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Human TLR3 recognizes dengue virus and modulates viral replication in vitro.
Cell Microbiol. 2009 Apr; 11(4):604-15.CM

Abstract

The elicitation of large amount inflammatory cytokine in serum has been developed as the cause of the plasma leakage in dengue fever (DF)/dengue haemorrhagic fever (DHF) infection. Virus recognition in innate immunity is the key. The Toll-like receptors (TLRs) play an important role in pathogen recognition towards cytokine induction among several viruses; however, the role of TLRs on innate immune recognition against DENV remains unclear. This study aims at the interaction between dengue virus (DENV) and human TLRs at the incipient stage of infection in vitro. Our experiment reveals that stably expression of TLR3, 7, 8 on HEK293 enables IL-8 secretion after DENV recognition. By the model of human monocytic cells U937, we demonstrated the trigger of IL-8 after viral recognition of human monocytic cell is primary through TLR3 following endosomal acidification. Silencing of TLR3 in U937 cells significantly blocks the DENV-induced IL-8 production. Besides, the interaction is further corroborated by colocalization of TLR3 and DENV RNA upon DENV internalization. Furthermore, in this study we found the expression of TLR3 can mediate strong IFN-alpha/beta release and inhibit DENV viral replication significantly, thus limit the cytopathic effect.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Tsai YT
Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Chang SY
No affiliation info available
Lee CN
No affiliation info available
Kao CL
No affiliation info available

MeSH

Cell LineDengueDengue VirusHost-Pathogen InteractionsHumansMonocytesToll-Like Receptor 3U937 CellsVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19134117

Citation

Tsai, Yi-Ting, et al. "Human TLR3 Recognizes Dengue Virus and Modulates Viral Replication in Vitro." Cellular Microbiology, vol. 11, no. 4, 2009, pp. 604-15.
Tsai YT, Chang SY, Lee CN, et al. Human TLR3 recognizes dengue virus and modulates viral replication in vitro. Cell Microbiol. 2009;11(4):604-15.
Tsai, Y. T., Chang, S. Y., Lee, C. N., & Kao, C. L. (2009). Human TLR3 recognizes dengue virus and modulates viral replication in vitro. Cellular Microbiology, 11(4), 604-15. https://doi.org/10.1111/j.1462-5822.2008.01277.x
Tsai YT, et al. Human TLR3 Recognizes Dengue Virus and Modulates Viral Replication in Vitro. Cell Microbiol. 2009;11(4):604-15. PubMed PMID: 19134117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human TLR3 recognizes dengue virus and modulates viral replication in vitro. AU - Tsai,Yi-Ting, AU - Chang,Sui-Yuan, AU - Lee,Chun-Nan, AU - Kao,Chuan-Liang, Y1 - 2009/02/04/ PY - 2009/1/13/entrez PY - 2009/1/13/pubmed PY - 2009/5/19/medline SP - 604 EP - 15 JF - Cellular microbiology JO - Cell Microbiol VL - 11 IS - 4 N2 - The elicitation of large amount inflammatory cytokine in serum has been developed as the cause of the plasma leakage in dengue fever (DF)/dengue haemorrhagic fever (DHF) infection. Virus recognition in innate immunity is the key. The Toll-like receptors (TLRs) play an important role in pathogen recognition towards cytokine induction among several viruses; however, the role of TLRs on innate immune recognition against DENV remains unclear. This study aims at the interaction between dengue virus (DENV) and human TLRs at the incipient stage of infection in vitro. Our experiment reveals that stably expression of TLR3, 7, 8 on HEK293 enables IL-8 secretion after DENV recognition. By the model of human monocytic cells U937, we demonstrated the trigger of IL-8 after viral recognition of human monocytic cell is primary through TLR3 following endosomal acidification. Silencing of TLR3 in U937 cells significantly blocks the DENV-induced IL-8 production. Besides, the interaction is further corroborated by colocalization of TLR3 and DENV RNA upon DENV internalization. Furthermore, in this study we found the expression of TLR3 can mediate strong IFN-alpha/beta release and inhibit DENV viral replication significantly, thus limit the cytopathic effect. SN - 1462-5822 UR - https://www.unboundmedicine.com/medline/citation/19134117/Human_TLR3_recognizes_dengue_virus_and_modulates_viral_replication_in_vitro_ L2 - https://doi.org/10.1111/j.1462-5822.2008.01277.x DB - PRIME DP - Unbound Medicine ER -