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Variations in archaeal and bacterial diversity associated with the sulfate-methane transition zone in continental margin sediments (Santa Barbara Basin, California).
Appl Environ Microbiol. 2009 Mar; 75(6):1487-99.AE

Abstract

The sulfate-methane transition zone (SMTZ) is a widespread feature of continental margins, representing a diffusion-controlled interface where there is enhanced microbial activity. SMTZ microbial activity is commonly associated with the anaerobic oxidation of methane (AOM), which is carried out by syntrophic associations between sulfate-reducing bacteria and methane-oxidizing archaea. While our understanding of the microorganisms catalyzing AOM has advanced, the diversity and ecological role of the greater microbial assemblage associated with the SMTZ have not been well characterized. In this study, the microbial diversity above, within, and beneath the Santa Barbara Basin SMTZ was described. ANME-1-related archaeal phylotypes appear to be the primary methane oxidizers in the Santa Barbara Basin SMTZ, which was independently supported by exclusive recovery of related methyl coenzyme M reductase genes (mcrA). Sulfate-reducing Deltaproteobacteria phylotypes affiliated with the Desulfobacterales and Desulfosarcina-Desulfococcus clades were also enriched in the SMTZ, as confirmed by analysis of dissimilatory sulfite reductase (dsr) gene diversity. Statistical methods demonstrated that there was a close relationship between the microbial assemblages recovered from the two horizons associated with the geochemically defined SMTZ, which could be distinguished from microbial diversity recovered from the sulfate-replete overlying horizons and methane-rich sediment beneath the transition zone. Comparison of the Santa Barbara Basin SMTZ microbial assemblage to microbial assemblages of methane seeps and other organic matter-rich sedimentary environments suggests that bacterial groups not typically associated with AOM, such as Planctomycetes and candidate division JS1, are additionally enriched within the SMTZ and may represent a common bacterial signature of many SMTZ environments worldwide.

Authors+Show Affiliations

Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

19139232

Citation

Harrison, Benjamin K., et al. "Variations in Archaeal and Bacterial Diversity Associated With the Sulfate-methane Transition Zone in Continental Margin Sediments (Santa Barbara Basin, California)." Applied and Environmental Microbiology, vol. 75, no. 6, 2009, pp. 1487-99.
Harrison BK, Zhang H, Berelson W, et al. Variations in archaeal and bacterial diversity associated with the sulfate-methane transition zone in continental margin sediments (Santa Barbara Basin, California). Appl Environ Microbiol. 2009;75(6):1487-99.
Harrison, B. K., Zhang, H., Berelson, W., & Orphan, V. J. (2009). Variations in archaeal and bacterial diversity associated with the sulfate-methane transition zone in continental margin sediments (Santa Barbara Basin, California). Applied and Environmental Microbiology, 75(6), 1487-99. https://doi.org/10.1128/AEM.01812-08
Harrison BK, et al. Variations in Archaeal and Bacterial Diversity Associated With the Sulfate-methane Transition Zone in Continental Margin Sediments (Santa Barbara Basin, California). Appl Environ Microbiol. 2009;75(6):1487-99. PubMed PMID: 19139232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variations in archaeal and bacterial diversity associated with the sulfate-methane transition zone in continental margin sediments (Santa Barbara Basin, California). AU - Harrison,Benjamin K, AU - Zhang,Husen, AU - Berelson,Will, AU - Orphan,Victoria J, Y1 - 2009/01/09/ PY - 2009/1/14/entrez PY - 2009/1/14/pubmed PY - 2009/5/16/medline SP - 1487 EP - 99 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 75 IS - 6 N2 - The sulfate-methane transition zone (SMTZ) is a widespread feature of continental margins, representing a diffusion-controlled interface where there is enhanced microbial activity. SMTZ microbial activity is commonly associated with the anaerobic oxidation of methane (AOM), which is carried out by syntrophic associations between sulfate-reducing bacteria and methane-oxidizing archaea. While our understanding of the microorganisms catalyzing AOM has advanced, the diversity and ecological role of the greater microbial assemblage associated with the SMTZ have not been well characterized. In this study, the microbial diversity above, within, and beneath the Santa Barbara Basin SMTZ was described. ANME-1-related archaeal phylotypes appear to be the primary methane oxidizers in the Santa Barbara Basin SMTZ, which was independently supported by exclusive recovery of related methyl coenzyme M reductase genes (mcrA). Sulfate-reducing Deltaproteobacteria phylotypes affiliated with the Desulfobacterales and Desulfosarcina-Desulfococcus clades were also enriched in the SMTZ, as confirmed by analysis of dissimilatory sulfite reductase (dsr) gene diversity. Statistical methods demonstrated that there was a close relationship between the microbial assemblages recovered from the two horizons associated with the geochemically defined SMTZ, which could be distinguished from microbial diversity recovered from the sulfate-replete overlying horizons and methane-rich sediment beneath the transition zone. Comparison of the Santa Barbara Basin SMTZ microbial assemblage to microbial assemblages of methane seeps and other organic matter-rich sedimentary environments suggests that bacterial groups not typically associated with AOM, such as Planctomycetes and candidate division JS1, are additionally enriched within the SMTZ and may represent a common bacterial signature of many SMTZ environments worldwide. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/19139232/Variations_in_archaeal_and_bacterial_diversity_associated_with_the_sulfate_methane_transition_zone_in_continental_margin_sediments__Santa_Barbara_Basin_California__ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=19139232 DB - PRIME DP - Unbound Medicine ER -