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Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray.
Bioprocess Biosyst Eng. 2009 Aug; 32(5):681-8.BB

Abstract

In industrial process, yeast cells are exposed to ethanol stress that affects the cell growth and the productivity. Thus, investigating the intracellular state of yeast cells under high ethanol concentration is important. In this study, using DNA microarray analysis, we performed comprehensive expression profiling of two strains of Saccharomyces cerevisiae, i.e., the ethanol-adapted strain that shows active growth under the ethanol stress condition and its parental strain used as the control. By comparing the expression profiles of these two strains under the ethanol stress condition, we found that the genes related to ribosomal proteins were highly up-regulated in the ethanol-adapted strain. Further, genes related to ATP synthesis in mitochondria were suggested to be important for growth under ethanol stress. We expect that the results will provide a better understanding of ethanol tolerance of yeast.

Authors+Show Affiliations

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19125301

Citation

Dinh, Thai Nho, et al. "Analysis of Adaptation to High Ethanol Concentration in Saccharomyces Cerevisiae Using DNA Microarray." Bioprocess and Biosystems Engineering, vol. 32, no. 5, 2009, pp. 681-8.
Dinh TN, Nagahisa K, Yoshikawa K, et al. Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray. Bioprocess Biosyst Eng. 2009;32(5):681-8.
Dinh, T. N., Nagahisa, K., Yoshikawa, K., Hirasawa, T., Furusawa, C., & Shimizu, H. (2009). Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray. Bioprocess and Biosystems Engineering, 32(5), 681-8. https://doi.org/10.1007/s00449-008-0292-7
Dinh TN, et al. Analysis of Adaptation to High Ethanol Concentration in Saccharomyces Cerevisiae Using DNA Microarray. Bioprocess Biosyst Eng. 2009;32(5):681-8. PubMed PMID: 19125301.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray. AU - Dinh,Thai Nho, AU - Nagahisa,Keisuke, AU - Yoshikawa,Katsunori, AU - Hirasawa,Takashi, AU - Furusawa,Chikara, AU - Shimizu,Hiroshi, Y1 - 2009/01/06/ PY - 2008/12/11/received PY - 2008/12/17/accepted PY - 2009/1/7/entrez PY - 2009/1/7/pubmed PY - 2009/10/8/medline SP - 681 EP - 8 JF - Bioprocess and biosystems engineering JO - Bioprocess Biosyst Eng VL - 32 IS - 5 N2 - In industrial process, yeast cells are exposed to ethanol stress that affects the cell growth and the productivity. Thus, investigating the intracellular state of yeast cells under high ethanol concentration is important. In this study, using DNA microarray analysis, we performed comprehensive expression profiling of two strains of Saccharomyces cerevisiae, i.e., the ethanol-adapted strain that shows active growth under the ethanol stress condition and its parental strain used as the control. By comparing the expression profiles of these two strains under the ethanol stress condition, we found that the genes related to ribosomal proteins were highly up-regulated in the ethanol-adapted strain. Further, genes related to ATP synthesis in mitochondria were suggested to be important for growth under ethanol stress. We expect that the results will provide a better understanding of ethanol tolerance of yeast. SN - 1615-7605 UR - https://www.unboundmedicine.com/medline/citation/19125301/Analysis_of_adaptation_to_high_ethanol_concentration_in_Saccharomyces_cerevisiae_using_DNA_microarray_ L2 - https://dx.doi.org/10.1007/s00449-008-0292-7 DB - PRIME DP - Unbound Medicine ER -