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Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae.
Bioresour Technol. 2009 Apr; 100(8):2392-8.BT

Abstract

In this study, five recombinant Saccharomyces cerevisiae strains were compared for their xylose-fermenting ability. The most efficient xylose-to-ethanol fermentation was found by using the industrial strain MA-R4, in which the genes for xylose reductase and xylitol dehydrogenase from Pichia stipitis along with an endogenous xylulokinase gene were expressed by chromosomal integration of the flocculent yeast strain IR-2. The MA-R4 strain rapidly converted xylose to ethanol with a low xylitol yield. Furthermore, the MA-R4 strain had the highest ethanol production when fermenting not only a mixture of glucose and xylose, but also mixed sugars in the detoxified hydrolysate of wood chips. These results collectively suggest that MA-R4 may be a suitable recombinant strain for further study into large-scale ethanol production from mixed sugars present in lignocellulosic hydrolysates.

Authors+Show Affiliations

Biomass Technology Research Center (BTRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan. a-matsushika@aist.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19128960

Citation

Matsushika, Akinori, et al. "Bioethanol Production Performance of Five Recombinant Strains of Laboratory and Industrial Xylose-fermenting Saccharomyces Cerevisiae." Bioresource Technology, vol. 100, no. 8, 2009, pp. 2392-8.
Matsushika A, Inoue H, Murakami K, et al. Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae. Bioresour Technol. 2009;100(8):2392-8.
Matsushika, A., Inoue, H., Murakami, K., Takimura, O., & Sawayama, S. (2009). Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae. Bioresource Technology, 100(8), 2392-8. https://doi.org/10.1016/j.biortech.2008.11.047
Matsushika A, et al. Bioethanol Production Performance of Five Recombinant Strains of Laboratory and Industrial Xylose-fermenting Saccharomyces Cerevisiae. Bioresour Technol. 2009;100(8):2392-8. PubMed PMID: 19128960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae. AU - Matsushika,Akinori, AU - Inoue,Hiroyuki, AU - Murakami,Katsuji, AU - Takimura,Osamu, AU - Sawayama,Shigeki, Y1 - 2009/01/06/ PY - 2008/08/24/received PY - 2008/11/26/revised PY - 2008/11/26/accepted PY - 2009/1/9/entrez PY - 2009/1/9/pubmed PY - 2009/3/7/medline SP - 2392 EP - 8 JF - Bioresource technology JO - Bioresour Technol VL - 100 IS - 8 N2 - In this study, five recombinant Saccharomyces cerevisiae strains were compared for their xylose-fermenting ability. The most efficient xylose-to-ethanol fermentation was found by using the industrial strain MA-R4, in which the genes for xylose reductase and xylitol dehydrogenase from Pichia stipitis along with an endogenous xylulokinase gene were expressed by chromosomal integration of the flocculent yeast strain IR-2. The MA-R4 strain rapidly converted xylose to ethanol with a low xylitol yield. Furthermore, the MA-R4 strain had the highest ethanol production when fermenting not only a mixture of glucose and xylose, but also mixed sugars in the detoxified hydrolysate of wood chips. These results collectively suggest that MA-R4 may be a suitable recombinant strain for further study into large-scale ethanol production from mixed sugars present in lignocellulosic hydrolysates. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/19128960/Bioethanol_production_performance_of_five_recombinant_strains_of_laboratory_and_industrial_xylose_fermenting_Saccharomyces_cerevisiae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(08)01025-0 DB - PRIME DP - Unbound Medicine ER -