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Constitutive expression of OsGH3.1 reduces auxin content and enhances defense response and resistance to a fungal pathogen in rice.
Mol Plant Microbe Interact. 2009 Feb; 22(2):201-10.MP

Abstract

GH3 genes are main components of the hormonal mechanism regulating growth and development and, hence, are deeply involved in a broad range of physiological processes. They are implicated in hormonal homeostasis through the conjugation to amino acids of the free form of essential plant growth regulators such as indoleacetic and jasmonic acids. In this work, we showed that OsGH3.1 overexpression in rice caused dwarfism and significantly reduced both free auxin content and cell elongation. Functional classification of the transcriptomic profiling revealed that most genes involved in auxin biosynthesis and auxin signaling inhibition were induced and repressed, respectively. Many genes related to cell organization and biogenesis were also significantly downregulated. The survey also showed that, although the response to abiotic stresses was not clearly stimulated, OsGH3.1 overexpression did activate a significant number of defense-related genes. In successive bioassays, it was demonstrated that the resistance of rice plants to pathogen infection, evaluated with two different Magnaporthe grisea strains, was higher in the transformants overexpressing OsGH3.1. Taken together, these results indicate that OsGH3.1 overexpression reduces auxin content, inhibits cell growth and cell wall loosening, and enhances resistance to a fungal pathogen. Our results provide evidence that auxin homeostasis can regulate the activation of the defense response in rice.

Authors+Show Affiliations

Instituto Valenciano de Investigaciones Agrarias. Carretera Moncada-Naquera, Moncada, Spain. Domingo_concar@gva.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19132872

Citation

Domingo, Concha, et al. "Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in Rice." Molecular Plant-microbe Interactions : MPMI, vol. 22, no. 2, 2009, pp. 201-10.
Domingo C, Andrés F, Tharreau D, et al. Constitutive expression of OsGH3.1 reduces auxin content and enhances defense response and resistance to a fungal pathogen in rice. Mol Plant Microbe Interact. 2009;22(2):201-10.
Domingo, C., Andrés, F., Tharreau, D., Iglesias, D. J., & Talón, M. (2009). Constitutive expression of OsGH3.1 reduces auxin content and enhances defense response and resistance to a fungal pathogen in rice. Molecular Plant-microbe Interactions : MPMI, 22(2), 201-10. https://doi.org/10.1094/MPMI-22-2-0201
Domingo C, et al. Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in Rice. Mol Plant Microbe Interact. 2009;22(2):201-10. PubMed PMID: 19132872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Constitutive expression of OsGH3.1 reduces auxin content and enhances defense response and resistance to a fungal pathogen in rice. AU - Domingo,Concha, AU - Andrés,Fernando, AU - Tharreau,Didier, AU - Iglesias,Domingo J, AU - Talón,Manuel, PY - 2009/1/10/entrez PY - 2009/1/10/pubmed PY - 2009/5/28/medline SP - 201 EP - 10 JF - Molecular plant-microbe interactions : MPMI JO - Mol. Plant Microbe Interact. VL - 22 IS - 2 N2 - GH3 genes are main components of the hormonal mechanism regulating growth and development and, hence, are deeply involved in a broad range of physiological processes. They are implicated in hormonal homeostasis through the conjugation to amino acids of the free form of essential plant growth regulators such as indoleacetic and jasmonic acids. In this work, we showed that OsGH3.1 overexpression in rice caused dwarfism and significantly reduced both free auxin content and cell elongation. Functional classification of the transcriptomic profiling revealed that most genes involved in auxin biosynthesis and auxin signaling inhibition were induced and repressed, respectively. Many genes related to cell organization and biogenesis were also significantly downregulated. The survey also showed that, although the response to abiotic stresses was not clearly stimulated, OsGH3.1 overexpression did activate a significant number of defense-related genes. In successive bioassays, it was demonstrated that the resistance of rice plants to pathogen infection, evaluated with two different Magnaporthe grisea strains, was higher in the transformants overexpressing OsGH3.1. Taken together, these results indicate that OsGH3.1 overexpression reduces auxin content, inhibits cell growth and cell wall loosening, and enhances resistance to a fungal pathogen. Our results provide evidence that auxin homeostasis can regulate the activation of the defense response in rice. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/19132872/Constitutive_expression_of_OsGH3_1_reduces_auxin_content_and_enhances_defense_response_and_resistance_to_a_fungal_pathogen_in_rice_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/MPMI-22-2-0201?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -