromosome segregation, but associate with different chromosomal regions during mitosis and meiosis. Unexpectedly, condensin I also localizes to regions of cohesion between meiotic chromosomes before their segregation. CONCLUSIONS: We demonstrate that condensin subunits in C. elegans form three complexes, one that functions in dosage compensation and two that function in mitosis and meiosis. These results highlight how the duplication and divergence of condensin subunits during evolution may facilitate their adaptation to specialized chromosomal roles and illustrate the versatility of condensins to function in both gene regulation and chromosome segregation. SN - 1879-0445 UR - https://www.unboundmedicine.com/medline/citation/19119011/Three_distinct_condensin_complexes_control_C__elegans_chromosome_dynamics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-9822(08)01627-8 DB - PRIME DP - Unbound Medicine ER -