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Genetic analysis of antimicrobial resistance in Escherichia coli isolated from diarrheic neonatal calves.
Vet Microbiol 2009; 136(3-4):397-402VM

Abstract

This study was carried out to screen and analyze the genetic basis of antimicrobial resistance in Escherichia coli strains isolated from neonatal calf diarrhea in Egypt. A total of 182 isolates of E. coli recovered from 91 diarrheic neonatal calves were analyzed for antimicrobial susceptibilities, the presence of class 1 and class 2 integrons and antimicrobial resistance genes. Nineteen isolates (10.4%) showed multidrug resistance phenotypes and harbored at least three antimicrobial resistance genes. PCR screening detected class 1 integrons in 19 isolates (10.4%) and class 2 integrons in 2 isolates (1.1%). The identified antimicrobial resistance genes within class 1 integrons were dihydrofolate reductase types: dfrA1, dfrA12, dfrA15 and dfrA17, which confer resistance to trimethoprim; aminoglycoside adenyltransferase types: aadA1, aadA2, aadA5, aadA7 and aadA23, which confer resistance to streptomycin and spectinomycin; and aminoglycoside acetyltransferase gene, aac(3)-Id, which confers resistance to gentamicin and sisomicin. Furthermore, many beta-lactamases encoding genes, plasmid-mediated quinolone resistance genes and florfenicol resistance gene were identified in this study. To the best of our knowledge, this is the first report for molecular characterization of antimicrobial resistance in E. coli isolated from diarrheic neonatal calves in Africa.

Authors+Show Affiliations

Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh 33516, Egypt. ashrafa5@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19128900

Citation

Ahmed, Ashraf M., et al. "Genetic Analysis of Antimicrobial Resistance in Escherichia Coli Isolated From Diarrheic Neonatal Calves." Veterinary Microbiology, vol. 136, no. 3-4, 2009, pp. 397-402.
Ahmed AM, Younis EE, Osman SA, et al. Genetic analysis of antimicrobial resistance in Escherichia coli isolated from diarrheic neonatal calves. Vet Microbiol. 2009;136(3-4):397-402.
Ahmed, A. M., Younis, E. E., Osman, S. A., Ishida, Y., El-Khodery, S. A., & Shimamoto, T. (2009). Genetic analysis of antimicrobial resistance in Escherichia coli isolated from diarrheic neonatal calves. Veterinary Microbiology, 136(3-4), pp. 397-402. doi:10.1016/j.vetmic.2008.11.021.
Ahmed AM, et al. Genetic Analysis of Antimicrobial Resistance in Escherichia Coli Isolated From Diarrheic Neonatal Calves. Vet Microbiol. 2009 May 12;136(3-4):397-402. PubMed PMID: 19128900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic analysis of antimicrobial resistance in Escherichia coli isolated from diarrheic neonatal calves. AU - Ahmed,Ashraf M, AU - Younis,Emad E A, AU - Osman,Salama A, AU - Ishida,Yojiro, AU - El-Khodery,Sabry A, AU - Shimamoto,Tadashi, Y1 - 2008/12/03/ PY - 2008/08/29/received PY - 2008/11/26/revised PY - 2008/11/28/accepted PY - 2009/1/9/entrez PY - 2009/1/9/pubmed PY - 2009/8/6/medline SP - 397 EP - 402 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 136 IS - 3-4 N2 - This study was carried out to screen and analyze the genetic basis of antimicrobial resistance in Escherichia coli strains isolated from neonatal calf diarrhea in Egypt. A total of 182 isolates of E. coli recovered from 91 diarrheic neonatal calves were analyzed for antimicrobial susceptibilities, the presence of class 1 and class 2 integrons and antimicrobial resistance genes. Nineteen isolates (10.4%) showed multidrug resistance phenotypes and harbored at least three antimicrobial resistance genes. PCR screening detected class 1 integrons in 19 isolates (10.4%) and class 2 integrons in 2 isolates (1.1%). The identified antimicrobial resistance genes within class 1 integrons were dihydrofolate reductase types: dfrA1, dfrA12, dfrA15 and dfrA17, which confer resistance to trimethoprim; aminoglycoside adenyltransferase types: aadA1, aadA2, aadA5, aadA7 and aadA23, which confer resistance to streptomycin and spectinomycin; and aminoglycoside acetyltransferase gene, aac(3)-Id, which confers resistance to gentamicin and sisomicin. Furthermore, many beta-lactamases encoding genes, plasmid-mediated quinolone resistance genes and florfenicol resistance gene were identified in this study. To the best of our knowledge, this is the first report for molecular characterization of antimicrobial resistance in E. coli isolated from diarrheic neonatal calves in Africa. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/19128900/Genetic_analysis_of_antimicrobial_resistance_in_Escherichia_coli_isolated_from_diarrheic_neonatal_calves_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(08)00549-X DB - PRIME DP - Unbound Medicine ER -