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Development of an electrochemical immunoassay for rapid detection of E. coli using anodic stripping voltammetry based on Cu@Au nanoparticles as antibody labels.
Biosens Bioelectron. 2009 Mar 15; 24(7):2155-9.BB

Abstract

A sensitive electrochemical immunoassay for rapid detection of Escherichia coli has been developed by anodic stripping voltammetry (ASV) based on core-shell Cu@Au nanoparticles (NPs) as anti-E. coli antibody labels. The characteristics of Cu@Au NPs before and after binding with antibody were confirmed by transmission electron microscopy (TEM). After Cu@Au-labeled antibody reacted with the immobilized E. coli on Polystyrene (PS)-modified ITO chip, Cu@Au NPs were dissolved by oxidation to the metal ionic forms, and the released Cu(2+) ions were determined at GC/Nafion/Hg modified electrode by ASV. The utilization of GC/Nafion/Hg modified electrode could enhance the sensitivity for Cu(2+) detection with a concentration as low as 9.0 x 10(-12)mol/L. Since Cu@Au NPs labels were only present when antibody reacted with E. coli, the amount of Cu(2+) directly reflected the number of E. coli. The technique could detect E. coli with a detection limit of 30CFU/mL and the overall analysis could be completed in 2h. By introducing a pre-enrichment step, a concentration of 3CFU/10mL E. coli in surface water was detected by the electrochemical immunoassay.

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Authors+Show Affiliations

Zhang X
Department of Chemistry, East China Normal University, Shanghai 200062, PR China.
Geng P
No affiliation info available
Liu H
No affiliation info available
Teng Y
No affiliation info available
Liu Y
No affiliation info available
Wang Q
No affiliation info available
Zhang W
No affiliation info available
Jin L
No affiliation info available
Jiang L
No affiliation info available

MeSH

Biosensing TechniquesCarbonColony Count, MicrobialElectrodesEquipment DesignEquipment Failure AnalysisEscherichia coliGoldImmunoassayNanoparticlesReproducibility of ResultsSensitivity and SpecificityStaining and Labeling

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19124236

Citation

Zhang, Xinai, et al. "Development of an Electrochemical Immunoassay for Rapid Detection of E. Coli Using Anodic Stripping Voltammetry Based On Cu@Au Nanoparticles as Antibody Labels." Biosensors & Bioelectronics, vol. 24, no. 7, 2009, pp. 2155-9.
Zhang X, Geng P, Liu H, et al. Development of an electrochemical immunoassay for rapid detection of E. coli using anodic stripping voltammetry based on Cu@Au nanoparticles as antibody labels. Biosens Bioelectron. 2009;24(7):2155-9.
Zhang, X., Geng, P., Liu, H., Teng, Y., Liu, Y., Wang, Q., Zhang, W., Jin, L., & Jiang, L. (2009). Development of an electrochemical immunoassay for rapid detection of E. coli using anodic stripping voltammetry based on Cu@Au nanoparticles as antibody labels. Biosensors & Bioelectronics, 24(7), 2155-9. https://doi.org/10.1016/j.bios.2008.11.019
Zhang X, et al. Development of an Electrochemical Immunoassay for Rapid Detection of E. Coli Using Anodic Stripping Voltammetry Based On Cu@Au Nanoparticles as Antibody Labels. Biosens Bioelectron. 2009 Mar 15;24(7):2155-9. PubMed PMID: 19124236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of an electrochemical immunoassay for rapid detection of E. coli using anodic stripping voltammetry based on Cu@Au nanoparticles as antibody labels. AU - Zhang,Xinai, AU - Geng,Ping, AU - Liu,Huijie, AU - Teng,Yingqiao, AU - Liu,Yajun, AU - Wang,Qingjiang, AU - Zhang,Wen, AU - Jin,Litong, AU - Jiang,Lai, Y1 - 2008/12/03/ PY - 2008/09/20/received PY - 2008/11/12/revised PY - 2008/11/17/accepted PY - 2009/1/7/entrez PY - 2009/1/7/pubmed PY - 2009/5/27/medline SP - 2155 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 7 N2 - A sensitive electrochemical immunoassay for rapid detection of Escherichia coli has been developed by anodic stripping voltammetry (ASV) based on core-shell Cu@Au nanoparticles (NPs) as anti-E. coli antibody labels. The characteristics of Cu@Au NPs before and after binding with antibody were confirmed by transmission electron microscopy (TEM). After Cu@Au-labeled antibody reacted with the immobilized E. coli on Polystyrene (PS)-modified ITO chip, Cu@Au NPs were dissolved by oxidation to the metal ionic forms, and the released Cu(2+) ions were determined at GC/Nafion/Hg modified electrode by ASV. The utilization of GC/Nafion/Hg modified electrode could enhance the sensitivity for Cu(2+) detection with a concentration as low as 9.0 x 10(-12)mol/L. Since Cu@Au NPs labels were only present when antibody reacted with E. coli, the amount of Cu(2+) directly reflected the number of E. coli. The technique could detect E. coli with a detection limit of 30CFU/mL and the overall analysis could be completed in 2h. By introducing a pre-enrichment step, a concentration of 3CFU/10mL E. coli in surface water was detected by the electrochemical immunoassay. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19124236/Development_of_an_electrochemical_immunoassay_for_rapid_detection_of_E__coli_using_anodic_stripping_voltammetry_based_on_Cu@Au_nanoparticles_as_antibody_labels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00622-2 DB - PRIME DP - Unbound Medicine ER -