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Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film.
Biosens Bioelectron. 2009 Apr 15; 24(8):2352-7.BB

Abstract

This study described the bioelectrochemistry of hemoglobin (Hb) at multiwall carbon nanotubes (MWCNTs) noncovalently functionalized with biopolymers of sodium alginate (SA). The characteristics of Hb/SA-MWCNTs composite film were studied by using FT-IR spectroscopy, UV-vis spectroscopy and electrochemical methods. Hb immobilized on SA-MWCNTs composite film retained its native secondary structure, achieved direct electron transfer with the apparent heterogeneous electron transfer rate constant (k(s)) of (9.54+/-0.883) s(-1) and showed excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide. The amperometric response of the biosensor varied linearly with the H(2)O(2) concentration ranging from 40 to 200 microM, with a detection limit of 16.41x10(-6) M (S/N=3) and the good long-term stability. Finally, we applied this proposed method to investigate the concentration of hydrogen peroxide in real samples.

Authors+Show Affiliations

Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19135354

Citation

Zhao, H Y., et al. "Bioelectrochemistry of Hemoglobin Immobilized On a Sodium Alginate-multiwall Carbon Nanotubes Composite Film." Biosensors & Bioelectronics, vol. 24, no. 8, 2009, pp. 2352-7.
Zhao HY, Zheng W, Meng ZX, et al. Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film. Biosens Bioelectron. 2009;24(8):2352-7.
Zhao, H. Y., Zheng, W., Meng, Z. X., Zhou, H. M., Xu, X. X., Li, Z., & Zheng, Y. F. (2009). Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film. Biosensors & Bioelectronics, 24(8), 2352-7. https://doi.org/10.1016/j.bios.2008.12.004
Zhao HY, et al. Bioelectrochemistry of Hemoglobin Immobilized On a Sodium Alginate-multiwall Carbon Nanotubes Composite Film. Biosens Bioelectron. 2009 Apr 15;24(8):2352-7. PubMed PMID: 19135354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film. AU - Zhao,H Y, AU - Zheng,W, AU - Meng,Z X, AU - Zhou,H M, AU - Xu,X X, AU - Li,Z, AU - Zheng,Y F, Y1 - 2008/12/09/ PY - 2008/10/10/received PY - 2008/12/02/revised PY - 2008/12/03/accepted PY - 2009/1/13/entrez PY - 2009/1/13/pubmed PY - 2009/5/27/medline SP - 2352 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 8 N2 - This study described the bioelectrochemistry of hemoglobin (Hb) at multiwall carbon nanotubes (MWCNTs) noncovalently functionalized with biopolymers of sodium alginate (SA). The characteristics of Hb/SA-MWCNTs composite film were studied by using FT-IR spectroscopy, UV-vis spectroscopy and electrochemical methods. Hb immobilized on SA-MWCNTs composite film retained its native secondary structure, achieved direct electron transfer with the apparent heterogeneous electron transfer rate constant (k(s)) of (9.54+/-0.883) s(-1) and showed excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide. The amperometric response of the biosensor varied linearly with the H(2)O(2) concentration ranging from 40 to 200 microM, with a detection limit of 16.41x10(-6) M (S/N=3) and the good long-term stability. Finally, we applied this proposed method to investigate the concentration of hydrogen peroxide in real samples. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19135354/Bioelectrochemistry_of_hemoglobin_immobilized_on_a_sodium_alginate_multiwall_carbon_nanotubes_composite_film_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00650-7 DB - PRIME DP - Unbound Medicine ER -