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Detection of Illicium anisatum as adulterant of Illicium verum.
Planta Med. 2009 Mar; 75(4):392-5.PM

Abstract

Chinese Star anise, Illicium verum Hook, is a well known spice in many cultures and has also been used to treat infant colic. Recent publications report that Chinese Star anise might be adulterated with the toxic Japanese Star anise, Illicium anisatum L. We have developed a molecular method that helps with the detection of I. anisatum as adulterant of I. verum. We PCR-amplified the internal transcribed spacer (ITS) region and analyzed it with the endonucleases PSTI and BFMI. Based on fragment length polymorphisms (RFLP), we were able to detect and distinguish between I. anisatum and other Illicium species in powdered samples.

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Authors+Show Affiliations

Techen N
National Center for Natural Products Research and Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS 38677, USA. ntechen@olemiss.edu
Pan Z
No affiliation info available
Scheffler BE
No affiliation info available
Khan IA
No affiliation info available

MeSH

Base SequenceDNA, IntergenicDNA, PlantFood ContaminationIlliciumPlants, ToxicSpecies Specificity

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

19145553

Citation

Techen, Natascha, et al. "Detection of Illicium Anisatum as Adulterant of Illicium Verum." Planta Medica, vol. 75, no. 4, 2009, pp. 392-5.
Techen N, Pan Z, Scheffler BE, et al. Detection of Illicium anisatum as adulterant of Illicium verum. Planta Med. 2009;75(4):392-5.
Techen, N., Pan, Z., Scheffler, B. E., & Khan, I. A. (2009). Detection of Illicium anisatum as adulterant of Illicium verum. Planta Medica, 75(4), 392-5. https://doi.org/10.1055/s-0028-1112219
Techen N, et al. Detection of Illicium Anisatum as Adulterant of Illicium Verum. Planta Med. 2009;75(4):392-5. PubMed PMID: 19145553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Illicium anisatum as adulterant of Illicium verum. AU - Techen,Natascha, AU - Pan,Zhiqiang, AU - Scheffler,Brian E, AU - Khan,Ikhlas A, Y1 - 2009/01/14/ PY - 2009/1/16/entrez PY - 2009/1/16/pubmed PY - 2009/5/21/medline SP - 392 EP - 5 JF - Planta medica JO - Planta Med VL - 75 IS - 4 N2 - Chinese Star anise, Illicium verum Hook, is a well known spice in many cultures and has also been used to treat infant colic. Recent publications report that Chinese Star anise might be adulterated with the toxic Japanese Star anise, Illicium anisatum L. We have developed a molecular method that helps with the detection of I. anisatum as adulterant of I. verum. We PCR-amplified the internal transcribed spacer (ITS) region and analyzed it with the endonucleases PSTI and BFMI. Based on fragment length polymorphisms (RFLP), we were able to detect and distinguish between I. anisatum and other Illicium species in powdered samples. SN - 0032-0943 UR - https://www.unboundmedicine.com/medline/citation/19145553/Detection_of_Illicium_anisatum_as_adulterant_of_Illicium_verum_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0028-1112219 DB - PRIME DP - Unbound Medicine ER -