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[Effects of ghrelin on the proliferation and differentiation of 3T3-L1 preadipocytes and its possible mechanisms].
Zhongguo Dang Dai Er Ke Za Zhi. 2009 Jan; 11(1):69-73.ZD

Abstract

OBJECTIVE

To investigate the effects of ghrelin on the proliferation and differentiation of 3T3-L1 preadipocyte, and study the possible mechanisms.

METHODS

3T3-L1 preadipocytes were cultured in vitro. The proliferation potentials of 3T3-L1 preadipocytes that were treated with different concentrations of ghrelin were evaluated by MTT methods. The levels of c-myc and thymidine kinase mRNA were detected using RT-PCR. 3T3-L1 preadipocytes were differentiated into the matured adipocytes with insulin (INS) or ghrelin. The morphological changes of 3T3-L1 adipocytes were observed and the differentiation rate was assayed by oil-red O staining. Total RNA was extracted from adipocytes at various times, and the levels of peroxisome proliferation activated receptor gamma (PPARgamma) and CAAT/enhancer binding protein(C/EBPalpha) mRNA expressions were detected using RT-PCR.

RESULTS

Ghrelin at concentrations of 10(-7) to 10(-15) mol/L significantly stimulated preadipocyte proliferation (p<0.05). The levels of c-myc and thymidine kinase mRNA significantly increased in 3T3-L1 preadipocytes with 10(-9) mol/L and 10(-11) mol/L ghrelin treatment (p<0.01). The 3T3-L1 preadipocytes treated with 10(-11) mol/L ghrelin had lots of lipid droplets in the cytoplasma, but the differentiation rate was lower than those treated with INS. Ghrelin of 10(-11) mol/L significantly increased the mRNA expression of PPARgamma and C/EBPalpha in the course of 3T3-L1 preadipocyte differentiation, compared with the normal control group (p<0.05). The PPARgamma and C/EBPalpha mRNA expression increased with the prolonged differentiation of preadipocytes induced by ghrelin or INS. There were significant differences in the levels of PPARgamma and C/EBPalpha mRNA expression between the 2nd and 8th days of differentiation(p<0.01).

CONCLUSIONS

Ghrelin promotes the proliferation and differentiation of 3T3-L1 preadipocytes. The proliferation of 3T3-L1 preadipocytes induced by ghrelin may be associated with increased c-myc levels. Ghrelin may promote differentiation of 3T3-L1 preadipocytes by increasing mRNA expression of PPARgamma and C/EBPalpha, thus enhances the sensitivity of adipocytes to INS.

Authors+Show Affiliations

Liu J
Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Lin HH
No affiliation info available
Cheng PX
No affiliation info available
Hu XF
No affiliation info available
Lu HL
No affiliation info available

MeSH

3T3-L1 CellsAdipocytesAnimalsCCAAT-Enhancer-Binding Protein-alphaCell DifferentiationCell ProliferationGenes, mycGhrelinMicePPAR gammaRNA, MessengerStem CellsThymidine Kinase

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19149928

Citation

Liu, Jing, et al. "[Effects of Ghrelin On the Proliferation and Differentiation of 3T3-L1 Preadipocytes and Its Possible Mechanisms]." Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, vol. 11, no. 1, 2009, pp. 69-73.
Liu J, Lin HH, Cheng PX, et al. [Effects of ghrelin on the proliferation and differentiation of 3T3-L1 preadipocytes and its possible mechanisms]. Zhongguo Dang Dai Er Ke Za Zhi. 2009;11(1):69-73.
Liu, J., Lin, H. H., Cheng, P. X., Hu, X. F., & Lu, H. L. (2009). [Effects of ghrelin on the proliferation and differentiation of 3T3-L1 preadipocytes and its possible mechanisms]. Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, 11(1), 69-73.
Liu J, et al. [Effects of Ghrelin On the Proliferation and Differentiation of 3T3-L1 Preadipocytes and Its Possible Mechanisms]. Zhongguo Dang Dai Er Ke Za Zhi. 2009;11(1):69-73. PubMed PMID: 19149928.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of ghrelin on the proliferation and differentiation of 3T3-L1 preadipocytes and its possible mechanisms]. AU - Liu,Jing, AU - Lin,Han-Hua, AU - Cheng,Pei-Xuan, AU - Hu,Xiu-Fen, AU - Lu,Hui-Ling, PY - 2009/1/20/entrez PY - 2009/1/20/pubmed PY - 2009/3/25/medline SP - 69 EP - 73 JF - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics JO - Zhongguo Dang Dai Er Ke Za Zhi VL - 11 IS - 1 N2 - OBJECTIVE: To investigate the effects of ghrelin on the proliferation and differentiation of 3T3-L1 preadipocyte, and study the possible mechanisms. METHODS: 3T3-L1 preadipocytes were cultured in vitro. The proliferation potentials of 3T3-L1 preadipocytes that were treated with different concentrations of ghrelin were evaluated by MTT methods. The levels of c-myc and thymidine kinase mRNA were detected using RT-PCR. 3T3-L1 preadipocytes were differentiated into the matured adipocytes with insulin (INS) or ghrelin. The morphological changes of 3T3-L1 adipocytes were observed and the differentiation rate was assayed by oil-red O staining. Total RNA was extracted from adipocytes at various times, and the levels of peroxisome proliferation activated receptor gamma (PPARgamma) and CAAT/enhancer binding protein(C/EBPalpha) mRNA expressions were detected using RT-PCR. RESULTS: Ghrelin at concentrations of 10(-7) to 10(-15) mol/L significantly stimulated preadipocyte proliferation (p<0.05). The levels of c-myc and thymidine kinase mRNA significantly increased in 3T3-L1 preadipocytes with 10(-9) mol/L and 10(-11) mol/L ghrelin treatment (p<0.01). The 3T3-L1 preadipocytes treated with 10(-11) mol/L ghrelin had lots of lipid droplets in the cytoplasma, but the differentiation rate was lower than those treated with INS. Ghrelin of 10(-11) mol/L significantly increased the mRNA expression of PPARgamma and C/EBPalpha in the course of 3T3-L1 preadipocyte differentiation, compared with the normal control group (p<0.05). The PPARgamma and C/EBPalpha mRNA expression increased with the prolonged differentiation of preadipocytes induced by ghrelin or INS. There were significant differences in the levels of PPARgamma and C/EBPalpha mRNA expression between the 2nd and 8th days of differentiation(p<0.01). CONCLUSIONS: Ghrelin promotes the proliferation and differentiation of 3T3-L1 preadipocytes. The proliferation of 3T3-L1 preadipocytes induced by ghrelin may be associated with increased c-myc levels. Ghrelin may promote differentiation of 3T3-L1 preadipocytes by increasing mRNA expression of PPARgamma and C/EBPalpha, thus enhances the sensitivity of adipocytes to INS. SN - 1008-8830 UR - https://www.unboundmedicine.com/medline/citation/19149928/[Effects_of_ghrelin_on_the_proliferation_and_differentiation_of_3T3_L1_preadipocytes_and_its_possible_mechanisms]_ L2 - https://medlineplus.gov/stemcells.html DB - PRIME DP - Unbound Medicine ER -