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Capillary electrochromatographic separation of illicit drugs employing a cyano stationary phase.
J Chromatogr A. 2009 Apr 24; 1216(17):3652-9.JC

Abstract

In this study, we present a capillary electrochromatographic method for separation of basic compounds of interest in forensic science (amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, cocaine, codeine, heroin, morphine, and 6-monoacethylmorphine). Several analytical conditions were taken into account to completely separate in the same run the 10 drugs of abuse analyzed. Chromatographic retention, selectivity and efficiency were evaluated in dependence of the type of stationary phase (CN and RP-C(18) derivatized silica particles), mobile phase composition, buffer type and pH, sample injection. The optimum separation parameters were set up using a mixture of aqueous sodium phosphate buffer (pH 2.5)/acetonitrile (80/20, v/v) as the mobile phase, 10 kV and 20 degrees C as applied voltage and capillary temperature, respectively. Under these conditions all the studied analytes were baseline resolved within 20 min. The method performance was investigated in terms of precision, linearity, sensitivity and accuracy to demonstrate the applicability of the developed capillary electrochromatographic system to forensic analysis. Calibration curves provided a good linearity over a working range of 100-1200 ng/mL for all analytes. Limits of detection and quantification were in the range 5-12 ng/mL and 10-30 ng/mL, respectively. Then the method was applied to the analysis of a human urine sample spiked with a basic compounds' mixture. Urine samples' pre-treatment was carried out through a solid phase extraction (SPE) procedure on strong cation exchange (SCX) cartridges.

Authors+Show Affiliations

Istituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche, Monterotondo Scalo Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

19144341

Citation

Aturki, Zeineb, et al. "Capillary Electrochromatographic Separation of Illicit Drugs Employing a Cyano Stationary Phase." Journal of Chromatography. A, vol. 1216, no. 17, 2009, pp. 3652-9.
Aturki Z, D'Orazio G, Fanali S, et al. Capillary electrochromatographic separation of illicit drugs employing a cyano stationary phase. J Chromatogr A. 2009;1216(17):3652-9.
Aturki, Z., D'Orazio, G., Fanali, S., Rocco, A., Bortolotti, F., Gottardo, R., & Tagliaro, F. (2009). Capillary electrochromatographic separation of illicit drugs employing a cyano stationary phase. Journal of Chromatography. A, 1216(17), 3652-9. https://doi.org/10.1016/j.chroma.2008.12.041
Aturki Z, et al. Capillary Electrochromatographic Separation of Illicit Drugs Employing a Cyano Stationary Phase. J Chromatogr A. 2009 Apr 24;1216(17):3652-9. PubMed PMID: 19144341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrochromatographic separation of illicit drugs employing a cyano stationary phase. AU - Aturki,Zeineb, AU - D'Orazio,Giovanni, AU - Fanali,Salvatore, AU - Rocco,Anna, AU - Bortolotti,Federica, AU - Gottardo,Rossella, AU - Tagliaro,Franco, Y1 - 2008/12/25/ PY - 2008/10/27/received PY - 2008/12/03/revised PY - 2008/12/15/accepted PY - 2009/1/16/entrez PY - 2009/1/16/pubmed PY - 2009/7/25/medline SP - 3652 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 17 N2 - In this study, we present a capillary electrochromatographic method for separation of basic compounds of interest in forensic science (amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, cocaine, codeine, heroin, morphine, and 6-monoacethylmorphine). Several analytical conditions were taken into account to completely separate in the same run the 10 drugs of abuse analyzed. Chromatographic retention, selectivity and efficiency were evaluated in dependence of the type of stationary phase (CN and RP-C(18) derivatized silica particles), mobile phase composition, buffer type and pH, sample injection. The optimum separation parameters were set up using a mixture of aqueous sodium phosphate buffer (pH 2.5)/acetonitrile (80/20, v/v) as the mobile phase, 10 kV and 20 degrees C as applied voltage and capillary temperature, respectively. Under these conditions all the studied analytes were baseline resolved within 20 min. The method performance was investigated in terms of precision, linearity, sensitivity and accuracy to demonstrate the applicability of the developed capillary electrochromatographic system to forensic analysis. Calibration curves provided a good linearity over a working range of 100-1200 ng/mL for all analytes. Limits of detection and quantification were in the range 5-12 ng/mL and 10-30 ng/mL, respectively. Then the method was applied to the analysis of a human urine sample spiked with a basic compounds' mixture. Urine samples' pre-treatment was carried out through a solid phase extraction (SPE) procedure on strong cation exchange (SCX) cartridges. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19144341/Capillary_electrochromatographic_separation_of_illicit_drugs_employing_a_cyano_stationary_phase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)02202-4 DB - PRIME DP - Unbound Medicine ER -