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The serine hydroxymethyltransferase gene glyA in Corynebacterium glutamicum is controlled by GlyR.
J Biotechnol. 2009 Feb 05; 139(3):214-21.JB

Abstract

Serine hydroxymethyltransferase (SHMT) occupies a central position in one-carbon metabolism, and we here study its regulation in Corynebacterium glutamicum. Enzyme quantifications revealed an about 3-fold increase of SHMT activity during exponential growth with a further increase at the onset of the stationary phase. The SHMT encoding glyA gene was shown to be transcribed as a monocistronic mRNA, and its transcriptional start site was determined. Using DNA affinity chromatography the regulator GlyR (Cg0527) was identified and its chromosomal deletion shown to abolish the increase in SHMT activity in the stationary phase. The involvement of GlyR in glyA control was further confirmed by a transcriptional fusion of the glyA promoter with cat and chloramphenicol acetyltransferase quantifications. GlyR was isolated and mutational studies together with electrophoretic mobility shift assays showed that it binds to the imperfect palindromic motif CACT-N(2)-AATG in the -119 to -96 upstream region of the glyA promoter. These and further data illustrate that the essential SHMT has highest activity in the stationary phase and that GlyR acts as an activator of glyA transcription in this growth phase.

Authors+Show Affiliations

Institut für Biotechnologie 1, Forschungszentrum Jülich, Jülich, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19124047

Citation

Schweitzer, Jens-Eric, et al. "The Serine Hydroxymethyltransferase Gene glyA in Corynebacterium Glutamicum Is Controlled By GlyR." Journal of Biotechnology, vol. 139, no. 3, 2009, pp. 214-21.
Schweitzer JE, Stolz M, Diesveld R, et al. The serine hydroxymethyltransferase gene glyA in Corynebacterium glutamicum is controlled by GlyR. J Biotechnol. 2009;139(3):214-21.
Schweitzer, J. E., Stolz, M., Diesveld, R., Etterich, H., & Eggeling, L. (2009). The serine hydroxymethyltransferase gene glyA in Corynebacterium glutamicum is controlled by GlyR. Journal of Biotechnology, 139(3), 214-21. https://doi.org/10.1016/j.jbiotec.2008.12.008
Schweitzer JE, et al. The Serine Hydroxymethyltransferase Gene glyA in Corynebacterium Glutamicum Is Controlled By GlyR. J Biotechnol. 2009 Feb 5;139(3):214-21. PubMed PMID: 19124047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The serine hydroxymethyltransferase gene glyA in Corynebacterium glutamicum is controlled by GlyR. AU - Schweitzer,Jens-Eric, AU - Stolz,Michael, AU - Diesveld,Ramon, AU - Etterich,Helga, AU - Eggeling,Lothar, Y1 - 2008/12/16/ PY - 2008/11/05/received PY - 2008/12/03/revised PY - 2008/12/09/accepted PY - 2009/1/7/entrez PY - 2009/1/7/pubmed PY - 2009/4/7/medline SP - 214 EP - 21 JF - Journal of biotechnology JO - J. Biotechnol. VL - 139 IS - 3 N2 - Serine hydroxymethyltransferase (SHMT) occupies a central position in one-carbon metabolism, and we here study its regulation in Corynebacterium glutamicum. Enzyme quantifications revealed an about 3-fold increase of SHMT activity during exponential growth with a further increase at the onset of the stationary phase. The SHMT encoding glyA gene was shown to be transcribed as a monocistronic mRNA, and its transcriptional start site was determined. Using DNA affinity chromatography the regulator GlyR (Cg0527) was identified and its chromosomal deletion shown to abolish the increase in SHMT activity in the stationary phase. The involvement of GlyR in glyA control was further confirmed by a transcriptional fusion of the glyA promoter with cat and chloramphenicol acetyltransferase quantifications. GlyR was isolated and mutational studies together with electrophoretic mobility shift assays showed that it binds to the imperfect palindromic motif CACT-N(2)-AATG in the -119 to -96 upstream region of the glyA promoter. These and further data illustrate that the essential SHMT has highest activity in the stationary phase and that GlyR acts as an activator of glyA transcription in this growth phase. SN - 0168-1656 UR - https://www.unboundmedicine.com/medline/citation/19124047/The_serine_hydroxymethyltransferase_gene_glyA_in_Corynebacterium_glutamicum_is_controlled_by_GlyR_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1656(08)02477-2 DB - PRIME DP - Unbound Medicine ER -