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Molecular identification and typing of Burkholderia pseudomallei and Burkholderia mallei: when is enough enough?
Trans R Soc Trop Med Hyg. 2008 Dec; 102 Suppl 1:S134-9.TR

Abstract

Burkholderia mallei and B. pseudomallei are highly pathogenic microorganisms for both humans and animals. Moreover, they are regarded as potential agents of bioterrorism. Thus, rapid and unequivocal detection and identification of these dangerous pathogens is critical. In the present study, we describe the use of an optimized protocol for the early diagnosis of experimental glanders and melioidosis and for the rapid differentiation and typing of Burkholderia strains. This experience with PCR-based identification methods indicates that single PCR targets (23S and 16S rRNA genes, 16S-23S intergenic region, fliC and type III secretion gene cluster) should be used with caution for identification of B. mallei and B. pseudomallei, and need to be used alongside molecular methods such as gene sequencing. Several molecular typing procedures have been used to identify genetically related B. pseudomallei and B. mallei isolates, including ribotyping, pulsed-field gel electrophoresis and multilocus sequence typing. However, these methods are time consuming and technically challenging for many laboratories. RAPD, variable amplicon typing scheme, Rep-PCR, BOX-PCR and multiple-locus variable-number tandem repeat analysis have been recommended by us for the rapid differentiation of B. mallei and B. pseudomallei strains.

Authors+Show Affiliations

Research Institute for Plague Control, Golubinskaya st. 7, 400131 Volgograd, Russia. antonovava@rambler.ruNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19121675

Citation

Antonov, Valery A., et al. "Molecular Identification and Typing of Burkholderia Pseudomallei and Burkholderia Mallei: when Is Enough Enough?" Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 102 Suppl 1, 2008, pp. S134-9.
Antonov VA, Tkachenko GA, Altukhova VV, et al. Molecular identification and typing of Burkholderia pseudomallei and Burkholderia mallei: when is enough enough? Trans R Soc Trop Med Hyg. 2008;102 Suppl 1:S134-9.
Antonov, V. A., Tkachenko, G. A., Altukhova, V. V., Savchenko, S. S., Zinchenko, O. V., Viktorov, D. V., Zamaraev, V. S., Ilyukhin, V. I., & Alekseev, V. V. (2008). Molecular identification and typing of Burkholderia pseudomallei and Burkholderia mallei: when is enough enough? Transactions of the Royal Society of Tropical Medicine and Hygiene, 102 Suppl 1, S134-9. https://doi.org/10.1016/S0035-9203(08)70030-0
Antonov VA, et al. Molecular Identification and Typing of Burkholderia Pseudomallei and Burkholderia Mallei: when Is Enough Enough. Trans R Soc Trop Med Hyg. 2008;102 Suppl 1:S134-9. PubMed PMID: 19121675.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular identification and typing of Burkholderia pseudomallei and Burkholderia mallei: when is enough enough? AU - Antonov,Valery A, AU - Tkachenko,Galina A, AU - Altukhova,Viktoriya V, AU - Savchenko,Sergey S, AU - Zinchenko,Olga V, AU - Viktorov,Dmitry V, AU - Zamaraev,Valery S, AU - Ilyukhin,Vladimir I, AU - Alekseev,Vladimir V, PY - 2009/1/6/entrez PY - 2009/1/16/pubmed PY - 2009/11/11/medline SP - S134 EP - 9 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans R Soc Trop Med Hyg VL - 102 Suppl 1 N2 - Burkholderia mallei and B. pseudomallei are highly pathogenic microorganisms for both humans and animals. Moreover, they are regarded as potential agents of bioterrorism. Thus, rapid and unequivocal detection and identification of these dangerous pathogens is critical. In the present study, we describe the use of an optimized protocol for the early diagnosis of experimental glanders and melioidosis and for the rapid differentiation and typing of Burkholderia strains. This experience with PCR-based identification methods indicates that single PCR targets (23S and 16S rRNA genes, 16S-23S intergenic region, fliC and type III secretion gene cluster) should be used with caution for identification of B. mallei and B. pseudomallei, and need to be used alongside molecular methods such as gene sequencing. Several molecular typing procedures have been used to identify genetically related B. pseudomallei and B. mallei isolates, including ribotyping, pulsed-field gel electrophoresis and multilocus sequence typing. However, these methods are time consuming and technically challenging for many laboratories. RAPD, variable amplicon typing scheme, Rep-PCR, BOX-PCR and multiple-locus variable-number tandem repeat analysis have been recommended by us for the rapid differentiation of B. mallei and B. pseudomallei strains. SN - 1878-3503 UR - https://www.unboundmedicine.com/medline/citation/19121675/Molecular_identification_and_typing_of_Burkholderia_pseudomallei_and_Burkholderia_mallei:_when_is_enough_enough L2 - https://linkinghub.elsevier.com/retrieve/pii/S0035-9203(08)70030-0 DB - PRIME DP - Unbound Medicine ER -