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Association of unique, isolated treponemes with bovine digital dermatitis lesions.
J Clin Microbiol. 2009 Mar; 47(3):689-96.JC

Abstract

This study used a PCR-based approach targeting 16S rRNA gene fragments to determine the occurrence and association of the three bovine digital dermatitis (BDD) treponeme phylogroups within lesions found in cattle from the United Kingdom. Examination of 51 BDD lesions collected from infected cattle across the United Kingdom revealed that BDD treponeme group 1 (Treponema medium/Treponema vincentii-like), group 2 (Treponema phagedenis-like), and group 3 (Treponema putidum/Treponema denticola-like) were present in 96.1%, 98%, and 76.5% of BDD lesions, respectively. The three phylogroups were present together in 74.5% of lesions. The PCR assays enabled the isolation of further treponeme strains from previously mixed primary BDD lesion cultures. Here a representative from each of the three distinct treponeme phylogroups was isolated from a single BDD lesion for the first time. These data highlight the extent to which this disease is polytreponemal. Immunohistochemistry and electron microscopy were used to investigate lesional hoof tissues, resulting in treponemes being identified copiously in hair follicles and sebaceous glands, suggesting a potential route of exit and/or entry for these pathogens. This study gives further evidence for the importance of the three treponeme groups in BDD pathogenesis and reiterates the value of molecular genetic approaches for isolating and identifying fastidious anaerobes.

Authors+Show Affiliations

Department of Veterinary Pathology, Faculty of Veterinary Science, University of Liverpool, Liverpool L69 3BX, United Kingdom. evansnj@liverpool.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19144804

Citation

Evans, Nicholas J., et al. "Association of Unique, Isolated Treponemes With Bovine Digital Dermatitis Lesions." Journal of Clinical Microbiology, vol. 47, no. 3, 2009, pp. 689-96.
Evans NJ, Brown JM, Demirkan I, et al. Association of unique, isolated treponemes with bovine digital dermatitis lesions. J Clin Microbiol. 2009;47(3):689-96.
Evans, N. J., Brown, J. M., Demirkan, I., Singh, P., Getty, B., Timofte, D., Vink, W. D., Murray, R. D., Blowey, R. W., Birtles, R. J., Hart, C. A., & Carter, S. D. (2009). Association of unique, isolated treponemes with bovine digital dermatitis lesions. Journal of Clinical Microbiology, 47(3), 689-96. https://doi.org/10.1128/JCM.01914-08
Evans NJ, et al. Association of Unique, Isolated Treponemes With Bovine Digital Dermatitis Lesions. J Clin Microbiol. 2009;47(3):689-96. PubMed PMID: 19144804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of unique, isolated treponemes with bovine digital dermatitis lesions. AU - Evans,Nicholas J, AU - Brown,Jennifer M, AU - Demirkan,Ibrahim, AU - Singh,Prem, AU - Getty,Brian, AU - Timofte,Dorina, AU - Vink,W Daan, AU - Murray,Richard D, AU - Blowey,Roger W, AU - Birtles,Richard J, AU - Hart,C Anthony, AU - Carter,Stuart D, Y1 - 2009/01/14/ PY - 2009/1/16/entrez PY - 2009/1/16/pubmed PY - 2009/3/31/medline SP - 689 EP - 96 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 47 IS - 3 N2 - This study used a PCR-based approach targeting 16S rRNA gene fragments to determine the occurrence and association of the three bovine digital dermatitis (BDD) treponeme phylogroups within lesions found in cattle from the United Kingdom. Examination of 51 BDD lesions collected from infected cattle across the United Kingdom revealed that BDD treponeme group 1 (Treponema medium/Treponema vincentii-like), group 2 (Treponema phagedenis-like), and group 3 (Treponema putidum/Treponema denticola-like) were present in 96.1%, 98%, and 76.5% of BDD lesions, respectively. The three phylogroups were present together in 74.5% of lesions. The PCR assays enabled the isolation of further treponeme strains from previously mixed primary BDD lesion cultures. Here a representative from each of the three distinct treponeme phylogroups was isolated from a single BDD lesion for the first time. These data highlight the extent to which this disease is polytreponemal. Immunohistochemistry and electron microscopy were used to investigate lesional hoof tissues, resulting in treponemes being identified copiously in hair follicles and sebaceous glands, suggesting a potential route of exit and/or entry for these pathogens. This study gives further evidence for the importance of the three treponeme groups in BDD pathogenesis and reiterates the value of molecular genetic approaches for isolating and identifying fastidious anaerobes. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/19144804/Association_of_unique_isolated_treponemes_with_bovine_digital_dermatitis_lesions_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=19144804 DB - PRIME DP - Unbound Medicine ER -