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Salivary gland transcriptome analysis during Plasmodium infection in malaria vector Anopheles stephensi.
Int J Infect Dis 2009; 13(5):636-46IJ

Abstract

BACKGROUND

Understanding the tissue-specific molecular cross-talk mechanism during the mosquito-parasite interaction is of prime importance in the design of new strategies for malaria control. Because mosquito salivary glands are the final destination for the parasite maturation and transmission of vector-borne diseases, identification and characterization of salivary genes and their products are equally important in order to access their effect on the infectivity of the parasite. During the last five years there have been several studies on the sialomes of Anopheles mosquitoes, however very limited information is available on the changes in the salivary gland transcriptome in the presence of Plasmodium, and this information is limited to the mosquito Anopheles gambiae.

METHODS

In this study we aimed to explore and identify parasite-induced transcripts from the salivary glands of Anopheles stephensi, using a subtractive hybridization protocol.

RESULTS

Ninety-four percent of expressed sequence tags (ESTs) showed close homology to previously known families of mosquito salivary gland secretary proteins, representing the induced expression of alternative splicing and/or additional new members of the protein family. The remaining 6% of ESTs did not yield significant homology to any known proteins in the non-redundant database and thus may represent a class of unknown/novel salivary proteins. Primary analysis of the ESTs also revealed identification of several novel immune-related transcripts, including defensin and cecropins, probably involved in counter-activation of the antagonistic defense system. A comprehensive description of each family of proteins has been discussed in relation to the tissue-specific mosquito-parasite interaction.

CONCLUSION

This is the first report on the identification of new putative salivary genes, presumably activated during parasite infection.

Authors+Show Affiliations

Molecular Biology Unit, National Center for Cell Science, Ganeshkhind, Pune, India. dixit2k@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19128996

Citation

Dixit, Rajnikant, et al. "Salivary Gland Transcriptome Analysis During Plasmodium Infection in Malaria Vector Anopheles Stephensi." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 13, no. 5, 2009, pp. 636-46.
Dixit R, Sharma A, Mourya DT, et al. Salivary gland transcriptome analysis during Plasmodium infection in malaria vector Anopheles stephensi. Int J Infect Dis. 2009;13(5):636-46.
Dixit, R., Sharma, A., Mourya, D. T., Kamaraju, R., Patole, M. S., & Shouche, Y. S. (2009). Salivary gland transcriptome analysis during Plasmodium infection in malaria vector Anopheles stephensi. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 13(5), pp. 636-46. doi:10.1016/j.ijid.2008.07.027.
Dixit R, et al. Salivary Gland Transcriptome Analysis During Plasmodium Infection in Malaria Vector Anopheles Stephensi. Int J Infect Dis. 2009;13(5):636-46. PubMed PMID: 19128996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salivary gland transcriptome analysis during Plasmodium infection in malaria vector Anopheles stephensi. AU - Dixit,Rajnikant, AU - Sharma,Arun, AU - Mourya,Devendra T, AU - Kamaraju,Raghavendra, AU - Patole,Millind S, AU - Shouche,Yogesh S, Y1 - 2009/01/06/ PY - 2008/01/14/received PY - 2008/06/19/revised PY - 2008/07/12/accepted PY - 2009/1/9/entrez PY - 2009/1/9/pubmed PY - 2009/12/16/medline SP - 636 EP - 46 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int. J. Infect. Dis. VL - 13 IS - 5 N2 - BACKGROUND: Understanding the tissue-specific molecular cross-talk mechanism during the mosquito-parasite interaction is of prime importance in the design of new strategies for malaria control. Because mosquito salivary glands are the final destination for the parasite maturation and transmission of vector-borne diseases, identification and characterization of salivary genes and their products are equally important in order to access their effect on the infectivity of the parasite. During the last five years there have been several studies on the sialomes of Anopheles mosquitoes, however very limited information is available on the changes in the salivary gland transcriptome in the presence of Plasmodium, and this information is limited to the mosquito Anopheles gambiae. METHODS: In this study we aimed to explore and identify parasite-induced transcripts from the salivary glands of Anopheles stephensi, using a subtractive hybridization protocol. RESULTS: Ninety-four percent of expressed sequence tags (ESTs) showed close homology to previously known families of mosquito salivary gland secretary proteins, representing the induced expression of alternative splicing and/or additional new members of the protein family. The remaining 6% of ESTs did not yield significant homology to any known proteins in the non-redundant database and thus may represent a class of unknown/novel salivary proteins. Primary analysis of the ESTs also revealed identification of several novel immune-related transcripts, including defensin and cecropins, probably involved in counter-activation of the antagonistic defense system. A comprehensive description of each family of proteins has been discussed in relation to the tissue-specific mosquito-parasite interaction. CONCLUSION: This is the first report on the identification of new putative salivary genes, presumably activated during parasite infection. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/19128996/Salivary_gland_transcriptome_analysis_during_Plasmodium_infection_in_malaria_vector_Anopheles_stephensi_ L2 - http://linkinghub.elsevier.com/retrieve/pii/S1201-9712(08)01693-7 DB - PRIME DP - Unbound Medicine ER -