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Improved detection of Histoplasma antigenemia following dissociation of immune complexes.
Clin Vaccine Immunol. 2009 Mar; 16(3):320-2.CV

Abstract

The sensitivity for detection of Histoplasma antigen is lower in serum than in urine. In other antigen assays, treatment of serum at 104 degrees C in the presence of EDTA was required for detection of antigenemia. Sensitivity and specificity for detection of Histoplasma antigenemia were examined with or without EDTA heat treatment of the serum using the MVista Histoplasma antigen enzyme immunoassay. A total of 94.6% of serum specimens from patients with AIDS and histoplasmosis that were negative untreated were positive after EDTA-heat treatment. Two-thirds of the negative serum specimens from patients with probable histoplasmosis, based upon clinical suspicion and Histoplasma antigenuria, were positive after heat treatment. Specificity was 99.0% in controls, including healthy subjects and patients in whom histoplasmosis or blastomycosis, were excluded. Precision and reproducibility were good and excellent, respectively. These findings demonstrate improvement in sensitivity without reduction in specificity, precision, or reproducibility after heat-EDTA treatment.

Authors+Show Affiliations

MiraVista Diagnostics, Indianapolis, Indiana 46241, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19144790

Citation

Swartzentruber, S, et al. "Improved Detection of Histoplasma Antigenemia Following Dissociation of Immune Complexes." Clinical and Vaccine Immunology : CVI, vol. 16, no. 3, 2009, pp. 320-2.
Swartzentruber S, LeMonte A, Witt J, et al. Improved detection of Histoplasma antigenemia following dissociation of immune complexes. Clin Vaccine Immunol. 2009;16(3):320-2.
Swartzentruber, S., LeMonte, A., Witt, J., Fuller, D., Davis, T., Hage, C., Connolly, P., Durkin, M., & Wheat, L. J. (2009). Improved detection of Histoplasma antigenemia following dissociation of immune complexes. Clinical and Vaccine Immunology : CVI, 16(3), 320-2. https://doi.org/10.1128/CVI.00409-08
Swartzentruber S, et al. Improved Detection of Histoplasma Antigenemia Following Dissociation of Immune Complexes. Clin Vaccine Immunol. 2009;16(3):320-2. PubMed PMID: 19144790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved detection of Histoplasma antigenemia following dissociation of immune complexes. AU - Swartzentruber,S, AU - LeMonte,A, AU - Witt,J, AU - Fuller,D, AU - Davis,T, AU - Hage,C, AU - Connolly,P, AU - Durkin,M, AU - Wheat,L J, Y1 - 2009/01/14/ PY - 2009/1/16/entrez PY - 2009/1/16/pubmed PY - 2009/4/14/medline SP - 320 EP - 2 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 16 IS - 3 N2 - The sensitivity for detection of Histoplasma antigen is lower in serum than in urine. In other antigen assays, treatment of serum at 104 degrees C in the presence of EDTA was required for detection of antigenemia. Sensitivity and specificity for detection of Histoplasma antigenemia were examined with or without EDTA heat treatment of the serum using the MVista Histoplasma antigen enzyme immunoassay. A total of 94.6% of serum specimens from patients with AIDS and histoplasmosis that were negative untreated were positive after EDTA-heat treatment. Two-thirds of the negative serum specimens from patients with probable histoplasmosis, based upon clinical suspicion and Histoplasma antigenuria, were positive after heat treatment. Specificity was 99.0% in controls, including healthy subjects and patients in whom histoplasmosis or blastomycosis, were excluded. Precision and reproducibility were good and excellent, respectively. These findings demonstrate improvement in sensitivity without reduction in specificity, precision, or reproducibility after heat-EDTA treatment. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/19144790/Improved_detection_of_Histoplasma_antigenemia_following_dissociation_of_immune_complexes_ L2 - https://journals.asm.org/doi/10.1128/CVI.00409-08?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -