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The marsupial CD8 gene locus: molecular cloning and expression analysis of the alpha and beta sequences in the gray short-tailed opossum (Monodelphis domestica) and the tammar wallaby (Macropus eugenii).
Vet Immunol Immunopathol 2009; 129(1-2):14-27VI

Abstract

In eutherian mammals, CD8 is a key receptor of cytotoxic T cells and plays a pivotal role in the recognition and elimination of infected host cells by cell-mediated cytotoxicity. Here, we report the molecular cloning and expression analysis of CD8alpha and CD8beta cDNAs in two marsupial species, the gray short-tailed opossum and the tammar wallaby. The opossum and tammar CD8 sequences share a high degree of amino acid identity of 63% (CD8alpha) and 57% (CD8beta) to each other as well as 36-45% (CD8alpha) and 38-41% (CD8beta) with their eutherian counterparts. In addition, many of the signature features of eutherian CD8alpha and CD8beta are preserved in both marsupials including the two invariant cysteines that form the intra-chain disulphide bond in the extracellular IgSfV domain and the two hinge region cysteines involved in dimerisation between the two subunits. The p56(lck) binding motif in the cytoplasmic tail of the CD8alpha subunit is also conserved. Interestingly, the opossum CD8alpha and the tammar CD8beta sequences have a truncated cytoplasmic tail. RT-PCR analysis of CD8alpha and CD8beta transcripts in the tissues of the adult opossum and tammar showed broad tissue expression with a high level of expression observed in the lymphoid tissues of both marsupials. Furthermore, RT-PCR analysis of CD8alpha and CD8beta transcripts in the immune tissues of tammar young over the first 120 days of pouch life revealed a pattern of expression analogous to the maturation of the lymphoid tissues. This is the first report confirming the presence of CD8 in the tissues of a marsupial and will provide the tools to further analyse T cell subsets in this unique group of mammals.

Authors+Show Affiliations

Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, NSW, Australia. lduncan@bio.mq.edu.auNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19135263

Citation

Duncan, Louise G., et al. "The Marsupial CD8 Gene Locus: Molecular Cloning and Expression Analysis of the Alpha and Beta Sequences in the Gray Short-tailed Opossum (Monodelphis Domestica) and the Tammar Wallaby (Macropus Eugenii)." Veterinary Immunology and Immunopathology, vol. 129, no. 1-2, 2009, pp. 14-27.
Duncan LG, Nair SV, Deane EM. The marsupial CD8 gene locus: molecular cloning and expression analysis of the alpha and beta sequences in the gray short-tailed opossum (Monodelphis domestica) and the tammar wallaby (Macropus eugenii). Vet Immunol Immunopathol. 2009;129(1-2):14-27.
Duncan, L. G., Nair, S. V., & Deane, E. M. (2009). The marsupial CD8 gene locus: molecular cloning and expression analysis of the alpha and beta sequences in the gray short-tailed opossum (Monodelphis domestica) and the tammar wallaby (Macropus eugenii). Veterinary Immunology and Immunopathology, 129(1-2), pp. 14-27. doi:10.1016/j.vetimm.2008.12.003.
Duncan LG, Nair SV, Deane EM. The Marsupial CD8 Gene Locus: Molecular Cloning and Expression Analysis of the Alpha and Beta Sequences in the Gray Short-tailed Opossum (Monodelphis Domestica) and the Tammar Wallaby (Macropus Eugenii). Vet Immunol Immunopathol. 2009 May 15;129(1-2):14-27. PubMed PMID: 19135263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The marsupial CD8 gene locus: molecular cloning and expression analysis of the alpha and beta sequences in the gray short-tailed opossum (Monodelphis domestica) and the tammar wallaby (Macropus eugenii). AU - Duncan,Louise G, AU - Nair,Sham V, AU - Deane,Elizabeth M, Y1 - 2008/12/06/ PY - 2008/07/28/received PY - 2008/11/28/revised PY - 2008/12/01/accepted PY - 2009/1/13/entrez PY - 2009/1/13/pubmed PY - 2009/7/31/medline SP - 14 EP - 27 JF - Veterinary immunology and immunopathology JO - Vet. Immunol. Immunopathol. VL - 129 IS - 1-2 N2 - In eutherian mammals, CD8 is a key receptor of cytotoxic T cells and plays a pivotal role in the recognition and elimination of infected host cells by cell-mediated cytotoxicity. Here, we report the molecular cloning and expression analysis of CD8alpha and CD8beta cDNAs in two marsupial species, the gray short-tailed opossum and the tammar wallaby. The opossum and tammar CD8 sequences share a high degree of amino acid identity of 63% (CD8alpha) and 57% (CD8beta) to each other as well as 36-45% (CD8alpha) and 38-41% (CD8beta) with their eutherian counterparts. In addition, many of the signature features of eutherian CD8alpha and CD8beta are preserved in both marsupials including the two invariant cysteines that form the intra-chain disulphide bond in the extracellular IgSfV domain and the two hinge region cysteines involved in dimerisation between the two subunits. The p56(lck) binding motif in the cytoplasmic tail of the CD8alpha subunit is also conserved. Interestingly, the opossum CD8alpha and the tammar CD8beta sequences have a truncated cytoplasmic tail. RT-PCR analysis of CD8alpha and CD8beta transcripts in the tissues of the adult opossum and tammar showed broad tissue expression with a high level of expression observed in the lymphoid tissues of both marsupials. Furthermore, RT-PCR analysis of CD8alpha and CD8beta transcripts in the immune tissues of tammar young over the first 120 days of pouch life revealed a pattern of expression analogous to the maturation of the lymphoid tissues. This is the first report confirming the presence of CD8 in the tissues of a marsupial and will provide the tools to further analyse T cell subsets in this unique group of mammals. SN - 0165-2427 UR - https://www.unboundmedicine.com/medline/citation/19135263/The_marsupial_CD8_gene_locus:_molecular_cloning_and_expression_analysis_of_the_alpha_and_beta_sequences_in_the_gray_short_tailed_opossum__Monodelphis_domestica__and_the_tammar_wallaby__Macropus_eugenii__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(08)00777-0 DB - PRIME DP - Unbound Medicine ER -