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Enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence testing in a bullous pemphigoid and pemphigoid gestationis.
Int J Dermatol. 2008 Dec; 47(12):1245-9.IJ

Abstract

Enzyme-linked immunosorbent assay (ELISA) is an excellent tool for detection of circulating antibodies against the NC16A portion of BP180 antigen. We compared the sensitivity and specificity of a commercially available BP180-NC16a domain ELISA with that of an indirect immunofluorescence (IIF) testing in the evaluation of bullous pemphigoid (BP) and pemphigoid gestationis (PG), and analyzed the relationship between ELISA results and the presence of IgG deposition, in an epidermal or combined pattern, on direct immunofluorescence (DIF) testing of salt-split skin. ELISA was performed on serum from 28 patients (24 BP, 4 PG) and 50 controls. IIF testing was performed on serum from 27 patients and 98 controls. For the group of 28 patients with BP or PG, ELISA had a sensitivity of 93% and specificity of 96% (P < 0.001), while sensitivity was 74% and specificity 96% (P < 0.001) for IIF testing. In these patients, ELISA has a higher sensitivity than IIF testing, but similar specificity. Evaluation of controls who had IgG deposition on the dermal side of salt-split skin on DIF testing showed specificity for the ELISA of 100% (all four cases negative) and 80% for IIF testing (one of five positive). Positive ELISA correlated with a diagnosis of BP or PG only in patients who had IgG at the basement membrane zone (BMZ) by DIF testing. Overall, ELISA appears to have greater sensitivity and specificity for BP or PG than does IIF testing.

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Authors+Show Affiliations

Barnadas MA
Department of Dermatology, Hospital Sta. Creu i St. Pau, Barcelona, Spain. mbarnadas@hsp.santpau.es
Rubiales MV
No affiliation info available
González MJ
No affiliation info available
Puig L
No affiliation info available
García P
No affiliation info available
Baselga E
No affiliation info available
Pujol R
No affiliation info available
Alomar A
No affiliation info available
Gelpí C
No affiliation info available

MeSH

AdolescentAdultAgedAutoantibodiesAutoantigensChildChild, PreschoolEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody Technique, IndirectHomeodomain ProteinsHumansImmunoglobulin GInfantInfant, NewbornMaleMiddle AgedNon-Fibrillar CollagensPemphigoid GestationisPemphigoid, BullousPredictive Value of TestsPregnancySensitivity and SpecificitySkinTranscription FactorsYoung Adult

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19126009

Citation

Barnadas, María A., et al. "Enzyme-linked Immunosorbent Assay (ELISA) and Indirect Immunofluorescence Testing in a Bullous Pemphigoid and Pemphigoid Gestationis." International Journal of Dermatology, vol. 47, no. 12, 2008, pp. 1245-9.
Barnadas MA, Rubiales MV, González MJ, et al. Enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence testing in a bullous pemphigoid and pemphigoid gestationis. Int J Dermatol. 2008;47(12):1245-9.
Barnadas, M. A., Rubiales, M. V., González, M. J., Puig, L., García, P., Baselga, E., Pujol, R., Alomar, A., & Gelpí, C. (2008). Enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence testing in a bullous pemphigoid and pemphigoid gestationis. International Journal of Dermatology, 47(12), 1245-9. https://doi.org/10.1111/j.1365-4632.2008.03824.x
Barnadas MA, et al. Enzyme-linked Immunosorbent Assay (ELISA) and Indirect Immunofluorescence Testing in a Bullous Pemphigoid and Pemphigoid Gestationis. Int J Dermatol. 2008;47(12):1245-9. PubMed PMID: 19126009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence testing in a bullous pemphigoid and pemphigoid gestationis. AU - Barnadas,María A, AU - Rubiales,M Victòria, AU - González,M José, AU - Puig,Luis, AU - García,Pilar, AU - Baselga,Eulàlia, AU - Pujol,Ramón, AU - Alomar,Agustín, AU - Gelpí,Carmen, PY - 2009/1/8/entrez PY - 2009/1/8/pubmed PY - 2009/4/30/medline SP - 1245 EP - 9 JF - International journal of dermatology JO - Int J Dermatol VL - 47 IS - 12 N2 - Enzyme-linked immunosorbent assay (ELISA) is an excellent tool for detection of circulating antibodies against the NC16A portion of BP180 antigen. We compared the sensitivity and specificity of a commercially available BP180-NC16a domain ELISA with that of an indirect immunofluorescence (IIF) testing in the evaluation of bullous pemphigoid (BP) and pemphigoid gestationis (PG), and analyzed the relationship between ELISA results and the presence of IgG deposition, in an epidermal or combined pattern, on direct immunofluorescence (DIF) testing of salt-split skin. ELISA was performed on serum from 28 patients (24 BP, 4 PG) and 50 controls. IIF testing was performed on serum from 27 patients and 98 controls. For the group of 28 patients with BP or PG, ELISA had a sensitivity of 93% and specificity of 96% (P < 0.001), while sensitivity was 74% and specificity 96% (P < 0.001) for IIF testing. In these patients, ELISA has a higher sensitivity than IIF testing, but similar specificity. Evaluation of controls who had IgG deposition on the dermal side of salt-split skin on DIF testing showed specificity for the ELISA of 100% (all four cases negative) and 80% for IIF testing (one of five positive). Positive ELISA correlated with a diagnosis of BP or PG only in patients who had IgG at the basement membrane zone (BMZ) by DIF testing. Overall, ELISA appears to have greater sensitivity and specificity for BP or PG than does IIF testing. SN - 1365-4632 UR - https://www.unboundmedicine.com/medline/citation/19126009/Enzyme_linked_immunosorbent_assay__ELISA__and_indirect_immunofluorescence_testing_in_a_bullous_pemphigoid_and_pemphigoid_gestationis_ L2 - https://doi.org/10.1111/j.1365-4632.2008.03824.x DB - PRIME DP - Unbound Medicine ER -