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[Effect of velvet antler polypeptide on peripheral nerve regeneration].

Abstract

OBJECTIVE

To investigate the velvet antler polypeptide (VAP) on sciatic nerve regeneration in rats through local administration and VAP-PLGA compound membrane.

METHODS

The 3, 15 mg/g of VAP-PLGA compound membrane were prepared by compounding VAP and PLGA, respectively. Seventy-two Wistar rats, male or female, aged 3-6 months and weighing (250 +/- 50) g, were selected to make the model of sciatic nerve section. Then, all rats were randomized into 4 groups (n = 18): group A in which nothing was given after anastomosis, group B in which 1 mL of VAP at the concentration of 10 mg/L was injected into the gastrocnemius muscle medial for every other day, group C in which 3 mg/g of VAP-PLGA compound membrane was given to the nerve anastomotic stoma and group D in which 15 mg/g of VAP-PLGA compound membrane was given to the nerve anastomotic stoma. The sciatic adhesion degree observation, electrophysiological examination, immunohistochemical staining and hemi-quantity calculation and horseradish peroxidase (HRP) retrograde tracing were conducted 2, 4 and 6 weeks after operation, respectively.

RESULTS

All rats survived to the end of the experiment, without foot ulcer or neuroma. Severer nervous adherence was observed in group A, mild adherence in group B, and no adherence in groups C and D 2, 4 and 6 weeks after operation, respectively. The recovery rate of the evoked potential of triceps surae in groups B, C and D was better than that in group A (P < 0.01), group D was superior to groups B and C (P < 0.05) at each time point. No significant difference between group B and group C (P > 0.05) 2, 4 weeks after operation was detected, but group C was superior to group B (P < 0.05) 6 weeks after operation. For the regenerative fiber axon and the expression of myelin sheath TGF-P1 and IGF antigen, the staining intensity in groups B, C and D was higher than that in group A at each time point (P < 0.05), and there were significant differences between group D and groups B and C 6 weeks after operation (P < 0.05), but no difference between groups B and C (P > 0.05). The HRP retrograde tracing showed that the myelinated nerve fiber stained by HRP gradually increased as time passed by and myelinated nerve fiber stained by HRP in groups B, C and D was much more than that in group A, and group D was superior to the other groups. No significant difference between group B and group C was detected.

CONCLUSION

To apply VAP through either local administration or VAP-PLGA compound membrane around the attached site of nerve anastomosis is capable of promoting nerve regeneration, which has an obvious dose-effect relationship with the dose of VAP. Meanwhile, VAP-PLGA compound membrane can prevent the nerve adhesion.

Authors+Show Affiliations

Department of Hand and Foot Surgery, First Hospital of Jilin University, Changchun Jilin 130021, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19137890

Citation

Lu, Laijin, et al. "[Effect of Velvet Antler Polypeptide On Peripheral Nerve Regeneration]." Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, vol. 22, no. 12, 2008, pp. 1458-61.
Lu L, Wang K, Li L, et al. [Effect of velvet antler polypeptide on peripheral nerve regeneration]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2008;22(12):1458-61.
Lu, L., Wang, K., Li, L., Xuan, Z., & Gong, X. (2008). [Effect of velvet antler polypeptide on peripheral nerve regeneration]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, 22(12), pp. 1458-61.
Lu L, et al. [Effect of Velvet Antler Polypeptide On Peripheral Nerve Regeneration]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2008;22(12):1458-61. PubMed PMID: 19137890.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of velvet antler polypeptide on peripheral nerve regeneration]. AU - Lu,Laijin, AU - Wang,Keli, AU - Li,Lijun, AU - Xuan,Zhaopeng, AU - Gong,Xu, PY - 2009/1/14/entrez PY - 2009/1/14/pubmed PY - 2009/6/13/medline SP - 1458 EP - 61 JF - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery JO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi VL - 22 IS - 12 N2 - OBJECTIVE: To investigate the velvet antler polypeptide (VAP) on sciatic nerve regeneration in rats through local administration and VAP-PLGA compound membrane. METHODS: The 3, 15 mg/g of VAP-PLGA compound membrane were prepared by compounding VAP and PLGA, respectively. Seventy-two Wistar rats, male or female, aged 3-6 months and weighing (250 +/- 50) g, were selected to make the model of sciatic nerve section. Then, all rats were randomized into 4 groups (n = 18): group A in which nothing was given after anastomosis, group B in which 1 mL of VAP at the concentration of 10 mg/L was injected into the gastrocnemius muscle medial for every other day, group C in which 3 mg/g of VAP-PLGA compound membrane was given to the nerve anastomotic stoma and group D in which 15 mg/g of VAP-PLGA compound membrane was given to the nerve anastomotic stoma. The sciatic adhesion degree observation, electrophysiological examination, immunohistochemical staining and hemi-quantity calculation and horseradish peroxidase (HRP) retrograde tracing were conducted 2, 4 and 6 weeks after operation, respectively. RESULTS: All rats survived to the end of the experiment, without foot ulcer or neuroma. Severer nervous adherence was observed in group A, mild adherence in group B, and no adherence in groups C and D 2, 4 and 6 weeks after operation, respectively. The recovery rate of the evoked potential of triceps surae in groups B, C and D was better than that in group A (P < 0.01), group D was superior to groups B and C (P < 0.05) at each time point. No significant difference between group B and group C (P > 0.05) 2, 4 weeks after operation was detected, but group C was superior to group B (P < 0.05) 6 weeks after operation. For the regenerative fiber axon and the expression of myelin sheath TGF-P1 and IGF antigen, the staining intensity in groups B, C and D was higher than that in group A at each time point (P < 0.05), and there were significant differences between group D and groups B and C 6 weeks after operation (P < 0.05), but no difference between groups B and C (P > 0.05). The HRP retrograde tracing showed that the myelinated nerve fiber stained by HRP gradually increased as time passed by and myelinated nerve fiber stained by HRP in groups B, C and D was much more than that in group A, and group D was superior to the other groups. No significant difference between group B and group C was detected. CONCLUSION: To apply VAP through either local administration or VAP-PLGA compound membrane around the attached site of nerve anastomosis is capable of promoting nerve regeneration, which has an obvious dose-effect relationship with the dose of VAP. Meanwhile, VAP-PLGA compound membrane can prevent the nerve adhesion. SN - 1002-1892 UR - https://www.unboundmedicine.com/medline/citation/19137890/[Effect_of_velvet_antler_polypeptide_on_peripheral_nerve_regeneration]_ DB - PRIME DP - Unbound Medicine ER -