TY - JOUR T1 - Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae. AU - Matsushika,Akinori, AU - Inoue,Hiroyuki, AU - Murakami,Katsuji, AU - Takimura,Osamu, AU - Sawayama,Shigeki, Y1 - 2009/01/06/ PY - 2008/08/24/received PY - 2008/11/26/revised PY - 2008/11/26/accepted PY - 2009/1/9/entrez PY - 2009/1/9/pubmed PY - 2009/3/7/medline SP - 2392 EP - 8 JF - Bioresource technology JO - Bioresour Technol VL - 100 IS - 8 N2 - In this study, five recombinant Saccharomyces cerevisiae strains were compared for their xylose-fermenting ability. The most efficient xylose-to-ethanol fermentation was found by using the industrial strain MA-R4, in which the genes for xylose reductase and xylitol dehydrogenase from Pichia stipitis along with an endogenous xylulokinase gene were expressed by chromosomal integration of the flocculent yeast strain IR-2. The MA-R4 strain rapidly converted xylose to ethanol with a low xylitol yield. Furthermore, the MA-R4 strain had the highest ethanol production when fermenting not only a mixture of glucose and xylose, but also mixed sugars in the detoxified hydrolysate of wood chips. These results collectively suggest that MA-R4 may be a suitable recombinant strain for further study into large-scale ethanol production from mixed sugars present in lignocellulosic hydrolysates. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/19128960/Bioethanol_production_performance_of_five_recombinant_strains_of_laboratory_and_industrial_xylose_fermenting_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -