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Functional genetic variation in the basal promoter of the organic cation/carnitine transporters OCTN1 (SLC22A4) and OCTN2 (SLC22A5).
J Pharmacol Exp Ther. 2009 Apr; 329(1):262-71.JP

Abstract

The organic cation/ergothioneine transporter OCTN1 (SLC22A4) and the high-affinity carnitine transporter OCTN2 (SLC22A5), play an important role in the disposition of xenobiotics and endogenous compounds. Here, we analyzed the sequence of the proximal promoter regions of OCTN1 and OCTN2 in four ethnic groups and determined the effects of the identified genetic variants on transcriptional activities and mRNA expression. Six variants were found in the proximal promoter of OCTN1, one of which showed high allele frequency ranging from 13 to 34% in samples from individuals with ancestries in Africa, Europe, China, and Mexico. OCTN1 haplotypes had similar activities as the reference in luciferase reporter assays. For OCTN2, three of the seven variants identified in the proximal promoter showed allele frequencies greater than 29.5% in all populations, with the exception of -207C>G (rs2631367) that was monomorphic in Asian Americans. OCTN2 haplotypes containing -207G, present in all populations, were associated with a gain of function in luciferase reporter assays. Consistent with reporter assays, OCTN2 mRNA expression levels in lymphoblastoid cell lines (LCLs) from gene expression analysis were greater in samples carrying a marker for -207G. This SNP seems to contribute to racial differences in OCTN2 mRNA expression levels in LCLs. Our study with healthy subjects (n = 16) homozygous for either -207C or -207G, showed no appreciable effect of this SNP on carnitine disposition. However, there were significant effects of gender on carnitine plasma levels (p < 0.01). Further in vivo studies of OCTN2 promoter variants on carnitine disposition and variation in drug response are warranted.

Authors+Show Affiliations

Department of Biopharmaceutical Sciences, University of California, San Francisco, San Francisco, CA 94158-2911, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19141711

Citation

Tahara, Harunobu, et al. "Functional Genetic Variation in the Basal Promoter of the Organic Cation/carnitine Transporters OCTN1 (SLC22A4) and OCTN2 (SLC22A5)." The Journal of Pharmacology and Experimental Therapeutics, vol. 329, no. 1, 2009, pp. 262-71.
Tahara H, Yee SW, Urban TJ, et al. Functional genetic variation in the basal promoter of the organic cation/carnitine transporters OCTN1 (SLC22A4) and OCTN2 (SLC22A5). J Pharmacol Exp Ther. 2009;329(1):262-71.
Tahara, H., Yee, S. W., Urban, T. J., Hesselson, S., Castro, R. A., Kawamoto, M., Stryke, D., Johns, S. J., Ferrin, T. E., Kwok, P. Y., & Giacomini, K. M. (2009). Functional genetic variation in the basal promoter of the organic cation/carnitine transporters OCTN1 (SLC22A4) and OCTN2 (SLC22A5). The Journal of Pharmacology and Experimental Therapeutics, 329(1), 262-71. https://doi.org/10.1124/jpet.108.146449
Tahara H, et al. Functional Genetic Variation in the Basal Promoter of the Organic Cation/carnitine Transporters OCTN1 (SLC22A4) and OCTN2 (SLC22A5). J Pharmacol Exp Ther. 2009;329(1):262-71. PubMed PMID: 19141711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional genetic variation in the basal promoter of the organic cation/carnitine transporters OCTN1 (SLC22A4) and OCTN2 (SLC22A5). AU - Tahara,Harunobu, AU - Yee,Sook Wah, AU - Urban,Thomas J, AU - Hesselson,Stephanie, AU - Castro,Richard A, AU - Kawamoto,Michiko, AU - Stryke,Doug, AU - Johns,Susan J, AU - Ferrin,Thomas E, AU - Kwok,Pui-Yan, AU - Giacomini,Kathleen M, Y1 - 2009/01/13/ PY - 2009/1/15/entrez PY - 2009/1/15/pubmed PY - 2009/4/29/medline SP - 262 EP - 71 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 329 IS - 1 N2 - The organic cation/ergothioneine transporter OCTN1 (SLC22A4) and the high-affinity carnitine transporter OCTN2 (SLC22A5), play an important role in the disposition of xenobiotics and endogenous compounds. Here, we analyzed the sequence of the proximal promoter regions of OCTN1 and OCTN2 in four ethnic groups and determined the effects of the identified genetic variants on transcriptional activities and mRNA expression. Six variants were found in the proximal promoter of OCTN1, one of which showed high allele frequency ranging from 13 to 34% in samples from individuals with ancestries in Africa, Europe, China, and Mexico. OCTN1 haplotypes had similar activities as the reference in luciferase reporter assays. For OCTN2, three of the seven variants identified in the proximal promoter showed allele frequencies greater than 29.5% in all populations, with the exception of -207C>G (rs2631367) that was monomorphic in Asian Americans. OCTN2 haplotypes containing -207G, present in all populations, were associated with a gain of function in luciferase reporter assays. Consistent with reporter assays, OCTN2 mRNA expression levels in lymphoblastoid cell lines (LCLs) from gene expression analysis were greater in samples carrying a marker for -207G. This SNP seems to contribute to racial differences in OCTN2 mRNA expression levels in LCLs. Our study with healthy subjects (n = 16) homozygous for either -207C or -207G, showed no appreciable effect of this SNP on carnitine disposition. However, there were significant effects of gender on carnitine plasma levels (p < 0.01). Further in vivo studies of OCTN2 promoter variants on carnitine disposition and variation in drug response are warranted. SN - 1521-0103 UR - https://www.unboundmedicine.com/medline/citation/19141711/Functional_genetic_variation_in_the_basal_promoter_of_the_organic_cation/carnitine_transporters_OCTN1__SLC22A4__and_OCTN2__SLC22A5__ L2 - https://jpet.aspetjournals.org/cgi/pmidlookup?view=long&amp;pmid=19141711 DB - PRIME DP - Unbound Medicine ER -