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The neuroprotective effects of NGF combined with GM1 on injured spinal cord neurons in vitro.
Brain Res Bull. 2009 Apr 06; 79(1):85-8.BR

Abstract

Monosialoganglioside (GM1) has been considered to have a neurotrophic factor-like activity. Nerve growth factor (NGF), a member of the neurotrophin family, is essential for neuronal survival, differentiation and maturation. The aim of the present study was to investigate whether co-administration of GM1 and NGF reverses glutamate (Glu) neurotoxicity in primary cultured rat embryonic spinal cord neurons. Spinal cord neurons were exposed to Glu (2 mmol/l), Glu (2 mmol/l) plus GM1 (10 mg/ml), Glu (2 mmol/l) plus NGF (10 ng/ml), Glu (2 mmol/l) plus GM1 (5 mg/ml) and NGF (5 ng/ml) and then processed for detecting intracellular concentrations of Ca2+([Ca2+]i) by confocal laser scanning microscopy and growth associated protein 43 (GAP43) mRNA by RT-PCR. The fluorescent intensity in Glu plus GM1 and NGF incubated neurons was the lowest as compared with that in other groups. The expression of GAP43 mRNA in Glu plus GM1 and NGF incubated neurons was the highest as compared with that in other groups. These results implicated that GM1 and NGF have synergistic neuroprotective effects on spinal cord neurons with excitotoxicity induced by Glu in vitro.

Authors+Show Affiliations

Department of Human Anatomy, Binzhou Medical College, No. 346 Guanhai Road, Yantai 264003, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19133317

Citation

Huang, Fei, et al. "The Neuroprotective Effects of NGF Combined With GM1 On Injured Spinal Cord Neurons in Vitro." Brain Research Bulletin, vol. 79, no. 1, 2009, pp. 85-8.
Huang F, Dong X, Zhang L, et al. The neuroprotective effects of NGF combined with GM1 on injured spinal cord neurons in vitro. Brain Res Bull. 2009;79(1):85-8.
Huang, F., Dong, X., Zhang, L., Zhang, X., Zhao, D., Bai, X., & Li, Z. (2009). The neuroprotective effects of NGF combined with GM1 on injured spinal cord neurons in vitro. Brain Research Bulletin, 79(1), 85-8. https://doi.org/10.1016/j.brainresbull.2008.12.007
Huang F, et al. The Neuroprotective Effects of NGF Combined With GM1 On Injured Spinal Cord Neurons in Vitro. Brain Res Bull. 2009 Apr 6;79(1):85-8. PubMed PMID: 19133317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The neuroprotective effects of NGF combined with GM1 on injured spinal cord neurons in vitro. AU - Huang,Fei, AU - Dong,Xifeng, AU - Zhang,Luping, AU - Zhang,Xuejun, AU - Zhao,Dongmei, AU - Bai,Xianyong, AU - Li,Zhenzhong, Y1 - 2009/01/06/ PY - 2008/11/01/received PY - 2008/12/03/revised PY - 2008/12/05/accepted PY - 2009/1/10/entrez PY - 2009/1/10/pubmed PY - 2009/5/13/medline SP - 85 EP - 8 JF - Brain research bulletin JO - Brain Res. Bull. VL - 79 IS - 1 N2 - Monosialoganglioside (GM1) has been considered to have a neurotrophic factor-like activity. Nerve growth factor (NGF), a member of the neurotrophin family, is essential for neuronal survival, differentiation and maturation. The aim of the present study was to investigate whether co-administration of GM1 and NGF reverses glutamate (Glu) neurotoxicity in primary cultured rat embryonic spinal cord neurons. Spinal cord neurons were exposed to Glu (2 mmol/l), Glu (2 mmol/l) plus GM1 (10 mg/ml), Glu (2 mmol/l) plus NGF (10 ng/ml), Glu (2 mmol/l) plus GM1 (5 mg/ml) and NGF (5 ng/ml) and then processed for detecting intracellular concentrations of Ca2+([Ca2+]i) by confocal laser scanning microscopy and growth associated protein 43 (GAP43) mRNA by RT-PCR. The fluorescent intensity in Glu plus GM1 and NGF incubated neurons was the lowest as compared with that in other groups. The expression of GAP43 mRNA in Glu plus GM1 and NGF incubated neurons was the highest as compared with that in other groups. These results implicated that GM1 and NGF have synergistic neuroprotective effects on spinal cord neurons with excitotoxicity induced by Glu in vitro. SN - 1873-2747 UR - https://www.unboundmedicine.com/medline/citation/19133317/The_neuroprotective_effects_of_NGF_combined_with_GM1_on_injured_spinal_cord_neurons_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0361-9230(08)00419-X DB - PRIME DP - Unbound Medicine ER -